Distribution of Staphylococcus species on animal skin.
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Animals↗
Biomedical subjects
J Kawano
Publications and source records attributed to J Kawano.
Penetration in vitro of newly excysted juvenile flukes of Japanese Fasciola sp. through ligated intestines of rabbits, mice, rats and chickens.
Ligated intestines of rabbits, mice, rats and chickens were used to examine the penetration of newly excysted juvenile flukes of Japanese Fasciola sp. in vitro. In rabbit intestines, the penetration rate was relatively high in the rectum and duodenum. Penetration rates in the jejunum, ileum, cecum and colon were comparable to those in the rectum and duodenum, although it was lower in the appendix. In the case of mouse, juvenile flukes penetrated the duodenum, jejunum, cecum, and rectum at considerably high rates. In rat intestine, penetration by flukes was less in the duodenum and rectum, although flukes were detected in the jejunum. In chicken intestine, flukes barely penetrated the duodenum, jejunum and rectum. Consequently, newly excysted flukes of Fasciola sp. seem to penetrate any region of the intestine in rabbits and mice. In rats, the middle small intestine may be the site suitable for flukes to penetrate. In chickens, the difficulty in penetration of the intestinal wall may be one of the reasons why chickens are scarcely infected with Fasciola sp.
Animals↗
Factors contributing to the resistance of chickens to infection with Japanese Fasciola sp.
Attempts were made to clarify the factors contributing to the resistance of chickens to infection with Japanese Fasciola sp. Infection was not successfully established in chickens by oral inoculation of metacercariae, nor by inoculation of excysted juvenile flukes into the body cavity or to the liver surface. Many metacercarial cysts were detected within two days in the feces of orally inoculated chickens. In the in vitro excystation test with chicken bile at 42 degrees C, metacercariae emerged successfully. These results indicate that the major resistant factors may not act during the migration from the mouth to the liver. Histopathological examination of the liver of experimental chickens could not prove the effect of a resistant factor. Excysted flukes were cultivated at 37-42 degrees C in RPMI1640 supplemented with calf serum, with the result that the survival rate of flukes fell with higher temperatures. When chicken serum was used instead of calf serum, flukes survived for a long period of time at 37 degrees C, while all died within four days at 42 degrees C. The higher body temperature of chickens than that of other mammalian hosts is considered to be the major factor contributing to the resistance of chickens to infection with Fasciola sp.
Animals↗
Sialomucin in middle ear cholesteatoma perimatrix.
Mucosubstance histochemistry of human middle ear cholesteatoma revealed that sialomucins are abundant and sulfomucins present in small amounts in the glandlike structures of the cholesteatoma perimatrix. Based on the study, various glycoproteins were injected into the dermis of the external ear canal and infiltrated into the tympanic membranes of guinea pigs. Tympanic membranes were obtained 7 days later and light- and electron-microscopically studied. Injection of a sialomucin from bovine submaxillary gland resulted in marked proliferation of epidermis and degeneration of the lamina propria. Asialomucin prepared from sialomucin by hydrolysis produced mild thickening of the epidermis but the lamina propria was not degenerated. Fetuin and bovine serum albumin did not cause proliferation of the epidermis. Cholesterol granuloma formed in some of the specimens injected with sialomucin. The presence of sialomucin in cholesteatoma perimatrix and these experimental studies using tympanic membranes of guinea pigs suggests that sialomucins participate in the proliferation of epidermis and degeneration of subepidermal connective tissue in human middle ear cholesteatoma.
Animals↗
Immunohistochemical localization of dipeptidyl aminopeptidase IV in thyroid papillary carcinoma.
