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Biomedical subjects

J Kazda

Publications and source records attributed to J Kazda.

At least 37 records · Page 2Linked to original sources
PubMed1981

Conditions relevant to the occurrence of acid-fast bacilli in sphagnum vegetation.

Biotopes with intact sphagnum vegetation were studied in Naustdal, West Norway: the health district with the former highest leprosy incidence rates in Norway. Concentration of acid-fast bacilli and concentration of non-cultivable acid-fast bacilli found in the vegetation fluid were compared with a series of variables related to the local environment and to the samples of vegetation. In bivariate analyses high concentrations were found in biotopes with orientation toward South, with a high coverage of vascular plants and with presence of Ericaceae. High concentrations were also found where dry weight of a vegetation was high, where pH in the fluid was low and where dry weight of the fluid was high. In a multivariate analysis, log-linear model, based on concentration of acid-fast bacilli as the dependent variable and orientation, pH, dry weight of vegetation and dry weight of fluid as the independent variables, all two-factor effects including the dependent variable were retained in the model. Furthermore, orientation versus dry weight of vegetation and dry weight of fluid versus dry weight of vegetation were retained.

Bacillus↗
PubMed1980

Isolation of non-cultivable acid-fast bacilli in sphagnum and moss vegetation by foot pad technique in mice.

In the former leprosy-endemic coastal area of Norway, 122 samples of sphagnum and moss vegetation were collected from 6 biotopes and examined for non-cultivable AFB by foot pad inoculation. Of the 759 foot pads examined, 20% contained non-cultivable AFB. A significantly higher frequency was found in a habitat where Sphagnum cuspidatum was preponderant, the sphagnum species from which the maximum yield was obtained. The bacteria were polymorphous, solidly staining AFB, which multiplied in passage in foot pads while they could not be cultivated on the conventional media for mycobacteria. Efforts are continuing to identify these AFB by biochemical methods and by inoculation into nine-banded armadillos.

Animals↗
PubMed1979

Cultivable mycobacteria in sphagnum vegetation of moors in South Sweden and coastal Norway.

Intact sphagnum vegetation from moors in south Sweden and coastal areas of west Norway contained cultivable mycobacteria in 32% and 30% of the specimens, respectively. This frequency of specimens is lower than the 50% previously found in the partly altered moors of northwestern Germany, but the Scandinavian moors contained a larger variety of species. On both intact and altered moors M. chelonei and M. sphagni sp. nov. were found, the latter a homologous group of 151 strains. In south Sweden the highest frequency was found in S. balticum, S. recurvum. S. tenellum and S. compactum & molle. (40-65%). In coastal Norway the highest frequency was found in S. rubellum (48%) which offers favourable conditions for the accumulation of solar energy due to the red brown colour in the upper parts. Combined with a high humidity in coastal Norway in summer, this may contribute to the growth of mesophilic mycobacteria. A significant affinity of M. chelonei to S. tenellum was stated.

Mycobacterium↗
PubMed1978

[The behaviour of mycobacterium intracellulare serotyp Davis and mycobacterium avium in the head region of sphagnum moss vegetation after experimental inoculation (author's transl)].

After the inoculation in the head region of sphagnum moss vegetation (Sph. magellancium-Erica Association and Sph. apiculatum-Eriophorum angustifolium Ass.) reach the mycobacteria the hyaline cells of Sphagnum (Fig. 1). The count of M. intracellulare serotyp Davis decreases rapidly already in three days after the inoculation of Sph. magellanicum Ass. and this trend in the course of incubation is being continued. In 27 days following the inoculation only 2 of 7 specimens contained a small amount of this mycobacteria (Tab. 2, Fig. 2). In the head region of Sph. apiculatum Ass. the mycobacterial count was more rapidly going down. Already 13 days after the inoculation only 2 of 7 specimens were found mycobacteria-positive; 24 days after the inoculation no mycobacteria were present (Tab. 3, Fig. 3). The elimination of M. avium from the head region of Sph. magellanicum Ass. follows within 13 days after the inoculation (Tab. 4, Fig. 4). For the microbial growth well predisponated hyaline cells with their porous cell wall and hollow spaces possess probably a mechanism, which is effective against microbial contamination of the productive head region of Sphagnum vegetation. Our further investigations have shown, that the growth of mycobacteria follows in the deeper, partially decomposed region of Sphagnum vegetation (KAZDA, 1978).

Mycobacterium↗
PubMed1978

[Multiplication of mycobacteria in the gray layer of sphagnum vegetation (author's transl)].

