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Biomedical subjects

J L Harden

Publications and source records attributed to J L Harden.

10 recordsLinked to original sources
PubMed2006

Microscopic dynamics of recovery in sheared depletion gels.

We report x-ray photon correlation spectroscopy and diffusing wave spectroscopy studies of depletion gels formed from nanoscale silica colloids in solutions of nonabsorbing polymer following the cessation of shear. The two techniques provide a quantitatively coherent picture of the dynamics as ballistic or convective motion of colloidal clusters whose internal motion is arrested. While the dynamics possesses features characteristic of nonergodic soft solids, including a relaxation time that grows linearly with the time since shear, comparison with behavior of quenched supercooled liquids indicates that this evolution is not directly related to traditional aging phenomena in glasses.

Journal Article↗
PubMed2006

Cost savings and quality improvement--single-use suture instruments?

AIM: To determine if the instruments found in single-use suture kits are of satisfactory quality when compared with re-useable instruments and to determine the cost implications of changing to these kits. METHODS: Audit of established practice, followed by trial of new suture kits and their introduction to the department. The new practice was then audited. A cost analysis was conducted. RESULTS: The audit showed numerous problems with the traditional suture kits (instruments were breaking or no longer suitable for suturing wounds). A trial of single-use instruments demonstrated them to be high quality and provided new instruments each time. A repeat audit at one year post-introduction demonstrated no identifiable problem with the new suture kits. The subjective impression of staff was of an improvement compared to the old kits. Costs of sterilising suture instruments were determined and it was found that single use suture kits were cost effective. Assuming an average usage of 150 kits per month, at pound 4.45 each cost for sterilisation, and a total cost of pound 3.05 each for a single-use suture kit plus dressing pack per patient, this yielded a projected cost saving of pound 2520.00 per annum. The actual cost saving was pound 1981.00 that year. The shortfall was due to overestimation of average usage. CONCLUSION: Single use instruments would appear to be safe and cost effective in the emergency department setting.

Cost Savings↗
PubMed2005

Phase behavior and local dynamics of concentrated triblock copolymer micelles.

We report a neutron-scattering study to characterize the ordering and local dynamics of spherical micelles formed by the triblock copolymer polyethylene oxide (PEO)--polypropylene oxide (PPO)--polyethylene oxide (Pluronic) in aqueous solution. The study focuses on two Pluronic species, F68 and F108, that have the same weight fraction of PEO but that differ in chain length by approximately a factor of 2. At sufficiently high concentration, both species undergo a sequence of phase changes with increasing temperature from dissolved chains to micelles with liquid-like order to a cubic crystal phase and finally back to a micelle liquid phase. A comparison of the phase diagrams constructed from small-angle neutron scattering indicates that crystallization is suppressed for shorter chain micelles due to fluctuation effects. The intermediate scattering function I(Q,t)I(Q,0) determined by neutron spin echo displays a line shape with two distinct relaxations. Comparisons between I(Q,t)I(Q,0) for fully hydrogenated F68 chains in D2O and for F68 with deuterated PEO blocks reveal that the slower relaxation corresponds to Rouse modes of the PPO segments in the concentrated micelle cores. The faster relaxation is identified with longitudinal diffusive modes in the PEO corona characteristic of a polymer brush.

Journal Article↗
PubMed2005

Budding and domain shape transformations in mixed lipid films and bilayer membranes.

We study the stability and shapes of domains with spontaneous curvature in fluid films and membranes, embedded in a surrounding membrane with zero spontaneous curvature. These domains can result from the inclusion of an impurity in a fluid membrane or from phase separation within the membrane. We show that for small but finite line and surface tensions and for finite spontaneous curvatures, an equilibrium phase of protruding circular domains is obtained at low impurity concentrations. At higher concentrations, we predict a transition from circular domains, or caplets, to stripes. In both cases, we calculate the shapes of these domains within the Monge representation for the membrane shape. With increasing line tension, we show numerically that there is a budding transformation from stable protruding circular domains to spherical buds. We calculate the full phase diagram and demonstrate two triple points of, respectively, bud-flat-caplet and flat-stripe-caplet coexistence.

Biophysics↗
PubMed2004

Evolution of particle-scale dynamics in an aging clay suspension.

Multispeckle x-ray photon correlation spectroscopy was employed to characterize the slow dynamics of a suspension of highly charged, nanometer-sized disks. At wave vectors q corresponding to interparticle length scales, the dynamic structure factor follows a form f(q,t) approximately exp([-(t/tau)(beta)], where beta approximately 1.5. The relaxation time tau increases with the sample age t(a) approximately as tau approximately t(1.8)(a) and decreases with q as tau approximately q(-1). Such behavior is consistent with models that describe the dynamics in disordered elastic media in terms of strain from random, local structural rearrangements. The measured amplitude of f(q,t) varies with q in a manner that implies caged particle motion. The decrease in the range of this motion and an increase in suspension conductivity with increasing t(a) indicate a growth in interparticle repulsion as the mechanism for internal stress development implied by these models.

