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J Kucera

Publications and source records attributed to J Kucera.

At least 181 records · Page 10Linked to original sources
PubMed1983

Histological study of motor innervation to long nuclear chain intrafusal fibers in the muscle spindle of the cat.

Several muscle spindles of the cat tenuissimus muscle were cut in serial, 1-micron thick transverse sections and stained with toluidine blue in search for long nuclear chain intrafusal muscle fibers. Five complete poles of the long chain fibers were located. Each fiber pole displayed one plate-type motor ending situated in the extracapsular fiber region. The endings were supplied by myelinated motor axons that originated from intramuscular nerve fascicles containing motor axons to extrafusal muscle fibers. One of the endings was innervated by a collateral from a motor axon that supplied an extrafusal end-plate. Ultrastructurally, the long chain endings resembled extrafusal end-plates. They were more complex, in terms of prominence of sole-plate and degree of post-junctional folding, than any other intrafusal ending present in the spindles. The motor endings of the long chain fibers were assumed to be the terminals of static (fast) skeletofusimotor axons, which preferentially innervate the longest nuclear chain fibers of cat muscle spindles.

Animals↗
PubMed1983

Multiple-bag-fiber muscle spindles in tenuissimus muscles of the cat.

Over 300 complete and incomplete cat muscle spindles were examined in serial transverse sections of tenuissimus muscles in search of spindles with more than two nuclear bag intrafusal muscle fibers. Several histochemical and histological stains were used to identify the intrafusal fibers and assess their motor and sensory innervation. About 13% of the spindles contained either three or four bag fibers rather than the usual two. Every multiple-bag-fiber spindle possessed at least one nuclear bag1 and one nuclear bag2 fiber. The supernumerary bag fibers were either another bag1 and/or bag2 fiber, or a mixed bag fiber. The extra bag fibers had the usual morphologic and histochemical properties of cat nuclear bag fibers. All multiple-bag spindles received primary sensory innervation, and most had secondary sensory endings in addition. Their motor pattern was similar in the number, appearance and disposition of intrafusal motor endings to that of the usual two-bag-fiber spindles. Bag fibers of the same kind shared motor nerve supply in three multiple-bag spindles in which tracings of individual motor axons were obtained histologically. It is unclear whether any functional advantage is conveyed to a muscle spindle by its having more than one bag1 and one bag2 fiber.

Adenosine Triphosphatases↗
PubMed1983

Evaluation of infusion sets by biological and chemical testing.

Some of the biological and chemical tests used in toxicological evaluation of synthetic polymers were correlated. The individual tests (sperm cell motility, tissue culture inhibition, intracutaneous test, optical absorption at 220-360 nm, reducing impurities) did not mutually correspond. The final estimation of the tested device is assessed as a sum of these units. Therefore, it is necessary to evaluate the results of the individual tests in arbitrary units.

Animals↗
PubMed1983

Incidence and some ecological aspects of avian trypanosomes in Czechoslovakia.

A total of 1 874 birds belonging to 99 species was investigated on the presence of trypanosomes in Czechoslovakia, using the method of blood smears and cultivation from peripheral blood (PEN method) and autopsies with subsequent cultivation from the bone marrow. Apart from a single finding in owls, the trypanosomes were detected only in passerines which represent the majority of birds investigated (1 518 specimens belonging to 66 species). Except for sparrows, in which trypanosomes were present very rarely, they were abundant practically in all bird families represented by a sufficient number of specimens. Autopsies showed that trypanosomes occurred on the average in 23.6% of passerines. Throughout the year their presence in peripheral blood of birds ranged from a peak in June (29% of positive passerines after PEN method) to their absence in the winter months. There were differences in the incidence of trypanosomes in birds coming from localities with different biotopes. No essential difference was found in their incidence in migratory and non migratory birds. In birds several years old the incidence of trypanosomes was higher than in younger birds. Repeated investigations of ringed birds recaptured point out long-lasting infections with avian trypanosomes. A considerable correlation between the incidence of trypanosomes and that of Leucocytozoon, Haemoproteus and microfilariae indicates that avian trypanosomes are probably transmitted by blackflies (Simuliidae), biting midges (Ceratopogonidae) and louse flies (Hippoboscidae).

Animals↗
PubMed1982

A study of motor nerve terminals on cat nuclear bag1 intrafusal muscle fibers using the ChE staining technique.