The localization of dipeptidyl aminopeptidase IV expressed aberrantly in thyroid carcinoma was studied by immunoelectron microscopy using a monoclonal antibody to the enzyme with special reference to enzyme-histochemical staining of the enzyme. Five thyroid papillary carcinomas were investigated including two lymph-node metastases. All cases showed the dense immunoreaction product on the apical membrane and only traces of the product on lateral membranes, endoplasmic reticulum and nuclear membranes. In one case only, the dense product was observed on basal tubular structures. Analysis, using immunogold labelling on pre-embedded cryosections, revealed that dipeptidyl aminopeptidase IV was localized on the luminal surface of cancer cells. Two different distribution patterns of dipeptidyl aminopeptidase IV activity staining, diffuse and apical patterns, reported previously were thought to be due to different amounts of dipeptidyl aminopeptidase IV in the cytoplasm of cancer cells. This enzyme-histochemical staining method is useful for pathological diagnosis of thyroid tumours and can be applied to clinical materials. The enzyme localization is revealed by the staining pattern.
Adolescent↗
[Clinical problems in the treatment and physiopathology of drug-induced peptic ulcers].
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Animals↗
Glycoconjugate histochemistry of Xenopus laevis fundic gland with special reference to mucous neck cells during development.
Mucous neck cells (MNCs) of the fundic gland are phylogenetically thought to have first appeared in amphibians. We studied the origin and differentiation of MNCs in fundic glands of Xenopus laevis. By means of lectin histochemical methods using Griffonia simplicifolia agglutinin-II (GSA-II), MNCs were detected specifically in fundic glands of adult X. laevis. Mucous granules of MNCs were labeled by GSA-II-colloidal gold (CG) staining. Other cells such as surface mucous cells (SMCs), oxynticopeptic cells (OPCs), and endocrine cells did not react to GSA-II. Ulex europaeus agglutinin-I specifically stained OPCs, but not MNCs and SMCs. During the morphogenetic period of the stomach in metamorphosing larvae, GSA-II reactive cells randomly appeared in various portions of the underdeveloped fundic glands and then rapidly localized in the neck portion. At this time, newly appearing mucous granules of MNC type were labeled by GSA-II-CG. Two types of cells intermediate to MNCs and SMCs and intermediate to MNCs and OPCs were observed in the larval gastric region. Cells intermediate to MNCs and OPCs were also found in adults. In these cells, mucous granules of MNC type were labeled by GSA-II-CG, but mucous granules of SMC type and zymogen-like granules did not react to GSA-II. These observations suggest that GSA-II is a useful marker in studying the differentiation of MNCs and their precursors regardless of species differentiation.
Animals↗
[A case of postpartum fever caused by Mycoplasma hominis infection].
A 26-year-old housewife was admitted to our hospital with a history of high fever after previous cesarean delivery. She had premature rupture of the membrane on the 41st week of pregnancy and the amniotic fluid was found to be cloudy on the fourth day after rupture. Therefore, cesarean delivery was performed. On the first day of operation, her body temperature increased up to 38 degrees C in spite of the treatment with Latamoxef (LMOX), 3 g/day. A sample of intrauterine material yielded M. hominis in pure culture. After administration of Minocycline (MINO) with antimycoplasmal activity, the clinical symptoms improved by the 11th day of operation. Sera obtained after the infection showed antibodies to M. hominis in ELISA study. These results suggested that the cause of this postpartum fever was M. hominis infection.
Adult↗
Subcellular localization of N-acetylglucosaminide beta 1----4 galactosyltransferase revealed by immunoelectron microscopy.
We prepared a monoclonal antibody (MAb) against N-acetylglucosaminide beta 1----4 galactosyltransferase purified from F9 embryonal carcinoma cells. The MAb recognized the protein portion of the enzyme, since it inhibited galactosyltransferase activity, reacted with the enzyme both from F9 cells and from bovine milk, and did not exhibit anti-carbohydrate activity. Using this MAb, we studied the subcellular localization of the enzyme by immunoelectron microscopy. Intense staining was observed in trans-Golgi stacks within testicular interstitial cells and mucous neck cells, confirming the specificity of the immunological reaction. Cell surface galactosyltransferase was detected in the following regions: cultured cells such as F9 embryonal carcinoma cells, testicular interstitial cells, seminiferous tubule epithelial cells, Sertoli cells, the head of the epididymal sperm, epididymal epithelial cells, and apical surfaces of epithelial cells in the fundic gland and of intestinal goblet cells. The use of Triton X-100 intensified the cell surface immunoreactivity, and in certain cases the mode of distribution of the cell surface enzyme was different from that described in previous reports. In addition, nuclear envelopes of cultured cells were distinctly stained. The possible significance of the latter finding is discussed in relation to recent advances in nuclear localization of glycoproteins.
