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Biomedical subjects

J Kaplan

Publications and source records attributed to J Kaplan.

At least 433 records · Page 24Linked to original sources
PubMed1976

Identification of T cell lymphoma tumor antigens on human T cell lines.

All human T lymphoblast cell lines have been derived from subjects with leukemia secondary to thymic lymphoblastic lymphoma, a T cell malignancy, suggesting that such lines represent established cultures of neoplastic T cells. Based on this observation, we prepared rabbit antisera to T cell line HSB-2, removed reactivity for histocompatibility antigens and normal T cells by absorption with autocthonous B cell line CCRF-SB and normal thymocytes, and tested the absorbed antisera by complement-dependent cytotoxicity against a panel of normal and malignant cells. A representative antiserum reacted with all 4 T cell lines (mean cytotoxic index =56) and with tumor cells from 4 patients with T cell lymphoma (mean cytotoxic index = 50) but did not react with tumor cells from 6 patients with other types of leukemias (mean cytotoxic index = 2), with 3 B cell lines (mean cytotoxic index = 1), normal peripheral blood lymphocytes (mean cytotoxic index = 5), or normal thymocytes (mean cytotoxic index = 6). We conclude that appropriately absorbed antisera to human T cell lines detect T cell lymphoma tumor antigens.

Antigens, Neoplasm↗
PubMed1976

The psychedelic model of schizophrenia: the case of N,N-dimethyltryptamine.

The authors review the research on N,N-dimethyltryptamine (DMT) as a possible "schizotoxin." DMT produces psychedelic effects when administered to normal subjects, the means are present to synthesize it in man, it has occasionally been found in man, and tolerance to its behavioral effects is incomplete. However, DMT concentrations have not been proven to differ significantly in schizophrenics and normal controls. Also, in vivo synthesis of DMT has not been convincingly demonstrated, and the psychological changes it produces do not closely mimic the symptoms of schizophrenia. The authors conclude that more data are necessary before the validity of this theory can be determined.

Chlorpromazine↗
PubMed1976

Quantitation of circulating T and B lymphocytes in children with whooping cough.

The numbers of circulating T and B lymphocytes in seven children with whooping cough due to Bordetella pertussis and eight control subjects were determined. All the children with whooping cough had an absolute lymphocytosis (mean 29,142/mm3vs. 5,225/mm3) and by surface marker criteria both T cells and B cells were increased (mean T cells, whooping cough 15,794/mm3 vs. 3,516/mm3 controls; mean B cells, 13,393/mm3 whooping cough vs. 1,706/mm3 controls). However, the ratios of T cells to B cells in the whooping cough (1.4) and control group (1.9) did not differ significantly. This proportional increase in both T and B lymphocytes indicates that whatever mechanism are responsible for lymphocytosis in B. pertussis infection affect both populations in a similar manner.

B-Lymphocytes↗
PubMed1976

Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts.

Absorption studies demonstrate that T-cell lymphoma-associated antigens detected by rabbit antisera to human T-lymphoblast cell lines are present in suspensions of cord blood lymphocytes and phytohemagglutinin-stimulated adult blood lymphocytes in amounts similar to those found in T-cell lymphoma tumor cell suspensions. Smaller amounts of antigen activity are found in suspensions of tonsil cells, thymocytes, and unstimulated adult blood lymphocytes. Little or no antigen activity is found in suspensions of lymphoblasts from patients with other types of leukemia or from B-cell lines. T-cell depletion removes antigen activity from suspensions of normal lymphocytes. These findings suggest that T-cell lymphoma-associated antigens may be fetal antigens expressed by activated T-cells.

Adult↗
PubMed1975

Studies on the turnover of plasma membranes in cultured mammalina cells. I. Rates of synthesis and degradation of plasma membrane proteins and carbohydrates.

The rate of turnover of membrane proteins and membrane-bound carbohydrates in exponentially growing and in confluent contact-inhibited cultures of strain MK-2 cells was investigated. Cells were labelled with [14-C]leucine and [3-H]glucosamine, incubated in isotope-free medium and, at various times thereafter, the cells were harvested and membranes isolated from them. The rate of decay of the protein and carbohydrate components was determined from specific activity dilution of the labeled components in the isolated membranes. Although the rate of membrane synthesis is different in exponential and contact-inhibited cells, the rate of degradation (turnover) of membrane proteins and carbohydrates was found to be the same (25% per generation (42 h) or 0.6%/h).

Animals↗
PubMed1975

Studies on the turnover of plasma membranes in cultured mammalian cells. II. Demonstration of heterogeneous rates of turnover for plasma membrane proteins and glycoproteins.

The relative rate of turnover of individual membrane proteins and glycoproteins in exponentially growing and contact-inhibited MK2 cells was investigated. Plasma membranes were isolated from cells that had been sequentially labelled with 14-C and 3-H isotopes of leucine and glucosamine. The membranes were then solubilized in sodium dodecylsulfate and their polypeptides separated by acrylamide gel electrophoresis. The 3-H/14-C ratios of the individual polypeptides reflected their relative rates of turnover. The proteins and glycoproteins of the exponentially growing cells exhibited markedly heterogeneous rates of turnover. In contrast, polypeptides in membranes of contact-inhibited cells exhibited a lesser degree of heterogeneity of turnover. In both exponential and contacted cell membranes a glycoprotein with a high apparent molecular weight exhibited the fastest rate of turnover.

Animals↗
PubMed1975

Psychiatric evaluation services to court referred drug users.

This paper reports on the description and outcome of one hundred youthful drug abusers referred from family court for psychiatric evaluation and treatment recommendation. The average referral, both male and female, was a 16-year-old white Catholic from a middle class background who was diagnosed as having a personality disorder. Significantly more males than females showed pathology in early childhood such as behavior problems in school and hyperactivity. Follow-up data collected up to 6 months after evaluation and treatment recommendation indicated that approximately half of the clients showed improvement in terms of work and/or school adjustment, social relations, and drug use.

Achievement↗