Muscle spindles were traced in serial transverse sections of cat tenuissimus muscles. Histochemical staining for "myofibrillar" adenosine 5'-triphosphatase was employed to identify nuclear bag1 intrafusal muscle fibers. Staining for cholinesterases (ChE) was used to demonstrate the termination sites of motor axons along the fibers. Several types of ChE deposits could be distinguished along the bag1 fibers based on intensity of staining and morphological characteristics. Most ChE deposits could be classified as either the "pale" or the "nonpale" plates. Some ChE active areas fitted neither of these two categories. Among 328 ChE "plates" encountered on 192 bag, fiber poles, 197 (60%) were of the "pale" and 27 (8%) of the "nonpale" type with 104 (32%) remaining unclassified. These histochemical observations are discussed with regard to the current structural and functional concepts of motor innervation of the nuclear bag1 fiber. It is suggested that the histochemical (ChE staining intensity) and morphological (length and form) characteristics of bag1 fiber motor endings are not determined solely by the nature of the corresponding motor axons.

Adenosine Triphosphatases↗
PubMed1982

Morphometric studies on tenuissimus muscle spindles in the cat.

Muscle spindles were studied histochemically in serial transverse sections of 42 cat tenuissimus muscle specimens. Staining for myofibrillar adenosine triphosphatase was employed to identify nuclear bag 1, nuclear bag 2, and nuclear chain intrafusal muscle fibers. The nuclear chain fibers were further subdivided into three categories according to their polar length and the intensity of their staining for nicotinamide adenine dinucleotide tetrazolium reductase. A total of 430 spindle poles were surveyed. The mean spindle content of bag 1, bag 2, and chain fibers was established. The mean polar length of intrafusal fibers as well as that of the intracapsular and extracapsular spindle regions was determined. A cholinesterase (ChE) staining technique was used to demonstrate the termination sites of motor axons along intrafusal fibers. Two types of circumscribed ChE deposits. The "rim" and the "plate," occurred on the fibers. The nuclear chain fibers usually carried both the ChE rims and plates, while most nuclear fibers displayed only the plates. The ChE plates were assessed in term of their appearance, staining intensity, length, and location along the fibers. The mean number of ChE plates found along the fibers was established for each of the various intrafusal fiber types. These histochemical observations are discussed with regard to the current concepts of cat spindle morphology and motor innervation. The results suggest a degree of predictability in the spindle fiber content and in the distribution of motor nerve terminals along intrafusal muscle fibers, at least in the tenuissimus muscle.

Animals↗
PubMed1982

Histological study of an unusual cat muscle spindle deficient in motor innervation.

An unusual muscle spindle innervated by only one somatic motor axon and one primary sensory axon was encountered in a cat tenuissimus muscle cut in serial transverse sections and examined by light and electron microscopes. The motor axon branched to supply the distal poles of the nuclear bag2 and nuclear chain intrafusal muscle fibers through motor terminals of several types. The proximal poles of the bag2 and chain fibers and both poles of the nuclear bag1 fiber were devoid of motor endings. In spite of the limited motor nerve supply of the bag 1, bag2 and chain fibers exhibited the usual morphologic characteristics in terms of the appearance of the equatorial region, relative fiber lengths and diameters, and the number of associated elastic fibers. It appears that motor axons may play only a limited role, if any, in the differentiation and maintenance of the three types of intrafusal muscle fiber in the cat.

Animals↗
PubMed1982

A study of sensory innervation to long nuclear chain intrafusal fibers in the cat muscle spindle.

The sensory innervation of 46 poles of long chain intrafusal muscle fibers was studied histochemically by staining for NADH-TR in periodic, 8 micron thick transverse sections of cat muscle spindles. Each long chain fiber carried terminals of the primary sensory axon, and 23 of the fiber poles also displayed secondary sensory endings. With the NADH-TR reaction there was no apparent difference in the cross-sectional appearance of sensory endings on the long chain and on other nuclear chain fibers. However, the contact area between the secondary endings and the muscle fiber tended to be shorter on the long chain than on the neighboring chain fibers of shorter polar length. This was also the case for one long chain fiber in which the sensory innervation was examined in serial, 1 micron thick sections stained with toluidine blue. Discharges of the secondary sensory axons in cat spindles may be affected more by contraction of the shorter nuclear chain fibers than by activation of the long chain fibers.

Animals↗
PubMed1982

Examination of chronically de-efferented cat muscle spindles for cholinesterase activity.