Animals↗
Characteristics of Staphylococcus aureus isolated from lesions of horses.
Seventy-six Staphylococcus aureus strains isolated from various lesions of horses were characterized. All of the 76 strains were identified as biotypes B (38.2%) and C (61.8%). Of 55 strains tested, 42 (76.4%) were differentiated into 7 coagulase types. Coagulase types V and VII were predominant in the metritis strains. Coagulase type II was found most frequently in the strains from phlegmon, dermatitis, sinusitis, empyema sinus, and nasal catarrh. Forty-two (55.3%) of the 76 strains were differentiated into 24 phage patterns. Twenty (58.8%) of 34 typable strains from metritis were lysed by the human group I phage 52, and group II phages 3A, 3C, 55 and 71. Forty-five (59.2%) of the 76 strains were resistant to 1 or more of 6 antibiotics. Strains resistant to penicillin G, irrespective of source, were most frequent (95.6%). Forty (93.0%) of 43 strains resistant to penicillin G alone or in combination with other antibiotics produced beta-lactamase. Only 8 (10.5%) of the 76 strains produced enterotoxins A (n = 2), B (n = 1) or C (n = 5), and they all were isolated from metritis. Only 1 strain isolated from phlegmon and 2 from metritis produced exfoliative toxin (ET) and toxic shock syndrome toxin-1 (TSST-1), respectively. The latter 2 strains also produced enterotoxin C. The results of the present study showed the first evidence of the presence of both ET- and TSST-1-producing S. aureus isolated from horses.
Animals↗
Leachability of denture-base acrylic resins in artificial saliva.
We studied the influence of salivary acidity on leachability of denture-base acrylic resins with etiological interest in denture stomatitis because denture surfaces are frequently exposed to acidic conditions in the oral cavities. Auto-, heat-, and microwave-polymerized resins were immersed in artificial saliva with pH ranging from 4.0 to 6.8 at 37 degrees C, and leachables were pursued quantitatively with time. Methyl methacrylate, methacrylic acid, and benzoic acid leached from all resins. Their concentrations in the saliva were markedly high for auto-polymerized resins, while leachability of heat- and microwave-polymerized resins was so low that quantitative analysis of leachables was impossible. Lower pH showed higher concentrations of methyl methacrylate, although no apparent association was confirmed between salivary acidity and its own leachability. The concentrations of methacrylic acid increased remarkably with an increase in pH, which was probably due to hydrolysis of methyl methacrylate. These results suggest that chemotoxic actions of auto-polymerized resins are potentially ascribable to methyl methacrylate under more acidic conditions and to methacrylic acid under less acidic conditions.
Acrylic Resins↗
Isolation of Staphylococcus species from the tonsils of healthy cattle and phage patterns of isolates.
