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Biomedical subjects

J Kaur

Publications and source records attributed to J Kaur.

At least 109 records · Page 6Linked to original sources
PubMed1988

Enterotoxigenicity, klebocinogeny and antibiotic resistance pattern of food isolates of Klebsiella pneumoniae.

The enterotoxigenicity, klebocinogeny and susceptibility to antibiotics for 100 strains of Klebsiella pneumoniae isolated from various food samples is reported. 49% of the samples supported growth of K. pneumoniae: a high proportion of these strains were isolated from sweets and snacks (51%). Enterotoxigenicity as determined by a coagglutination test with anti-LT4 coated staphylococci was observed in 61 of 100 Klebsiella isolates. 21% of the strains were found to be klebocin producers against the K. pneumoniae WC indicator strain. A significant correlation was observed between klebocinogeny and enterotoxigenicity (p less than 0.05). Most strains (91 and 74% respectively) were inhibited by gentamicin and nalidixic acid. Most of the strains were multiply drug-resistant.

Bacteriocins↗
PubMed1985

Anxiety and adjustment levels among the visually and hearing impaired and their relationship to locus of control, cognitive, social, and biographical variables.

Forty-five hearing-impaired and 40 visually impaired male residential school children aged 6 to 16 years were administered measures of adjustment, anxiety, and locus of control (LOC). Scores related to intelligence and academic level, teacher and peer acceptance, and biographical variables were also obtained. The findings show that the correlates of anxiety and adjustment differed qualitatively as well as quantitatively between the groups.

Achievement↗
PubMed1983

Protection with combinations of hydroxytryptophan and some thiol compounds against whole-body gamma irradiation.

Protection of mice (as measured by survival at 30 days) against whole-body gamma exposure by prior treatment with combinations of hydroxytryptophan (HT) and some thiol drugs, is reported here. The combination of HT and aminoethylisothiuronium bromide hydrobromide (AET) was much more effective than HT alone against the effects of 10.5 and 12.5 Gy of gamma ray exposure. A combination of HT and beta-mercaptopropionylglycine (MPG) gave marked protection against a 10.5 Gy exposure but not against 12.5 Gy. The combination of HT with cysteine or cysteamine also provided reasonable protection against 10.5 Gy. The combination of HT and cystamine rendered a low level of protection against 10.5 Gy, but again it was better than HT alone. The thiol drugs by themselves in the doses used in combination were ineffective in rendering protection after whole body gamma ray exposure.

Animals↗
PubMed1980

Membrane-bound protein kinase activity in acetylcholine receptor-enriched membranes.

Membrane protein phosphorylation may be a general regulatory mechanism mediating the response of cells to exogenous metabolic and physical signals. We have determined that the membrane-bound acetylcholine receptor is the major substrate phosphorylated in situ by a nearby membrane protein kinase. Moreover, these same membranes also contain phosphoprotein phosphatase activity which dephosphorylates the membrane-bound receptor. These findings suggest that reversible phosphorylation of the actylcholine receptor may be critical for receptor function at the synapse. Therefore, it is necessary to define the properties of the enzymes which mediate this phosphorylation-dephosphorylation mechanism. In this report we describe the properties of the first component of this system, the membrane-bound protein kinase in receptor-enriched membranes from the electric organ of Torpedo californica. Only ATP is effective as a phosphate donor for this cyclic AMP-independent membrane kinase; GTP does not support phosphorylation of the receptor. Both casein and histone can also be phosphorylated by the membrane protein kinase, but casein is a better substrate. Although phosphorylation of the receptor appears to be regulated by cholinergic ligands and K+, casein phosphorylation is not specifically affected by these agents. Moreover, while phosphorylation of the acetylcholine receptor is maximal in receptor=enriched membranes, casein phosphorylation is similar in all membrane fractions prepared from the electric organ. Taken together, these findings suggest that the membrane protein kinase activity in receptor-enriched membranes is similar to most other membrane kinases. Therefore, the unique characteristics of membrane-bound acetylcholine receptor phosphorylation appear to be determined by the receptor and its availability as a substrate for the membrane kinase.

