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J L Molinari

Publications and source records attributed to J L Molinari.

35 records · Page 2Linked to original sources
PubMed1983

Taenia solium: immunity in hogs to the Cysticercus.

Protection was induced in hogs against Taenia solium cysticercosis using an immunogenic complex obtained from its larval "bladder worm" form, Cysticercus cellulosae. Immunoelectrophoresis revealed that this complex contained at least eight antigens. In immunized hogs a total of 71 (mean 11.8) cysticerci were found, whereas in the control animals 397 (mean 74.9) were found. Histopathological studies showed that more than 40% of larvae obtained from immunized hogs were completely destroyed and the others were seen in various stages of degeneration. Eosinophils and mononuclear cells were observed infiltrating the internal structures of the larvae. Intense granulomatous reactions of eosinophils, lymphocytes, macrophages, epithelioid cells, plasma cells, and fibroblasts surrounded the larvae. Larvae from control hogs were intact and surrounded by a small inflammatory reaction. The cellular response was measured by the macrophage migration inhibition test, which was higher in immunized hogs when compared with control animals, either before the infection with T. solium eggs or before slaughter. No significant difference was found in the humoral response of immunized and control hogs.

Animals↗
PubMed1983

Taenia solium: cell reactions to the larva (Cysticercus cellulosae) in naturally parasitized, immunized hogs.

In hogs naturally infected with Taenia solium larvae (i.e., Cysticercus cellulosae), we studied the host response induced by antigens obtained from the larvae. Histopathological studies of cysticerci removed after 4 and 8 weeks of immunization showed an intense inflammatory reaction surrounding the larvae. The response was greater in the 8-week specimens. A dense layer of eosinophils was in close contact with the external membrane of the bladder wall and, in several cases, the eosinophils had infiltrated this tegument. Many eosinophils were seen in the spiral canal of larvae. This infiltration by eosinophils increased with time. Preparations from the 8-week samples showed many degenerated and disrupted eosinophils whose granules were found in close contact with the outer membrane of the larval tegument and, in some cases, had entered through the broken surface of this structure. More than 90% of the larvae were found in various stages of degeneration; the rest were completely destroyed and surrounded by a mass of eosinophils. After immunization, peripheral blood eosinophilia increased to 17%, whereas the eosinophilia of the control hog was 4% throughout the study. The larval worms removed from control hogs showed intact structures, with a low degree of infiltration by eosinophils and a discrete inflammatory reaction surrounding the bladder wall of the larvae.

Animals↗
PubMed1979

Immunogenic complexes obtained from Salmonella typhi-murium and Salmonella typhi Ty2 by the bacterial acetone powder method.

An immunogenic complex was isolated from Salmonella typhi-murium and another one from Salmonella typhi Ty2. Both were prepared by the bacterial acetone powder method which eliminated the cell wall, the DNA almost completely and the membrane phospholipids. The complexes were denominated "New Vaccines". The S. typhi-murium new vaccine induced, even at doses of 0.5 microgram dry weight per mouse, a high degree of protection against the challenge of the virulent microorganism. By immunoelectrophoresis, 21 antigen-antibody systems could be detected, two of them corresponding to O antigens. The S. typhi Ty2 new vaccine induced better protection than the standard vaccine (heat-phenol inactivated typhoid vaccine) when both vaccines were compared in the relative potency test. Moreover, the new vaccine had very low toxicity when inoculated in humans at doses of 1 microgram dry weight, able to elicite a high antibody titre (1/1,790 mean of 10 sera) in 75% of the tested population, estimated by the complement fixation test. In contrast, the standard vaccine induced a low antibody titre (1/222, mean of 5 sera) in 50% of the humans inoculated with 1 X 10(8) bacterial cells. The new vaccine did not induce undesirable effects whereas the standard vaccine induced an important inflammatory process in 100% of the cases, with intense local pain in 67% after 24 h post-first inoculation as well as other less severe symptoms.

Acetone↗
PubMed1976

[Ribosomal vaccine obtained from Salmonella typhimurium and tested against the challenge of an orally administered virulent microorganism].

Ribosomal preparation obtained from Salmonella typhimurium, used as a vaccine, was able to induce protection of 100% on mice challenged with 5.5 X 10(9) CFU of S. typhimirium administered by oral route. Effective dose at 50% was of 0.1085 mug, expressed as ribonucleic acid (RNA). This vaccine induced an important humoral response as soon as in nine days. The ribosomal preparation used in this work was maintained at 4 degrees C at least for eighty two days. This fact shows an adequate stability of this biological product.

Administration, Oral↗
PubMed1969

[Not Available].

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Argentina↗
PubMedDate not supplied

Immunity against the venom of Mexican scorpion Centruroides lumpidus limpidus induced by some proteins from this venom.

A protein fraction, which consisted of at least 12 proteins, was obtained from the venom of Mexican scorpion Centruroides limpidus limpidus. The molecular weights of these proteins ranged between 9,800 and 163,000 daltons. This fraction was separated from the rest of the venom components, which were almost all neurotoxins, by chromatographying the venom obtained by electrical stimulation through a Sephadex G-50M column. This fraction was non-toxic for mice, even at dose of 200 micrograms/mouse. The most important is that it was able to induce immunity against C. l. limpidus venom, since 92.8% of the animals inoculated with three doses survived after the challenge with 39.2 micrograms of venom (2 DL50 for mice of 20 g); on the contrary, 88 min after the challenge, 100% of the control mice had already died. In another experiment, this immunogen was inoculated into mice three times at variable doses. Seven days after the last injection, each mouse was challenged with 19.6 micrograms of venom. In all controls the typical envenomation picture produced by scorpion venom was developed, and death was registered in 19% of the animals. In contrast, 87% of mice immunized with the highest dose failed to show signs of envenomation or died throughout the observation time. Only two immunized animals (13%) showed mild tachycardia and hyperpnea at 120 min post-challenge. Immunoelectrophoresis and immunodiffusion tests revealed that these proteins induced antibodies against components of the most toxic fraction.

Animals↗
PubMedDate not supplied

Ribonucleic acid-protein purified from Salmonella typhi involved in experimental immunity.

An immunogenic complex was obtained from Salmonella typhi by the bacterial acetone powder method. This complex induced in mice a high degree of protection against a challenge with the virulent Salmonella. This immunogenic complex was fractionated at least into 19 fractions when chromatographied on a DEAE-cellulose column. By SDS-polyacrylamide gel electrophoresis, 25 protein bands were observed. Eleven DEAE-cellulose fractions were tested in order to know their immunogenicity. Mice were inoculated with 10 micrograms of protein of each fraction. Seven days after, the mice received a booster. Thirty days after the first inoculation, the animals were challenged with S. typhi resuspended in chondroitin-sulphate at 13%, by the intraperitoneal route. Appropriate control mice were included; 30 min before the challenge, mice had been inoculated with 850 microgram of lead acetate by the intravenous route. The immunogenic complex protected 100% of mice; six of its fractions were good immunogens; one of them, the fraction 4, was shown to contain at least 3 proteins by electrophoresis assay. This fraction induced in mice a high degree of protection against the challenge by the virulent Salmonella. Finally, a ribonucleoprotein purified from this fraction was highly immunogenic to mice against the challenge by 10 LD50 of S. typhi (1 LD50 was equivalent to 2 X 10(6) CFU).

Animals↗