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Biomedical subjects

J Koudelka

Publications and source records attributed to J Koudelka.

At least 37 records · Page 2Linked to original sources
PubMed1990

[Chronic appendicitis in children].

Starting from 1976 to 1983, a total of 114 children was submitted to surgery for chronic appendicitis. The 5 to 13 years postappendectomy results were evaluated in 105 patients. From them, 68 (64.8%) were without complaints, 27 (25.7%) showed the substantial improvement. Of any favourable effect were 3 postappendectomy children, i.e. 2.8%. In one case, a serious postoperative complication occurred, this resulting in the small intestine strangulative ileus 2 mos. after the appendectomy. Different and even contradictory views are discussed in regard with the properly existence, signs, diagnosis and management of chronic appendicitis. The authors estimate chronic appendicitis to be the clinical and by no means the pathologico-anatomical diagnosis. According to them, the children are indicated to the elective appendectomy after preliminary investigation which is focused on the exclusion of other serious diseases similar in subjective complaints.

Adolescent↗
PubMed1989

[Ileus in an infant caused by a ventriculoperitoneal shunt].

The authors describe a case of adhesive ileus caused by the intraperitoneal part of a ventriculoperitoneal shunt which was performed on account of congenital hydrocephalus. Preserving the patency of the ventriculoperitoneal drainage, the ileus was treated according to principles of abdominal surgery. The functional catheter was left in situ. Complete intestinal occlusion is one of the very rare intraabdominal complications of ventriculoperitoneal shunt. In the discussion the authors analyze available data from the literature on intestinal occlusion with the above aetiology.

Cerebrospinal Fluid Shunts↗
PubMed1989

[Acalculous gangrenous cholecystitis in a toddler].

The authors describe a case of acute gangrenous cholecystitis in a 17-month-old boy manifested by symptoms of an inflammatory acute abdomen after several days of a febrile condition associated with diarrhoea. Cholecystectomy was performed and after antibiotic and infusion therapy a noncomplicated postoperative development followed. Five years after operation the boy has no subjective complaints. In the discussion the authors analyze the incidence, aetiopathogenesis, clinical, laboratory and X-ray findings of this rare disease. For the therapy of advanced cholecystitis the authors recommend cholecystectomy.

Acute Disease↗
PubMed1980

[Microbiological quality of dried milk products for child nutrition in Czechoslovakia].

In the course of four years, 20 175 samples of milk powder products for baby foods were examined. On an average, 1.18% of the products did not comply with the requirements of the Czechoslovak State Standards. Total microbe number higher than required by the standard was found in 0.36% of the samples, on an average, higher counts of enterococci in 1.02%; coliform micro-organisms were found in 0.78% of the samples. Staphylococci and salmonellae were absent. Moulds were present in 34 samples (Aspergillus flavus without the production of aflatoxin).

Animals↗
PubMed1980

Interaction of DNA and deoxyribonucleoprotein with methylene blue. Influence of acid fixation and pretreatment used in chromosome G-banding techniques.

Influence of fixation and so-called pretreatment procedures used in visualization of chromosome G-bands on staining capacity of isolated biopolymers in solution and on chromatin of cell nuclei was investigated. Whereas acid fixation as well as pretreatment procedures generally decreased the maximum binding capacity of isolated DNA and DNP for methylene blue, the dye content in acid-fixed nuclei was increased as compared with chromatin of neutral-fixed and non-fixed nuclei. The role of dehistonization and aggregation in an acid medium on the dye-binding properties of these two types of DNP was examined and the relation of higher-order structure of chromatin to its staining properties as reflected in G-banding pattern was discussed.

Acids↗
PubMed1978

Interaction of phenosafranine with nucleic acids and model polyphosphates. I. Self-aggregation and complex formation with inorganic polyphosphates.

Aggregation or phenosafranine in concentrated aqueous solutions and its interaction with polyphosphates was Studied by absorption and fluorescence spectroscopy. At concentrations > 10(-3) M phenosafranine forms dimers (Kd = 3.8 x 10(2) l.mole(-1)), which are characterized by a hypsochromic shift of the visible and near ultraviolet absorption maxima accompanied by a hypochromic effect. No fluorescence could be detected from phenosafranine dimers. Analogous spectral changes were observed when a polyphosphate was titrated with phenusafranine, which indicated that with increasing saturation of the polyphosphate binding sites phenosafranine gradually became bound in the aggregated form. Full saturation of the polyphosphate binding sites with phenosafranine was reached only when an excess of free dye was present. The cooperative binding of phenosafranine to a polyphosphate could be evaluated by means of a theory proposed by Schwarz et al. At the zero ionic strength and at 25 degrees C the binding was characterized by cooperative binding constant K = 6.2 x 10(5) l.mole(-1), number of binding sites per monomeric phosphate residue g = 0.4, and cooperativity parameter q reverse similar 30. Spectroscopic properties of phenosafranine in the aggregated and poly phosphate-bound stotes were compared with those of ethidium bromide.

