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Biomedical subjects

J Klein

Publications and source records attributed to J Klein.

At least 811 records · Page 45Linked to original sources
PubMed1981

[Posttraumatic fat embolism of the retina (author's transl)].

Following a description of the clinical course of general fat embolism two cases of fat embolism with eye involvement seen by the authors are described. The course of the disease as well as the ophthalmoscopic picture are discussed and the condition is differentiated from traumatic angiopathy of the retina (Purtscher's disease).

Adult↗
PubMed1981

[Cryotherapy of the ciliary body in cases of secondary glaucoma with poor prognosis (author's transl)].

The authors report on the results of cyclocryotherapy of the ciliary body applied in 79 eyes with secondary glaucoma of various etiology. In 40% of the cases regulation of intraocular pressure and freedom from pain were achieved during an eleven-month follow-up. The results were remarkably favorable in cases of neovascular glaucoma (regulation of IOP in 50%) and secondary glaucoma following iridocyclitis and intraocular hemorrhages, which usually have a very poor prognosis.

Ciliary Body↗
PubMed1981

A novel type of T-T cell interaction removes the requirement for I-B region in the H-2 complex.

When tested in the in vitro T-cell proliferation assay, H-2a cells are nonresponders to lactate dehydrogenase B (LDH-B; L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) and to IgG2a myeloma protein. However, the cells can be converted into responders either by the addition to the culture of monoclonal anti-Ia.m7 antibody or by the removal from the culture of Lyt-2+ [T-lymphocyte-associated alloantigen (Lyt)-2 positive] lymphocytes. In both instances, the responsiveness can be suppressed again by the addition to the culture of monoclonal antibodies to I region-associated (Ia) molecules controlled by the I-A subregion. These data suggest that, in some H-2 haplotypes, the response to LDH-B and IgG2a is the result of interaction between the I-A and I-E subregions. The H-2a haplotype carries a responder allele at the I-A subregion but the responsiveness of H-2a cells is normally suppressed by T cells recognizing the antigen in the context of the I-E molecules. When the recognition of I-E molecules is blocked by an antiserum or when the cells capable of this recognition are removed, the H-2a cells become responders. These experiments demonstrate a nonresponder turned responder by antibody inhibition. They also demonstrate that the postulate of the I-B subregion is no longer necessary and provide additional evidence that the Ia molecules are the products of the immune response (Ir) genes.

Animals↗
PubMed1981

The mouse primed lymphocyte typing (mPLT)test. II. Detection of variant H-2 K and D molecules in the typing analysis of the class I-associated lymphocyte-stimulating (LS) determinants, using the B10.W lines.

The mouse primed lymphocyte typing (mPTL) assay, based on the sequential reactivation of specific immunocompetent, alloantigen-reactive T blast cells in secondary mixed leucocyte culture (MLC), has been utilized to define the major histocompatibility complex (MHC) Class I-associated lymphocyte-stimulating (LS) determinants. The test panel of secondary stimulating cells has been expanded to include 25 B10.W lines (mouse strains carrying MHC regions derived from wild mice), thus permitting examination of more than thirty 'independently derived' MHC haplotypes. Data obtained using mPLT cells generated in primary MLC between H-2 K- and/or DL-disparate strain combinations indicate that each allelic Class I product expresses a unique set of LS determinants recognized by alloreactive T lymphocytes. In addition, we have observed that Class I products, which are apparently serologically identical but which are encoded for by genes of independently derived haplotypes, can express mutually distinct LS determinants. These data suggest that T lymphocytes possess the capacity to distinguish between Class I antigens that are serologically similar (or even identical) and support the concept that LS determinants recognized by T cells are distinct from the majority of specificities recognized by B cells. This concept has marked importance in interpretation of data in the human system, for which there is now an attempt to correlate serology and PLT.

Animals↗
PubMed1981

H-2 antigen frequencies among wild mice from Chile.

Thirty-five wild mice (Mus musculus L.) were captured at four different localities in Chile. The mice were typed for the presence of 15 K-, 11 D-, 14 A-, and two E-region H-2 antigens, using the complement-antibody microcytotoxicity assay. The mice from the sample representing the largest locality had a characteristic antigenic profile distinguishing them from other mouse populations thus far studied. With respect to class I H-2 antigens, the profile was characterized by the presence of antigens H-2K.16, 31, 103, 19, 108, 21, 33, 26, 115, and H-2D.4, 106, 30, 32, 111, 114, and 18 (in order of decreasing antigen frequency), and by the absence of antigens H-2K.15, 17, 20, 113, 116 and H-2D.2, 9, 110, and 112. The profile was, furthermore, characterized by the relatively high frequency of antigens H-2K.16, 31, and H-2D.4 and 106. The profile of class II antigens was also unique to the Chilean population but less conspicuously so than that of the class I antigens. Analysis of antigenic associations suggested that among the 34% blank H-2K alleles there were at least two coding for relatively frequent but as yet unidentified K antigens. Similarly, among the 57% blanks at the H-2D locus there were at least two frequent alleles encoding unidentified D antigens. Analysis of genetic distances suggested similarity between South American mice and mice from coastal regions of Europe.

Alleles↗
PubMed1981

Haplotype study. linkage between HLA--A, B, C and Bf alleles: results obtained by different statistical methods.

325 HLA-A, B and Bf haplotypes obtained from family studies were investigated. In addition, 216 of these were tested for HLA-Cw2, Cw4, Cw5. Four different statistical methods were employed to study 2, 3 or 4 loci gametic associations in order to compare the results obtained by these different methods. Our results were then compared with those of other authors who employed similar methods. Piazza's method, used to identify gametic associations, was not able to show any 3 loci associations in our material. The Delta Standard Method employed in conjunction with Factorial Correspondence Analysis was found suitable for the study of chromosomal prevalence in a population.

Complement Factor B↗
PubMed1981

The histocompatibility-2 system in wild mice. XII. An immunochemical analysis of the anti-Ia antibodies in antisera produced against 13 B10.W lines.

The I-region products of 13 congenic B10.W lines carrying wild-derived H-2 haplotypes were analyzed with anti-B10.W alloantisera. The alloantisera were tested for immunoprecipitating activity with radiolabeled glycoproteins of the immunizing donors. Eleven of the 13 alloantisera immunoprecipitated glycoproteins with SDS-PAGE mobilities consistent with m.w. of 45,000 35,000, and 26,000. The 35,000 and 26,000 products were identified as the Ia antigens of the B10.W lines. The specificity of the anti-Ia antibodies in each anti-B10.W alloantiserum was assessed by panel testing and absorption analysis with a panel of 26 glycoprotein fractions that were prepared from 11 inbred lines and 15 B10.W lines. Four new Ia antigens that are only present in the B10.W lines were detected in this analysis. The I-region products of 8 of the B10.W lines were serologically indistinguishable from those found among laboratory inbred strains. The implications of these findings for I-region polymorphisms are discussed.

Absorption↗