Search PubMed⌕ Search

Biomedical subjects

J Kohn

Publications and source records attributed to J Kohn.

At least 37 records · Page 2Linked to original sources
PubMed1985

The use of alkaline-phosphatase conjugated second antibody for the enhancement of immunoprecipitation techniques on cellulose acetate membranes.

A technique is described using alkaline phosphatase (ALP) conjugated second antibodies on cellulose acetate membrane (CAM) for enhancement of immunoprecipitation techniques. The method is extremely sensitive, detecting as little as 0.1 mg/l of protein and has particular application in the investigation of monoclonal immunoglobulins in human serum or urine and in hybridoma supernatants or ascitic fluids.

Alkaline Phosphatase↗
PubMed1983

Antibody synthesis within the central nervous system: comparisons of CSF IgG indices and electrophoresis.

VARIOUS LABORATORIES HAVE REPORTED DIFFERING SUCCESS RATES IN THEIR ABILITY TO DETECT INTRATHECAL SYNTHESIS OF ANTIBODY WHEN COMPARING THE INDEX OF [FORMULA: see text] with electrophoretic analysis. We selected 44 patients in the borderline area of minimal and/or equivocal abnormality by IgG index. Electrophoretic analysis (on polyacrylamide gels for oligoclonal gamma globulin pattern) of parallel specimens was performed at the same time. The number of samples giving a normal index but showing oligoclonal bands varied between 34% and 43% depending on the cut-off point. The views about normal barrier functions underlying such indices are discussed with particular reference to the pathophysiology of the blood-CSF barrier.

Albumins↗
PubMed1982

Changes in serum amylase and its isoenzymes after whole body irradiation.

A study was carried out to assess the effect of total body irradiation on pancreatic and parotid isoenzymes of amylase in patients about to undergo bone-marrow transplantation who had received high-dose cyclophosphamide. Twelve patients were studied, enzyme activity being measured before and at various times after total body irradiation. Serum total amylase activity rose rapidly within 12 hours of irradiation to a maximum at 36 hours, returning to normal by six days; most of the increase was derived from salivary damage, with a much smaller pancreatic component. These results confirm that radiation produces acute changes in amylase activity, which may be of use in assessing radiation-induced damage.

Amylases↗
PubMed1982

Vitamin A and retinol-binding protein in patients with myelomatosis and cancer of epithelial origin.

Serum vitamin A and retinol-binding protein (RBP) concentrations were measured in patients comprising 53 myeloma and 28 epithelial cell cancer cases. Vitamin A levels in these patients were found to be significantly lower than those in the 30 healthy subjects, the effect being more marked in the patients with cancer of epithelial origin. The serum concentrations of retinol-binding protein (RBP) fell in parallel with vitamin A in the epithelial cancer patients, while the RBP concentrations remained unaffected in the patients with myeloma, suggesting that the underlying factor for resulting low vitamin A levels may be different in these two groups of patients.

Adult↗
PubMed1982

Serum concanavalin-A binding in rheumatoid arthritis.

A nephelometric assay of concanavalin-A binding of serum acute phase proteins (con-A binding) has been used in cross-sectional and sequential studies of disease activity in rheumatoid arthritis (RA). Con-A binding correlated well with blood viscosity, C-reactive protein, and other individual acute phase reactants in patients with active RA. Twenty-four patients were treated for 6 months with D-penicillamine and assessed clinically and seriologically. Clinical improvement was accompanied by significant falls in both C-reactive protein and con-A binding, although the serological changes did not always occur in parallel in individual patients. The advantages of this simple, cheap assay of acute phase proteins are discussed.

Adolescent↗