Journal Article↗
PubMed1978

Interaction of phenosafranine with nucleic acids and model polyphosphates. I. Self-aggregation and complex formation with inorganic polyphosphates.

Aggregation of phenosafranine in concentrated aqueous solutions and interaction with polyphosphates was studied by absorption and fluorescence spectroscopy. At concentrations greater than 10(-3)M phenosafranine forms dimers (Kd = 3.8 x 10(2) 1.mole-1), which are characterized by a hypsochromic shift of the visible and near ultraviolet absorption maxima accompanied by a hypochromic effect. No fluorescence could be detected from phenosafranine dimers. Analogues spectra changes were observed when a polyphosphate was titrated with phenosafranine, which indicated that with increasing saturation of the polyphosphate binding sites phenosafranine gradually became bound in the aggregated form. Full saturation of the polyphosphate binding sites with phenosafranine was reached only when an excess of free dye was present. The cooperative binding of phenosafranine to a polyphosphate could be evaluated by means of a theory proposed by Schwarz et al. At the zero ionic strength and at 25 degrees C the binding was characterized by cooperative binding constant K = 6.2 x 10(5) 1.mole-1, number of binding sites per monomeric phosphate residue g = 0.4, and cooperativity parameter q-30. Spectroscopic properties of phenosafranine in the aggregated and polyphosphate-bound states were compared with those of ethidium bromide.

Binding Sites↗
PubMed1976

A compact form of double-stranded RNA in solutions containing poly(ethyleneglycol).

Molecules of single-stranded ribosomal RNA and double-stranded replicative form of phage f2 RNA (dsRNA) adopt a compact form in solutions, containing sufficiently high concentrations of salt (NaCl) and polymer (PEG). However, only in the cases of native dsRNA molecules the compact particles are characterized by a regular internal structure, which accounts for the appearance of an intense positive band in CD spectra. Heating or acidification of PEG-containing solutions of dsRNA leads to the disappearance of the intense positive CD band, which results from the "destruction" of the regular internal structure of compact particles. Comparison of properties of DNA and dsRNA compact particles formed in PEG-containing water-salt solutions suggests the existence of similar mechanisms of compactization of double-stranded polynucleotides.

Circular Dichroism↗
PubMed1976

Interaction of DNA and deoxyribonucleoprotein with methylene blue after the treatment with fixation solutions used in chromosome G-banding techniques.

Maximum binding capacity of calf thymus DNA and deoxyribonucleoprotein for methylene blue was investigated. The binding capacity of native and heat-denatured solutions of the biopolymers was compared with that of the solutions incubated with fixatives used in cytology, viz. 4% formaldehyde and methanol-acetic acid (3 : 1) mixture. The binding capacity of DNA and DNP is strongly reduced after the treatment with both fixation agents. Thermal denaturation leads to a small decrease in the binding capacity of DNA, but does not influence that of DNP. These results were compared with microphotometric determination of nuclear methylene blue content in thymocyte spreads. Fixation with MeAc increased the dye content as compared with air-dried thymocytes, whereas 4% formaldehyde exhibited practically no influence. The mechanisms connected with the different staining properties after incubating with fixation agents DNA and DNP solutions on the one hand and the nuclear material of thymocyte spreads on the other hand are discussed.

Binding Sites↗
PubMed1975

Mechanism of the mutagenic action of hydroxylamine. VIII. Functional properties of the modified adenosine residues.

The action of O-methylhydroxylamine in vitro upon some amber and rII ochre mutants of T4 phage leads to a considerable increase in the frequency of reversions and conversions of ochre and opal mutants presumably due to A yields G transitions. The transitions seem to be caused by modification of the adenine bases of the genome to give N-6-methoxyadenine which has equivocal (A- and G-like) specificity. However, the functional activity of N-6-methoxyadenosine residues in the RNA polymerase system is low. We failed to detect any significant activity of these residues either as components of a template polyribonucleotide, or as nucleoside triphosphate precursors.

Adenine Nucleotides↗