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Biomedical subjects

J L Melnick

Publications and source records attributed to J L Melnick.

At least 289 records · Page 16Linked to original sources
PubMed1972

Antibodies to surface antigens of herpesvirus type 1- and type 2-infected cells among women with cervical cancer and control women.

Cells infected with herpesvirus type 1 or type 2 develop surface antigens which can be detected by immunofluorescence. Using the indirect immunofluorescence technique, we determined antibody titers to the surface antigens in 20 sera from persons with known type 1 virus infections, 20 sera from persons with known type 2 virus infections, 30 sera from women with cervical cancer, and 30 sera from matched control women. There was a good correlation between antibody activity to the surface antigens and neutralizing antibody activity for both herpesvirus type 1 and herpesvirus type 2 in all groups of sera examined. Women with cervical cancer did not have unusually high or low titers of antibody activity to the surface antigens. In the selected sera examined, analysis of antibodies to the surface antigens was not superior to the microneutralization test in distinguishing women with cervical cancer from control women.

Animals↗
PubMed1972

Detection of protein contaminants in biological preparations by discontinuous counterimmunoelectrophoresis.

Acidic proteins (serum albumin through beta(2) globulin) can be rapidly detected by the discontinuous counterimmunoelectrophoresis method in which the protein under test is allowed to react electrophoretically with specific antiserum. In the test, acidic proteins move towards the anode and cross over antibodies moving towards the cathode. The method is several thousand times more sensitive than the Lowry test, for it can detect picogram amounts of protein. With this method, foreign proteins (chicken allantoic fluids or bovine serum) were detected in virus vaccines manufactured for human use and in commercial "monospecific" immunological reagents.

Allantoin↗
PubMed1972

Biophysical and biochemical heterogeneity of purified hepatitis B antigen.

Hepatitis B antigen of the D (a+, d+, y-) subtype was purified from plasma of apparently healthy persons and from hepatitis patients. The original samples contained 20- and 42-nm particles and tubular forms (20-nm diameter). Ultracentrifugation during the purification procedure yielded pellets which were then treated at pH 2.4. Both the large, 42-nm Dane particles and the tubular forms were lost during the acid treatment of the pelleted particles, yielding a preparation containing a mixture of particles approximately 20 and 25 nm in diameter. This difference in size was substantiated in that two distinct molecular weights were calculated from high-speed equilibrium data, 3.6 x 10(6) and 4.5 x 10(6). Further heterogeneity was observed in that hepatitis B antigenic activity was present in purified particles with an isoelectric pH of 4.0 and also in those with a pH of 4.4. No significant differences were observed in the gross amino acid composition of purified antigen obtained from plasma of three different persons. (125)I-labeled, purified antigen was found to contain six distinct polypeptides with molecular weights ranging from 10,000 to 39,000.

Amino Acids↗
PubMed1972

WHO collaborative studies on poliovirus type 3 strains isolated during the 1968 poliomyelitis epidemic in Poland.

In 1968 in Poland an extensive outbreak of poliomyelitis, caused by type 3 poliovirus, began about four months after small vaccine trials with the Leon 12a(1)b (Sabin) and USOL-D bac vaccine strains had been carried out. Because of the temporal association, and because the first cases appeared in the province in which the USOL-D vaccine trial was carried out, a detailed investigation of the strains isolated from cases in the epidemic was made in four laboratories in an attempt to determine whether they were related to the two vaccine strains or to a "wild" strain. All the studies were made under code. The rct marker was of no help in determining the relationship of the epidemic strains to the vaccine strains. The McBride test and the elution marker test clearly separated the Leon 12a(1)b strains from those from the cases, but were incapable of detecting whether the epidemic strains were related to the USOL-D bac strain or to wild type 3 strains. Thus the studies did not provide valid information on the origin of the epidemic.

Child↗
PubMed1972

Hepatitis virology.

Explore the source record for details and available documents.

Antibodies, Viral↗
PubMed1971

Nonencapsidated infectious DNA of adeno-satellite virus in cells coinfected with herpesvirus.

Adeno-associated satellite viruses produce antigen detectable by immunofluorescence but not infectious virus in tissue culture cells coinfected with herpes simplex virus. Analysis of DNA extracts from these infected cells shows that large amounts of infectious satellite virus DNA are produced but not encapsidated in the system. This result indicates that satellite virus may be defective at the maturation step.

Animals↗