Search PubMed⌕ Search

Biomedical subjects

J Knobloch

Publications and source records attributed to J Knobloch.

At least 91 records · Page 5Linked to original sources
PubMed1982

Determination of malarial antibodies by means of screening and differentiating antigens.

4455 human sera with malarial antibodies were analysed with regard to their reactivity to screening antigens (Plasmodium fieldi and P. falciparum) and to differentiating antigens (P. vivax, P. ovale and P. malariae/brasilianum). Results were obtained by indirect fluorescent antibody test. The identification rate of antibodies was significantly higher with P. fieldi than with P. falciparum antigen, and was lowest for both of the screening antigens in sera with antibodies to P. ovale. The highest cross reactivity was between P. vivax and P. fieldi, the lowest between P. falciparum and P. fieldi. The majority of sera not reactive to screening antigens was reactive to P. ovale. The reactivity of sera to one of the screening antigens was higher in the group of immigrants from malaria endemic areas in comparison to European travellers, the overall pattern of results, however, was similar in both groups of patients.

Antibodies↗
PubMed1982

[CO2-dependent mutant of Salmonella typhi causing typhoid fever in two patients].

A CO2-dependent mutant of Salmonella typhi was isolated from anaerobic but not from aerobic blood-cultures of two patients with typhoid fever. Both patients diseased after return from their home country Turkey. The strains developed in a CO2-enriched atmosphere and under anaerobic but not aerobic culture conditions. This property remained stable during 20 subcultures. The fermentative and serological pattern was identical with S. typhi T2. Both strains were of lysovar E1a.

Adult↗
PubMed1982

Serodiagnosis of opisthorchis viverrini infestation by an enzyme immuno-assay.

The application of an enzyme immuno-assay is evaluated with regard to antibody reactivity to Opisthorchis viverrini antigen in 20 Laotian patients and control groups including patients with other helminthic infections as well as persons without parasitic infestation. It is concluded that the enzyme immuno-assay indicates infection by O. viverrini. In certain circumstances, which are discussed, the test may serve for valuation of treatment.

Adult↗
PubMed1982

Efficiency of serodiagnosis in amebiasis. Results obtained by four different tests in 13 458 persons with varying exposure.

Results are reported of antibodies to E. histolytica antigen obtained by enzyme immunoassay, complement fixation, indirect hemagglutination and latex agglutination in sera of persons with varying exposure to infection by E. histolytica. The data are compared to findings in non-exposed persons and in patients with amebic liver abscess. From the results it is concluded that the value of serodiagnosis in amebiasis is higher in patients, who have traveled in tropical areas for a limited period, than in residents of the tropics.

Complement Fixation Tests↗
PubMed1980

Clinical observations in 42 patients with Lassa fever.

Under continuous observation of several months, 42 patients from the eastern province of Sierra Leone, Liberia (Lofa County), and neighbouring Guinea were identified as Lassa fever cases by indirect immunofluorescent antibody technique, indicating that the disease is endemic in these areas. The clinical course varied from mild disease to severe illness with haemorrhagic disorders. The fatality rate was 14%. The occurrence of only two possible secondary cases suggests that person-to-person spread of the disease is unimportant epidemiologically. There was a wide range of patients' ages, tribes, and occupations, including a 2 months old baby and a white US citizen. Clinical, laboratory, and histopathological investigations demonstrated the panorganotropism of Lassa virus. Haematological tests in few selected haemorrhagic cases with Lassa fever did not support coagulation disorders or thrombocytopenia as causing the bleeding tendency. The histopathologic changes bear resemblance to those observed in Argentinian and Bolivian haemorrhagic fever, both being caused by viruses of the Arena group. However, Lassa virus hepatitis may be differentiated from liver lesions occurring in yellow fever, Marburg virus disease, and Ebola (Maridi) haemorrhagic fever.

Adolescent↗
PubMed1980

Autochthonous amoebic liver abscess in Germany.

A liver abscess was diagnosed by sonography and surgery in a sewer-man who had never left Germany 14 weeks after he fell into a clearing basin and swallowed sewage. The causative agent of the abscess was confirmed immunologically to be Entamoeba histolytica.

Enzyme-Linked Immunosorbent Assay↗
PubMed1978

Ultrastructure of Ebola virus particles in human liver.

Electron microscopy of tissues from two necropsies carried out in the Sudan on patients with Ebola virus infection identified virus particles in lung and spleen, but the main concentrations of Ebola particles were seen in liver sections. Viral precursor proteins and cores were found in functional liver cells, often aligned in membrane-bound aggregations. Complete virions, usually found only extracellularly, were mainly seen as long tubular forms, some without cores. Many tubular forms had 'enlarged heads' or 'spores' and some branched and torus forms were identified. The size and structure of the Ebola virus forms appear to be virtually indistinguishable from those of Marburg virus.

Female↗
PubMed1977

[Maridi haemorrhgic fever: a new viral disease (author's transl)].

A new viral disease (Maridi haemorrhagic fever) occurred in the South Sudan in 1976. It was obviously identical with an epidemic which occurred at the same time in Zaire. The virus is morpologically closely similar to the Marburg virus. During the Maridi epemic 124 of 238 patients died (52%). Characteristic symptoms were fever and headache (100%), diarrhoea (83%), retrosternal pain (82%), vomiting (68%), haemorrhages (62%), morbilliform or vesicular rash (52%). At post-mortem there were changes in liver, kidney, myocardium and lungs, similar to those in the Marburg virus disease, as were those observed in bone marrow and peripheral blood. Despite these analagous findings, the clinical course and results of immunofluorescence indicate that it is a new disease. The epidemic ended after suitable isolation measures had been taken. There was no specific treatment but in some cases convalescent plasma and interferon were tried. The disease is transmitted among humans by direct contact or by contact with blood or excreta of patients. No animal reservoir has been found. It is possible for this disease to be imported also into countries with a modorate climate.

Adolescent↗