The transdermal absorption by tissues of salicylate from triethanolamine salicylate (TEA/S) ointment applied to canine and human skin was shown to be consistent, significant, and reproducible. Salicylate levels were measured using thin-layer chromatography, nuclear magnetic resonance, and radiochemistry. Absorption was directly proportional to the concentrations of the active ingredient in the ointment up to the 10 per cent preparation. Tissue salicylate levels were not influenced by the sex of the canine subjects. In humans, the activity of the patients affected both tissue levels and urinary excretion. Autoradiographic and doubly labeled studies suggest that during transdermal absorption of TEA/S, the salt may disassociate; this could permit the salicylate to have a longer transit time in the area of application.

Animals↗

PubMedDate not supplied

A probable defect in the beta-oxidation of lipids in rats fed alcohol for 6 months.

Studies on fatty acid oxidation were made in rats fed for 6 months on a liquid diet containing 15% total calories as ethanol. After 6 months, a marked diminution was observed in the in vivo production of 14CO2 from labeled palmitate and octanoate in the ethanol-fed animals compared to their pair-fed isocaloric controls not receiving alcohol. Similar results were obtained in 14CO2 formation from 14C-fatty acids using liver mitochondria from these ethanol-fed rats after 6 months. The effect on octanoate beta-oxidation was larger than that for palmitate. Addition of purified acyl CoA dehydrogenase complexes and additional electron transfer flavoprotein complex to the mitochondria suggested that the ethanol-fed animals could have been deficient in the medium-chain acyl CoA-dehydrogenase complex.

Alcoholism↗

PubMedDate not supplied

Hepatic lipid profiles in miniature pigs after alcohol feeding.

Changes in lipid profiles have not been reported for the known increases in total lipid content in livers of alcoholics. We have reported a lowering of the beta-oxidative capacity of alcoholic livers, and therefore would expect a lower turnover of fatty acids in these livers, and thus a change in lipid profile. The percentage composition of saturated and unsaturated fatty acids in the liver of alcohol-fed miniature pigs versus the controls, as well as the function of distance from the main hepatic vein, have both been determined in this study involving the feeding of ethanol for one year. Livers of alcohol-fed miniature pigs contained more total lipids than those of controls. Results also indicated significantly higher percentages of free fatty acids and triglycerides in the alcohol-fed miniature pigs, and also an increase in percentage total neutral lipids. The effect of distance from the main blood source (and therefore oxygenation) gave a fatty acid profile that showed an increase in the ratio of saturated to unsaturated fatty acids with increasing distance from the right hepatic vein. This change in ratio was independent of alcohol feeding.

Animals↗

PubMedDate not supplied