Aluminum Silicates↗
PubMed2003

Aging processes and scale dependence in soft glassy colloidal suspensions.

The aging behavior of colloidal suspensions of laponite, a model synthetic clay, is investigated using light scattering techniques. In order to measure the complete dynamic structure factor as a function of time and of wave vector, we have developed an original optical setup using a multispeckle technique for simple light scattering. We have thus measured the correlation of the scattered light intensity as a function of the age of the sample t(w) for various concentrations. For sufficiently concentrated samples, we observe a two-stage relaxation process. The fast relaxation is diffusive, stationary, and reminiscent of the liquidlike behavior observed in less concentrated samples. The slow relaxation behavior, however, is more complex. It exhibits two successive regimes as the sample ages. In the first regime, the decay time tau(a) increases exponentially with t(w) as long as tau(a)<t(w). In the second one, "full aging" is observed in which tau(a) is proportional to t(w). In this second regime, the relaxation of concentration fluctuations are hyperdiffusive, scaling as exp[-(t/tau(a))(beta)] with beta=1.35+/-0.15. In addition, the spatial dependence of this relaxation time scales as tau(a)(q) approximately q(-x) with x approximately 1.3.

Journal Article↗
PubMed2001

Thalidomide is distributed into human semen after oral dosing.

As part of a double-blind placebo-controlled study of the effect of thalidomide on body weight and the viral load of human immunodeficiency virus-seropositive patients, plasma and semen samples were analyzed for the presence of thalidomide. Patients were orally dosed with 100 mg of thalidomide/day for 8 weeks. Blood samples were obtained at baseline and weeks 4, 8, and 12, and semen was obtained at baseline and weeks 4 and 8. Samples were extracted with solid-phase cartridges and analyzed by liquid chromatography/tandem mass spectrometry using atmospheric pressure chemical ionization in the negative ion mode. Two of four patients taking thalidomide were able to provide semen samples. Both had detectable levels of thalidomide in their plasma (10-350 ng/ml) and semen (10-250 ng/g) at weeks 4 and 8. There was an apparent correlation between plasma and semen levels. Semen levels could be significantly greater for therapeutic doses of more than 100 mg/day. Since the threshold dose for birth defects and thalidomide exposure is not known, male patients are advised to use barrier contraception.

Administration, Oral↗
PubMed1998

Reversible hydrogels from self-assembling artificial proteins.

Recombinant DNA methods were used to create artificial proteins that undergo reversible gelation in response to changes in pH or temperature. The proteins consist of terminal leucine zipper domains flanking a central, flexible, water-soluble polyelectrolyte segment. Formation of coiled-coil aggregates of the terminal domains in near-neutral aqueous solutions triggers formation of a three-dimensional polymer network, with the polyelectrolyte segment retaining solvent and preventing precipitation of the chain. Dissociation of the coiled-coil aggregates through elevation of pH or temperature causes dissolution of the gel and a return to the viscous behavior that is characteristic of polymer solutions. The mild conditions under which gel formation can be controlled (near-neutral pH and near-ambient temperature) suggest that these materials have potential in bioengineering applications requiring encapsulation or controlled release of molecular and cellular species.

Amino Acid Sequence↗
PubMed1995

Femoral catheters increase risk of infection in total parenteral nutrition patients.

Central venous access for the administration of total parenteral nutrition is usually achieved via the subclavian or internal jugular veins. Although a high incidence of complications has been reported with the use of femoral catheters for central venous access, this route has been used when traditional central venous access is contraindicated. We retrospectively reviewed 171 patients who received total parenteral nutrition via a central venous triple-lumen catheter and compared the rates of infections in femoral vs nonfemoral access. A literature review was performed to identify associated complications of and appropriate indications for femoral catheter use. In the 171 patients studied, 355 triple-lumen catheters were placed; these included 331 nonfemoral catheters and 24 femoral catheters. Femoral catheters were placed in nine patients. Femoral catheters had a greater incidence of positive tips (42% vs 6.9%, p < .001) and related bacteremia (16.7% vs 1.8%, p = .002) than did nonfemoral catheters. The organisms most commonly isolated from the blood and catheter tips of both catheter access sites were methicillin-resistant Staphylococcus epidermidis and Candida. The use of femoral catheters for central venous access for total parenteral nutrition administration results in an increased risk of infectious complications.

Adult↗