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Biomedical subjects

J L Binet

Publications and source records attributed to J L Binet.

At least 127 records · Page 7Linked to original sources
PubMed1979

Identification of a pure splenic form of chronic lymphocytic leukaemia.

We have recently proposed a new staging system for chronic lymphocytic leukaemia (CLL) in which patients with isolated splenomegaly are classified into a distinct stage (stage II). Twenty-three such patients (from two institutions) have been studied without recorded death in a follow-up of 18 months to 30 years. This favourable prognosis justifies separation of these 'pure splenic forms' (SCLL) which must be distinguished from what Galton has termed prolymphocytic leukaemia (PL). This distinction can be made on the basis of three criteria: (i) Clinically, SCLL has a slow uneventful course and neither anaemia and/or thrombocytopenia: (ii) cytologically PL can be distinguished from other forms of CLL though atypical forms of CLL may be confused with the former; and (iii) the study of surface membrane immunoglobulins (SmIg) showed that while lymphocytes from most patients with both PL and SCLL bore uniform SmIg, suggesting a monoclonal B-cell proliferation, there was a major quantitative difference in that whereas PL lymphocytes had a number of antigenic sites close to that of normal lymphocytes (mean: 82 000 sites per cell), SCLL lymphocytes had a drastically reduced number of sites. It is our opinion that this is an important criterion for the differential diagnosis between PL and SCLL.

Aged↗
PubMed1979

Correlation between lymphographic grouping and anatomic and clinical stages in chronic lymphoid leukemia.

In a retrospective study of lymphograms in 55 patients with chronic lymphatic leukemia observed 42-170 months, four lymphographic groups were established according to lymph node size and morphology. In group 1, the lymph nodes were only slightly enlarged and pathologic in appearance. In group 2, the nodes were moderately enlarged and appeared reticular. In groups 3 and 4 the nodes were greatly enlarged and appeared foamy, striated, or "ghostlike" (similar to those seen in lymphoma). The correlation between the lymphographic groups and a new clinical classification (stages O-IV), which has been demonstrated to have prognostic values, was statistically significant: clinical stage O and lymphographic group 1; clinical stages III and IV and lymphographic groups 2, 3, or 4. Clinical stages 1 and II were dispersed throughout the different lymphographic groups. Although the specific prognostic value of the results of lymphography is not apparent in clinical stages O-II, it does seem to be established in stages III and IV. In these stages, the number of deaths was significantly greater in patients in lymphographic groups 3 and 4 when compared with those in lymphographic group 2.

Adult↗
PubMed1979

[Quantitative study of medullary adipocytes in marrow aplasia (author's transl)].

Quantification of adipocyte surface in relation to hematopoietic tissue was performed quantitatively with a classimat on 21 marrow biopsies from patients belonging to the Cooperative Marrow Aplasia Group. This analysis revealed two quantitative parameters, distribution and homogeneity, which permitted the classification of the biopsies into four groups. Each of these categories has been correlated with clinical and kinetic data.

Adipose Tissue↗
PubMed1979

[Quantitative study of the medullar fibrosis in 84 cases of idiopathic myeloid splenomegaly (author's transl)].

84 bone marrow biopsies were analysed by quantification methods with Classimat. The initial diagnosis was: idiopathic myelofibrosis with splenomegaly. A previous study divided the patients among three categories, according to the classification in three progressive stages of the bone marrow disease. The quantitative lecture was done after reticulin coloration, "in blind", and on several biopsies from the same patient. The results show good correlation with classical histologic methods. The quantitative measure of fibrosis is already important as soon as the first stages of the disease. Correlations with prognosis are more difficult to define.

Biopsy↗
PubMed1979

[Contribution of new methods of quantitative cytology and immunology to the understanding of lymphoproliferative syndromes].

Seventy one patients with a lymphoid hemopathy, three with agammaglobulinemia and six normal controls were investigated with regard to their blood lymphocyte membrane-associated light chains. Detection and quantitation of antigenic determinants were performed by means of peroxidase-labeled antibodies. Compared to normal controls, values found in chronic lymphocytic leukemia (CLL) were very low (tenfold decrease). The number of antigenic determinants on lymphoid cells from patients with blast crises supervening in CLL, prolymphocytic leukemia, Waldenstrom's macroglobulinemia and Burkitt cell acute leukemia were significantly higher than those seen in patients with CLL. The data obtained in this investigation through quantitative immunocytology constitutes a new parameter for the classification of lymphoid hemopathies and for an approach to their pathogenesis, in particular if the quantity of membrane immunoglobulin correlates with the stage of cell maturation.

Antibodies↗
PubMed1978

[Comparative study of the peripheral lymphocyte count in controls and in patients with chronic lymphocytic leukemias 4 hours after injection of hydrocortisone (author's transl)].

The peripheral lymphocyte count was investigated prior to and 4 h after a single intravenous injection of 400 mg of hydrocortisone (HSHC) in 23 controls and 43 patients with chronic lymphocytic leukemia. A reduction in the peripheral lymphocyte count was observed in all normal controls, the mean decrease being 51.9%, with differences according to age.

Aged↗
PubMed1978

[Automation in hematology (author's transl)].

