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Biomedical subjects

J L Harris

Publications and source records attributed to J L Harris.

69 records · Page 4Linked to original sources
PubMed1975

Scanning electron microscopy of surface and internal features of developing perithecia of Neurospora crassa.

Stages in the development of perithecia of Neurospora crassa, designated by the time elapsed after crossing, were investigated with the scanning electron microscope, from protoperithecia through perithecia. The usual examination of external features of whole specimens with this instrument was augmented by a freeze-fracture technique which allowed the viewing of development internally as well. Rapid increases in perithecial size soon after crossing were followed by the appearance, in section, of a centrum, at first undifferentiated but subsequently developing ascogenous hyphae. The perithecial beak appeared as a compact mass easily distinguishable in whole specimens from the surrounding hyphae by means of texture as well as shape. Two ascospores were photographed during emergence from an ostiole, but ostioles were found more frequently closed than open.

Cell Wall↗
PubMed1974

Scanning electron microscopy of Thermoplasma acidophilum.

The scanning electron microscope was utilized to observe the morphology of the thermophilic, acidophilic mycoplasma, Thermoplasma acidophilum. Upon examination of the surface morphology, the size and shape of this unusual mycoplasma revealed its similarity to the other mycoplasmas that have been investigated.

Agar↗
PubMedDate not supplied

Developing a protocol to prevent and treat pediatric central venous catheter occlusions.

Catheter occlusions are a common occurrence in pediatric patients with central venous catheters. These occlusions are attributable to many factors, such as mechanical problems caused by catheter and patient size, clot formation caused by blood product administration and laboratory sampling, drug precipitation, and lipid residues. Because of the significant patient risk and cost involved in replacing occluded central venous catheters, a multidisciplinary team used a quality improvement approach to determine the effectiveness of urokinase in pediatric patients. Data from the project enabled development of a decision tree for urokinase use. Statistics from the pilot test revealed that 85% of the catheter occlusions were related to thrombotic events, and urokinase was effective in all cases. However, 15% of central line occlusions were related to medication precipitates and were not effectively cleared with urokinase. Given the evidence that urokinase is effective only on fibrin-related occlusions, the decision tree was modified to improve success with occlusions caused by medication precipitates.

Catheterization, Central Venous↗