The capillary moisture of the gray layer of the Sphagnum magellanicum ass. contains nutrients for the growth of mycobacteria. Tested in vitro significant multiplication was stated in M. intracellulare, serotyp Davis, M. avium, M. scrofulaceum, M. fortuitum, M. xenopi, M. kansasii, M. borstelense, M. flavescens, M. gaxtri, M. gordonae, M. marinum, M. terrae, M. smegmatis, M. spec. "S" and M. nonchromogenicum (Fig. 1 and 2). There was no evident multiplication in M. phlei, M. triviale, M. tuberculosis, M. bovis, and M. ulcerans in vitro. Using the cultivation of mycobacteria in the hollow fibers (pore size 100,000 mol. weight) inserted directly in the gray layer of the Sph. magellanicum ass. a significant growth of all 18 species investigated was stated (the same species as above, excl. M. bovis and M. tuberculosis; Fig. 3). A rapid multiplication was detected also in M. ulcerans. The sphagnum vegetation spreads over all continents. It develops very good under wet conditions surrounding springs and brooklets in the moutain region. Accumulation of solar heat directly under the surface of this vegetation makes possible a temperature 15-28 degrees C higher than the air temperature. In this way favorable conditions for mesophilic mycobacteria arise over an extend period of time. The close contact to water contributes to the wide distribution of mycobacteria.

Mycobacterium↗
PubMed1978

[Stimulatory action of Brassica oleracea var. sabauda (Savoy) on mycobacteria and mycobacterial infection (author's transl)].

Eleven vegetable species were investigated for the presence of growth-stimulating activity on mycobacteria. It was found that savoy juice (Brassica oleracea var. sabauda), given orally, posessed a pronounced stimulating effect on M. tuberculosis, M. bovis, M. kansasii, M. avium and M. intracellulare serotype Davis in the serum of experimental animals when tested in auxanograms (Table 1). Another 2 species, paprica (Capsicum frutescens) and radish (Raphanus sativus var. albus) showed only insignificant stimulating activity. Oral application of savoy juice in addition to ordinary diet had an aggravating effect on guinea pigs infected peritoneally with M. kansasii. Tuberculosis of the spleen was found only in the groups treated with savoy juice (in 28.57% after administration of 1 mg and 47.36% after that of 10 mg of mycobacteria). The counts for animals with tuberculous lesions of the lung and liver were significantly higher in the groups treated with savoy juice (Table 2, Fig. 1) as compared with those for infected, but untreated guinea pigs. The possible influence of stimulating substances in vegetables on mycobacterial infection, especially during malnutrition, is discussed.

Animals↗
PubMed1977

[The course of Jones-mote reaction in guinea pigs after the sensitization with mycobacteria (author's transl)].

Mycobacterial antigens induce of JONES-MOTE reaction, which is well stated in 7 to 9 days after being intracutaneously sensitized (Tab. 1). The reaction is adjuvant independent; Al(OH)3 as an adjuvant stabilizes and enlarges the reaction in the interval between 7 and 9 days after the sensitization (Tab 2). The erythema size, read 6, 24 and 48 hours after the intradermal testing shows no significant differences (Tab. 3). The maximal intensity of the JONES-MOTE reaction was found after 24 hours (Tab. 4). The sensitization with 0.2 mg of bacterial mass is sufficient (Tab. 5, Fig. 1). The size of erythema increases when 0.5 mug to 10 mug of homologous sensitin are used (Tab. 6, Fig. 2). An oral application of M. fortuitum increased the total count of basophilic leukocytes and caused cross reactions (Fig. 3).

Adjuvants, Immunologic↗
PubMed1976

[Breeding and management of mycobacteria-free guinea pigs (author's transl)].

A number of mycobacterial species are detectable under conventional holding condition of guinea pigs. These mycobacteria originating in drinking water and litter caused cross reactions in the Jones-Mote hypersensitivity test. Using suitable precautions it was possible to breed and hold the animals mycobacteria-free. The precautions depend mainly in alteration of the wire mesh floor in cages to avoide the contact of the animals with the litter, in cleaning and desinfection of water bottles, in using of heated water and food and in the prevention of mycobacterial contamination from the staff. The control examination on mycobacteria without treating is given in details. Cases are refered in which a oral rece ption of mycobacteria can alter the immune response. The modification of guinea pigs management to the mycobacteria-free ones is possible in a short time and with minimal cost.

Animals↗