Staphylococci were found in the tonsils of 121 (75.2%) of 161 cattle. There were 15 different species, 10 belonging to novobiocin-sensitive species. The most predominant species was S. simulans (79.3% of the 121 carriers), followed by S. aureus (20.7%), S. chromogenes (10.7%) and S. epidermidis (8.3%). The other 11 species were present in 0.8 to 5.8%. Twenty-six unidentifiable isolates were isolated from 26 (21.5%) carriers. Sixty-two (51.2%) of the 121 carriers yielded two to five Staphylococcus species together while only one species could be found in each of the other 59 (48.8%). Combinations of S. simulans and other species were most frequently encountered in 50 (41.3%) of the 121 carriers. Twenty-four (96.0%) out of 25 S. aureus isolates, 3 (42.9%) of 7 S. hyicus isolates and 45 (25.4%) of 177 coagulase-negative staphylococci (13 species and unidentifiable isolates) isolates were phage typable. Most of S. aureus isolates were lysed by bovine phages 119 (n = 16) or 116 (n = 5). Thirty-three (25.4%) of 45 coagulase-negative staphylococci typable isolates with Pulverer's phage set showed the phage pattern ph5/ph9/ph10/ph12/ph13/U4/U14/U16/++ +U20/U46. The tonsils of cattle thus appear to be a suitable environment for Staphylococcus species, particularly novobiocin-sensitive species.
Animals↗
[Studies on oral sensation in patients with implant denture. 4. Ability of hardness discrimination].
The ability of hardness discrimination in 11 implant denture wearers was compared with those in 10 bridge wearers and 11 normal dentate subjects. The hardness discrimination was tested using silicone rubber blocks having rubber hardness that ranged at intervals of 10 degrees from 30 degrees to 70 degrees. Using a rubber block with rubber hardness of 50 degrees as a standard, the ability of discriminating rubber blocks of different hardness was examined. All the three groups examined had excellent ability of discriminating the rubber blocks of rubber hardness of 30 degrees and 70 degrees from the standard block. The ability to discriminate the rubber block of rubber hardness of 40 degrees from the standard block was only slightly lower than but almost the same as the ability of discriminating the hardness of 30 degrees and 70 degrees in all the three groups. However, the ability of discriminating the rubber block of rubber hardness of 60 degrees from the standard block was significantly lower in the implant denture wearers, compared to the normal dentate subjects and bridge wearers.
Dental Implants↗
[Clinical study on the correlation between occlusion raising and free-way space].
This paper presents three cases in which CSC telescope dentures were inserted following occlusion raising. Post-operative course remained uneventful in all three cases. Follow-up observations suggested that the use of CSC telescope denture, is helpful in prosthetic treatment which includes occlusion raising. In Case 1, occlusion was raised to a level above the free-way space. However, a new free-way space appeared after occlusion raising and it existed for a long time. A free-way space, which conformed to a space calculated from the free-way space before occlusion raising and the amount of occlusion raising, appeared in Case 2. In Case 3, however, the free-way space after occlusion raising was narrower compared to a space calculated from free-way space before occlusion raising and amount of occlusion raising.
Dental Occlusion↗
In vitro adherence of microorganisms to denture base resin with different surface texture.
We examined the effects of various denture base resin surface textures on the adherence of microorganisms. S. sanguis and B. gingivalis adhered in greater amounts to the denture base resin than the other microorganisms tested. As to bacterial adherence according to polishing state, S. oralis, B. gingivalis C-101, and B. intermedius C-001 more adhered to the No. 400 paper-polished surface than to the buff-polished and smoothening-treated surfaces. S. sanguis less adhered to the smoothening-treated surface. S. mitis and C. albicans, on the other hand, more adhered to the smoothening-treated surface. For the other microorganisms tested, no relationship was observed between surface texture and bacterial adherence. The fall-off test revealed no remarkable differences in the fall-off of S. sanguis and B. gingivalis C-101 by the types of surface treatment. However, the fall-off of C. albicans was poorest from the No. 400 paper-polished surface. These results indicate that smoothening the denture base surface is important for denture plaque control.
Bacterial Adhesion↗
[Radiographic evaluation of 12% Au-Ag-Pd alloy cast lower denture plates constructed by dental student as a laboratory exercise].
