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Biomedical subjects

J Kent

Publications and source records attributed to J Kent.

At least 91 records · Page 5Linked to original sources
PubMed1981

Serum and milk alkaline phosphatase in human lactation.

Serum and milk alkaline phosphatase (AP) values are reported for the first 16 weeks of lactation. Serum levels at all stages are elevates above normal-pregnant controls. Sequential milk AP values are reported for the first time. Heat inactivation and phenylalanine inhibition studies would suggest that serum AP in lactation is composed of bone and liver isoenzymes, but it shows an increase in the rate at which enzymatic activity is lost when frozen, indicating that lactation serum AP is largely a different isoenzyme. Electrophoresis demonstrates several AP "slow" bands of unknown origin in lactation serum.

Alkaline Phosphatase↗
PubMed1981

Combined use of the chemitrode with osmotic minipumps for experimental brain research.

This paper describes a simple-to-build minipump-chemitrode assembly which can be used for long term electrophysiological, chemical and behavioural studies. This system consists of an osmotic minipump, a cannula and an array of nine electrodes which are connected to a miniature Winchester Socket. A detailed plan for construction of both the chemitrode and the minipump-chemitrode is given together with some suggestions for possible usages in brain research(such as kindling). The osmotic minipump consists of a collapsible reservoir of flexible, impermeable material, surrounded by a sealed layer containing an osmotic agent--all of which is contained by a semipermeable membrane. Imbibed water generates hydrostatic pressure on the flexible lining of the reservoir, gradually compressing it, producing a constant flow of its contents through the delivery portal.

Animals↗
PubMed1980

A steady state and differential polarised phase fluorimetric study of the liver microsomal and mitochondrial membranes of thermally acclimated green sunfish (Lepomis cyanellus).

The liver mitochondrial and microsomal membranes of green sunfish and rat were examined by steady state polarisation and differential polarised phase fluorimetry to determine the effects of seasonal adaptation of membrane dynamic structure to temperature. Steady state polarisation studies indicated that the liver mitochondria of green sunfish acclimated to different temperatures showed a greater partial compensation of membrane fluidity for the fatty acid composition of both membrane preparations generally became more unsaturated at lower acclimation temperatures, though the differences between 5 degrees C and 25 degrees C acclimated fish were more pronounced in the mitochondrial fraction than in the microsomal fraction. Differential polarised phase fluorimetric studies indicated that the rotations of diphynylhexatriene in mitochondrial and microsomal membranes were highly hindered, though the hindrance offered by membranes of 25 degrees C acclimated green sunfish was far greater than that offered by the membranes of 5 degrees C acclimated fish, thus supporting the concept of homeoviscous adaptation. The absolute rotational rate was not consistently affected by acclimation treatment.

Acclimatization↗
PubMed1978

Hospital-based educators take a look at their jobs.

Cost containment was high on the list of concerns at the annual meeting and conference of the American Society for Health Manpower Education and Training in New Orleans in June. Speakers exhorted attendees to look at their profession more closely to be sure that they are doing all that they should be doing.

Health Education↗
PubMed1976

Mouse ovarian alkaline phosphatase activities that respond to gonadotropins: histochemical and biochemical studies.

Alkaline phosphatase activity was measured in whole ovarian homogenates from pre-pubertal mice of different ages, with and without prior injection of human chorionic gonadotropin. Alkaline phosphatase activity was also scored in the different cell types in sections of similar ovaries, using two distinct histochemical procedures. The results from those methods differed. Biochemical studies indicated the presence of three distinct alakaline phosphatase activities: I and Ib, both optimal at pH 10.4 and with similar substrate requirements and inhibitor sensitivities (phosphatase I being characteristic of unstimulated ovaries and Ib of ovaries stimulated with human luteinizing hormone or human chorionic gonadotropin), and phosphatase II, optimal at pH 9.4, with different substrate requirements and inhibitor sensitivities. The differences observed using the histochemical procedures can probably be accounted for by the effects of different incubation conditions on the activities of these three enzymes.

Aging↗
PubMed1976

Some problems concerning the assay of Clostridium chauvoei vaccine in laboratory animals.

The potency test prescribed for Cl. chauvoei vaccines in the British Veterinary Codex requires the complete survival of a group of immunized guinea pigs and death of all unvaccinated controls. Problems arising from the influence of extraneous factors such as animal strain, diet and seasonal variation on the outcome of this test are discussed together with alternative methods that might permit the use of a standard preparation.

Animals↗
PubMed1975

Coated versus non-Proplast-coated endosseous blade-vent dental implants.

In order to determine the efficacy of applying Proplast to dental endosseous bladevent implants, this material was coated on half of sixteen such implants which were bilaterally placed in the mandibles of eight rhesus monkeys. No difference in the clinical acceptability could be ascertained between the two groups. Histologic examination revealed a loose connective tissue layer around the noncoated implants which was not evident around the coated ones.

Alveolar Process↗
PubMed1975

Histochemical studies on three gonadotrophin-responsive enzymes in the infantile mouse ovary.

The activities of alkaline phosphatase, NAD diaphorase and NADP diaphorase increased in infantile mouse ovaries in response to injected gonadotrophins. The distribution and activity of these enzymes were studied in detail in the ovaries of normal mice from 1 to 41 days after birth and in mice injected at various ages with FSH, LH and HCG. Granulosa cells contained NAD and NADP diaphorases. Thecal cells contained NADP diaphorase and alkaline phosphatase with NAD diaphorase first appearing in the thecae of larger follicles 11 days after birth. All three enzymes occurred in interstitial tissue, in the interfollicular stroma and in groups of gonadotrophin-responsive cells in the medulla. These medullary cells and the interstitial tissue were stimulated by exogenous LH and HCG but not by FSH. Granulosa, theca and interfollicular tissue were stimulated at some stage by each of the three injected hormones. The normal pattern of development is discussed in relation to the changing serum levels of endogenous gonadotrophin found in similar mice. It is concluded that the enzyme changes were closely and reciprocally related to endogenous hormone concentrations.

Age Factors↗