Cat tenuissmus muscles were deprived of motor nerve supply for three months by sectioning of the appropriate ventral spinal roots. Muscle spindles were located in the chronically de-efferented muscles and examined histochemically in serial transverse sections. Staining for nicotinamide adenine dinucleotide tetrazolium reductase showed that the spindle sensory innervation was preserved. The de-efferented intrafusal muscle fibers retained their differential staining with the reaction for myosin adenosine triphosphatase. However, all cholinesterase-active areas that are normally found along nuclear bag and nuclear chain intrafusal fibers demonstrated loss of the enzyme activity in the chronically de-efferented spindles. It is concluded that all histochemically demonstrable cholinesterase activity within the cat muscle spindle is dependent upon the continuous presence of motor innervation.

Acetylcholinesterase↗
PubMed1982

Comparison of motor endings of the cat's muscle spindle stained for NADH-tetrazolium reductase and cholinesterase.

Cat muscle spindles were examined histochemically in serial transverse sections of tenuissimus muscles stained for ATPase, NADH-TR and ChE alternating sequentially. Motor nerve terminals on nuclear bag1, bag2 and nuclear chain intrafusal muscle fibers were identified in periodic sections stained for ChE. Intrafusal fiber regions that carried ChE-active areas were then examined in staining for NADH-TR. The motor endings on the three types of intrafusal fiber differed in their apparent histochemical content of both ChE and NADH-TR. The observations suggest that functional differences may exist among motor nerve terminals on the various intrafusal fiber types.

Animals↗
PubMed1982

The topography of long nuclear chain intrafusal fibers in the cat muscle spindle.

Muscle spindles were studied histochemically in serial transverse sections of specimens of the cat tenuissimus muscle. The nuclear chain intrafusal muscle fibers were separated into three subtypes, called long, intermediate and typical. The long chain and intermediate chain fibers tended to assume a particular position within the axial bundle of intrafusal fibers. The fibers were usually located in that layer of chain fibers that was positioned farthest away from the bag2 fiber. Furthermore, they were usually situated adjacent to the bag1 fiber throughout much of the extent of the spindle pole. Some long chain and intermediate chain fibers had several fiber nuclei abreast at the equator rather than a single row of central nuclei, as in most nuclear chain fibers. The relative position of intrafusal fibers within the cat spindle may reflect their order of formation during development, with the fibers retaining, to a variable degree, their association with the bag2 fiber which acted as template. Thus, the axial position of long chain and intermediate chain fibers suggests that they are among the first nuclear chain fibers to form. This may play a role in the known preferential innervation of these chain fibers by skeleto-fusimotor axons.

Adenosine Triphosphatases↗
PubMed1982

Appearance of sensory nerve terminals in cat muscle spindles stained for NADH-tetrazolium reductase.

Cat muscle spindles were studied histochemically in serial transverse sections of the tenuissimus muscle stained for myofibrillar ATPase, cholinesterase or NADH-tetrazolium reductase. The terminal sites of the primary and secondary axons on intrafusal muscle fibers could be demonstrated due to their high NADH-TR activity. This sensory NADH-TR reactivity at the equator and in the juxtaequatorial regions disappeared following spindle chronic de-afferentation, but not after de-efferentation. Spindle poles that carried both primary and secondary sensory endings had a longer periaxial fluid space than poles with primary endings only, and their motor innervation, as determined by staining for ChE, was positioned at the greater distance from the equator. Some of the secondary endings occurred in intrafusal regions that displayed surface fiber ChE activity. The histochemical reaction for NADH-TR represents a simple, rapid and reliable method for studies of the distribution of sensory nerve terminals in the spindle.

Animals↗
PubMed1982

One-bag-fiber muscle spindles in tenuissimus muscles of the cat.

Over 150 complete and 139 incomplete single muscle spindles were examined in serial transverse sections of cat tenuissimus muscles in search for spindles lacking one of the two types of nuclear bag intrafusal fiber. Several histochemical reactions were used to type the intrafusal muscle fibers and assess the spindle motor and sensory innervation. One complete spindle lacked a bag1 fiber, and another spindle lacked a bag2 fiber. Several incomplete spindles also lacked bag1 fibers. In addition, ten double tandem spindles contained one capsular unit each that lacked the bag1 fiber, and one triple tandem spindle had two such capsules. All one-bag-fiber spindles had primary sensory innervation, but none had secondary sensory innervation. Their motor innervation was similar to that of the usual two-bag-fiber spindles in the number and disposition of intrafusal motor endings. It is unclear whether the one-bag fiber spindles, either single or tandem-linked, are products of an aberrant spindle development or represent a true anatomical and functional subcategory of the cat muscle spindle.

Adenosine Triphosphatases↗