We evaluated 12% Au-Ag-Pd alloy cast lower denture plates prepared by dental students in laboratory exercises for casting defects. First, we determined the most suitable exposure conditions for nondestructive radiographic test of 12% Au-Ag-Pd alloy. Subsequently, 130 plates were nondestructively evaluated by radiography under the most suitable exposure conditions. In radiographic testing using X-Omat RP films (Eastman Kodak Co.) and X-Omatic Fine Screen (Eastman Kodak Co.), the most suitable exposure conditions involved a target-to-film distance of 1.9 m, tube voltage of 148 Kvp, tube current of 10 mA, and exposure time of 1.5 sec. Preliminary radiographic examination of artificial casting defects revealed semispherical defects in the diameter of 0.25 mm in 1.6 mm-thick 12% Au-Ag-Pd alloy plates and detects of 0.26 mm in 2.4 mm thick plates. In the 130 cast plates, 56 casting defects were detected. These defects were found at the left webbing part in 40 (30.8%), right webbing part in 36 (27.7%), lingual bar in 7 (5.4%), I-bar in 4 (3.0%), and at other regions of the metal frames in 3 (2.3%) cases. These results indicate that nondestructive radiographic testing is as effective in detection of casting defects in 12% Au-Ag-Pd alloy cast plates as it is in Co-Cr and Ni-Cr alloy plates. This method was also found to be effective in evaluation of the practice metal frames constructed by dental students.
Dental Casting Technique↗
[Clinico-statistical observation of various prosthetic restorations at the Prosthetic Department, Asahi University Hospital. 3. Frequency of various prosthetic restorations inserted for the duration from April 1986 to March 1987].
The statistics for prosthetic restorations used at the Prosthetic Department of Asahi University Hospital from April 1986 to March 1987 were compared with those obtained in earlier period (April 1983 to March 1984). A total of 2,360 prosthetic restorations were used. This was a 12.5% decrease compared to the previous period. No significant sex-related difference was found in the number of restorations used. The number of prosthetic restorations was decreased in patients in their forties who had the most restorations in the previous period. During the period investigated in this study, the number of prosthetic restorations was highest in the patients in their fifties and lowest in those in their eighties. By sex, prosthetic restorations were used most frequently in males in their fifties and in females in their thirties. The prosthetic restorations used during this period had almost the same proportions as in the previous period. Most frequently used were full cast crowns followed by facing crowns, as were during the previous period. The former amounted to 36.1% and the latter to 22.1%. Full dentures were the least used restorations and amounted to 5.2% of all the prostheses. The use of facing crowns had considerably decreased in patients in their forties, although this age group had the highest frequency of use during the previous period. Full cast crowns were most frequently used in patients in their thirties, removable partial dentures in those in their fifties and total dentures in those in their seventies.
Adolescent↗
[Clinico-statistical observation of various prosthetic restorations at the Prosthetic Department, Asahi University Hospital. 4. Circumstances concerning prosthetic method for missing teeth for the duration from April 1986 to March 1987].
The statistics for prosthetic restorations used for missing teeth at the Prosthetic Department of Asahi University Hospital from April 1986 to March 1987 were compared with those obtained for an earlier period (April 1983 to March 1984). Fixed bridges and removable partial dentures constituted the majority of the prosthetic restorations for posterior teeth. Fixed bridges were twice more frequently used for anterior teeth during this period than during the previous period. For a single missing tooth, fixed bridges amounted to 90% and removable partial dentures to 10%, as they did in the previous period. For two or more missing teeth, the use of fixed bridges was some what increased compared to the previous period. As in the previous report, the use of so-called one-tooth denture was most frequent for missing lower first molar. For bridges, the average number of missing teeth and that of abutment teeth were 1.3 and 2.4 respectively. Bridges for one missing tooth with two abutment teeth were most frequently used. For removable partial dentures, the average number of missing teeth and that of abutment teeth were 6.1 and 2.8 respectively. Removable partial dentures for two missing teeth with two abutment teeth were most frequently used. Full cast crowns were most frequently used for bridge retainers, followed by facing crowns. Facing crowns were used more than twice as frequently during this period as they were during the previous period.(ABSTRACT TRUNCATED AT 250 WORDS)
Denture, Complete↗