Acetylcholine↗
PubMed1979

Purification and characterization of multiple species (isolectins) of a slime mold lectin implicated in intercellular adhesion.

An improved purification procedure for the carbohydrate-binding proteins (lectins) of cohesive Polysphondylium pallidum cells has been devised. The procedure uses extraction of cells with lactose-containing buffer followed by ammonium sulfate precipitation and affinity chromatography of the redissolved precipitate on a column of acid-treated Sepharose 6B. All hemagglutination activity is adsorbed to the column and recoveries are about 70% of the activity of the starting cell lysate. Sodium dodecyl sulfate-gel electrophoresis of the protein obtained with this procedure resolved three subunits with molecular weights of 26,500 (A), 26,000 (B), and 25,000 (C). Three species are resolved by isoelectric focusing with apparent pI values of 6.4 (I), 7.3 (II), and 7.5 (III) which contain Subunits A, B, and C in the following ratios: I, B:C at 2:1; II, A:B at 2:1, and III, A:B at 1:2. All three isoforms agglutinate rabbit and human type O erythrocytes and are thus isolectins. Isoforms II and III are separated from Isoform I by galactose-gradient elution of the Sepharose 6B column. Isoforms II and III aggregate extensively (nonamers and multiples thereof), but reduction with 2-mercaptoethanol reverses this process yielding a single species of Mr = 73,000 (trimer). Isoform I exists as trimers and hexamers and reduction has no effect on this distribution. Amino acid compositions and tryptic peptide maps of S-[14C]carboxymethyl-isolectins indicate that Subunits A and B are very similar and may represent the same peptide chain, while Subunit C is a peptide quite distinct from A and B.

Amino Acids↗
PubMed1978

1,2-Propanediol-induced changes in plasma and tissue lipids of rats.

Oral administration of 1,2-propanediol to rats in a daily dose of 1 ml of 28.4% aqueous solution per 100 g body weight for 30 days caused a significant decrease in the total lipids, fatty acids, phospholipids, and triglycerides of plasma, liver, and heart. The cholesterol content in plasma decreased while that in the tissues increased significantly. The accumulation of cholesterol in tissues tends to discourage long term use of 1,2-propanediol even by the oral route.

Animals↗
PubMed1977

Nonidentical subunits of human erythrocyte phosphofructokinase.

Human erythrocyte and muscle phosphofructokinase (PFK) were purified completely by improved procedures. SDS-acrylamide gel electrophoresis in a discontinuous buffer system revealed two subunits (R and M) of erythrocyte PFK, the slower one (M) corresponding to the single subunit of muscle PFK. The staining intensity ratio R:M of the two bands of erythrocyte PFK was 2:1 or less. This suggests that native erythrocyte PFK contains multiple isoenzymes with different proportions of R and M, some being lost during purification. Nevertheless, isoelectric focusing showed single peaks of erythrocyte PFK (pI 5.0) and muscle PFK (pI 6.6), perhaps because of aggregation of erythrocyte PFK isoenzymes. Erythrocyte PFK from a patient with muscle PFK deficiency had a pI of 4.6 and could not be precipitated by antiserum against muscle PFK, findings compatible with the putative structure R4.

Chromatography, DEAE-Cellulose↗
PubMed1975

Splenectomy for complications of chronic granulocytic leukaemia.

13 patients with chronic granulocytic leukaemia (C.G.L.) which was unsatisfactorily controlled underwent splenectomy. 3 out of 4 patients with hypersplenism did well, as did 5 out of 8 patients in whom the C.G.L. had undergone metamorphosis to a refractory phase. In a case of C.G.L. complicated by severe myelofibrosis the need for transfusion was reduced but survival was short. Splenectomy should be considered when C.G.L. in its chronic phase is complicated by hypersplenism, and may be considered as a part of the treatment after C.G.L. has undergone metamorphosis to a refractory phase. However, a favourable outcome is unlikely for patients over 65 years, and in the presence of coexistent illnesses, rapidly progressive metamorphosis to an acute phase, or severe bone-marrow failure from any cause. In C.G.L., elective splenectomy early in the chronic phase must be clearly distinguished from splenectomy performed at a later stage when the disease is not well controlled.

Adolescent↗