A special meeting of the French Hematological Society, held on October 4 and 5, 1976, was devoted to automation in hematology. Six topics were discussed: description of the apparatus and discussion of the results of hemostasis (ELECTRA 600 D, COAGULOMETER, COAG A PET, THROMBOLAB and AUTO FI DADE); automation in blood group determination (Technicon auto-analyzer, Groupamatic MG 50, and picture analysis by the QTM 720); automatic differential white cell counts with presentation of and results obtained with the HEMA-LOG D, CORNING LARC, GEOMETRIC DATA, HEMATRAK and PERKIN ELMER DIFF 3 systems; the value of cell volume analysis in the study of red blood cells, platelets, normal lymphocytes, lymphocytes in chronic lymphocytic leukemia and blast cells; contribution of new picture analysis apparatus in the study of fibrosis and bone marrow insufficiency; the place of automation in laboratory management.

Autoanalysis↗
PubMed1978

[A new parameter for chronic lymphocytic leukemia : determination of the large lymphocytes by means of Hemalog D (author's transl)].

The munber of large peripheral lymphoid cells and the ratio of these large unstained cells (LUC) to the total number of peripheral lymphocytes were determined by means of the Hemalog D in 57 patients with chronic lymphocytic leukemia (CLL) and in 100 controls. While the absolute number of LUC per mm3 is simply a reflection of peripheral lymphocytosis, the ratio LUC/total lymphocyte count was shown to correlate with clinical staging. In controls, this ratio ranged from 3.2% to 11.2%. In CLL is was less than 11.2% in 43 patients and less than 11.2% in 14 patients. This latter group corresponded statistically to patients with advanced disease in our clinical staging system (stages III and IV). An increase in the LUC/total lymphocyte ratio is therefore a statistical criterion of poor prognosis.

Autoanalysis↗
PubMed1978

[An approximate solution to the integral equation by using non serial cuts (author's transl)].

The problem formulated and resolved by WICKSELL is reformulated for a new technique of quantitative cytology utilizing non-serial cuts instead of serial sectioning. With a technique borrowed from numerical analysis an approximate solution can be obtained. Theoretical results are in agreement with experimental results obtained using a mixture of red blood cells from humans and rats.

Animals↗
PubMed1978

Chronic lymphatic leukaemia: cellular effects of glucocorticoids in vitro.

Glucocorticoid receptor levels and steroid induced inhibition of nucleic acid precursors have been examined in lymphocytes from 27 patients at different stages of chronic lymphatic leukaemia. No correlation can be found between the level of glucocorticoid receptors and the stage of the disease. On the other hand, a significant difference (P less than 0.02) was found between stage O and stage III/IV patients, in terms of the in vitro effect of dexamethasone on [3H] uridine incorporation.

Adult↗
PubMed1978

[Inflammatory granuloma of the face with a malignant course. Atypical Hodgkin's disease (author's transl)].

A case of Hodgkin's disease beginning in the skin of the face with local lymph node involvement is reported. This was a lymphogranulomatous form without characteristic Reed-Sternberg cells in skin biopsy specimens, rendering the diagnosis difficult. There was a combination of granulomatous inflammation, plaques of necrosis and lesions of fibrinoid necrosis in the vessel walls. This form entered the classification of clinical Stage IV, with its associated serious prognosis.

Adult↗
PubMed1978

Ontogeny of B lymphocytes in mice. Quantification of surface membrane immunoglobulins by immunoperoxidase assay.

Surface membrane immunoglobulins (SmIg) of splenic lymphocytes were investigated by immunoperoxidase assay in newborn Swiss mice, their mothers and adult controls. The mean number of SmIg + cells in adult mice was 45-8% and the mean number of antigenic sites per positive cells was 108,500. In newborn mice during the first 7 days of life, values for both parameters were low (24-25--28-5% and 38,000-45,773 respectively) and began to rise after the 10th day to reach adult levels between the ages of 2 and 3 weeks. A peak above adult levels for the mean number of sites per cell was observed on day 21 with a subsequent drop to adult levels on day 28. Post-partum females were found to have consistently fewer sites per positive cell (69,000-86,600) during the 14 days following delivery than their adult control counterparts, though the difference was not statistically significant.

Age Factors↗
PubMed1977

Investigation of a new parameter in chronic lymphocytic leukemia: the percentage of large peripheral lymphocytes determined by the Hemalog D. Prognostic significance.

The number of large peripheral lymphoid cells and the ratio of these large unstained cells to the total number of peripheral lymphocytes was determined by means of the Hemalog D in 57 patients with chronic lymphocytic leukemia (CLL) and in 100 control subjects. Although the absolute number of large unstained cells/mm3 is simply a reflection of peripheral lymphocytosis, the ratio large unstained cells to total lymphocyte count was shown to correlate with clinical staging. In control subjects, this ratio ranged from 3.2 per cent to 11¿per cent. In those with CLL it was less than 11.2 per cent in 43 patients and greater than 11.2 per cent in 14 patients. These 14 patients corresponded statistically to patients with advanced disease in our clinical staging system (stages III and IV). An increase in the large unstained cells to total lymphocyte ratio is therefore a statistical criterion of poor prognosis.

Humans↗