Droloxifene--a novel antiestrogen in endocrine dependent human tumors xenotransplanted into nude mice.
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Animals↗
Biomedical subjects
J Kömpf
Publications and source records attributed to J Kömpf.
Human factor H (beta 1H-globulin): linkage analysis.
Linkage data on human factor H (HF) and 22 other human genetic markers are presented. Close linkage at theta less than 0.10 can be ruled out for a series of marker systems (Rh, PGM1, ACP1, Jk, Tf, Gc, MNSs, ME2, HLA, GLO1, ORM, Gt, PI, Hp, GPT). Strong evidence for linkage was obtained for peptidase A (PEPA) with lods greater than 3.0 at theta = 0.10 in males and at theta = 0.20 for the sexes combined. From this result the HF locus can be provisionally assigned to chromosome 18.
Complement C3b Inactivator Proteins↗
Formal genetics of esterase D (EC 3.1.1.1): evidence for a sex-phenotype association.
The formal genetics of esterase D (EC 3.1.1.1) was studied in family data and mother/child pairs. A general agreement with mendelian expectations was found. However, a significant sex-phenotype association was detected in families from northwestern Portugal as well as in mother/child pairs and family data from southwestern Germany.
Carboxylesterase↗
Distinct types of donor and recipient T cell clones functionally related to host-v-graft reactivity and autologous remission after bone marrow transplantation for severe aplastic anemia.
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Adult↗
Refinement of HLA gene mapping with induced B-cell line mutants.
The lymphoma cell line BJAB.B95.8.6 was gamma-irradiated to induce mutations of major histocompatibility complex (MHC) encoded genes. Cloned "wild-type" cells were phenotyped HLA-A1, A2, B13, B35, Bw4, Bw6, Cw4, DR5, DRw52, DQw1, DQw3, DPw2, DPw4, GLO1 1, PGM3 2-1, and ME1 0 and possessed two apparently normal chromosome 6s prior to mutagenesis. Loss mutants were selected 5 days after 3 Gy gamma-irradiation employing three complement-fixing monoclonal antibodies specific for HLA-A2 (TU101) and Bw4 (TU48, TU109). Fifteen independently arising mutants were isolated and cloned. Typing with monospecific alloantisera and cell-mediated lympholysis revealed the presence of HLA-A1, B35, Bw6, Cw4, DR5, DRw52, DQw3, and DPw4 specificities on all mutant clones. HLA-A2, B13, and Bw4 were absent. Mutants differed in their expression of class II antigens. One group retained DQw1 and DPw2, another was DQw1-, DPw2+, and a third was DQw1-, DPw2-. Karyotyping of the "wild-type" line and selected mutant clones showed that the loss of HLA specificities correlated with deletions which map the HLA-A and -B loci directly to the distal part of the 6p21.33 region and the class II genes to the region 6p21.33 (proximal) to 6p21.31 (distal) on the short arm of chromosome 6.
Antibodies, Monoclonal↗
Linkage between the loci for mitochondrial malic enzyme (ME2) and coagulation factor XIIIA subunit (F13A).
The locus for the mitochondrial malic enzyme (ME2) seems to be closely linked to the gene coding for the A subunit of coagulation factor XIII (F13A). From 18 informative families with 54 children a maximum lod score of 4.33 was obtained at a recombination fraction of 0.10. Since the assignment of F13A to the short arm of chromosome 6 was recently confirmed, the locus for ME2 can be added to this linkage group. The map order seems to be: GLO1 - HLA - F13A - ME2.
Adult↗
DNA polymorphism of the major histocompatibility class I genes and their association with serologically defined haplotypes.
DNA of unrelated persons as well as members of families that were totally or partially homozygous or completely heterozygous on the loci of the major histocompatibility class I genes has been isolated from peripheral blood lymphocytes and blot hybridized with the class I pseudogene pHLA 12.4 probe. The autoradiographic DNA patterns were discussed and compared with well-defined serological features. Positive associations with serologically typed alleles had been demonstrated for HLA-A1,11; -A2; -A3; -B7; -B14; -B35; -Bw41; and -Cw5.
Alleles↗
Monosomy 6 in a human lymphoma line induced by selection with a monoclonal antibody.
The human Epstein Barr Virus-superinfected B lymphoma cell line BJAB-B95.8.6 was mutagenized by gamma irradiation, and HLA mutants were selected with the HLA-Bw6-specific monoclonal antibody SFR8-B6. One of the mutants obtained, BM19, had lost one of the chromosomes 6 present in the wild type cells. Electrophoretic analysis of phosphoglucomutase isozyme PGM3 and erythrocyte glyoxalase 1 from both cells supports this conclusion. The HLA antigens expressed on BM19 were HLA-A2, B13, Bw4, C-, DR2 (questionable), DRw52 (weak) and DQw1. This constitutes one of the haplotypes of the wild type cells, the other (lost from BM19 cells) being HLA-A1, B35, Bw6, Cw4, DR5, DRw52 (strong) and DQw3. Possibilities to employ BM19 cells for the analysis of the major histocompatibility complex and other chromosome 6-encoded genes as well as their products are discussed.
Antibodies, Monoclonal↗
Cell fusion responsible for horizontal oncogenesis by human tumors in nude mice.
Human tumor xenotransplants derived from 4 different carcinomas were established in athymic mice and maintained by serial passage. In vivo human/mouse cell fusion and induction of neoplastic growth of adjacent mouse tissue was investigated in serial passages by isoenzyme analysis, by chromosome analysis and by cloning of induced tumorigenic mouse cells. In 2 xenotransplants human/murine hybrid isoenzyme complexes of pyruvate-glutamate transaminase, glucosephosphate isomerase and mitochondrial malate dehydrogenase were detected. Metaphases containing human and murine chromosomes were found in the first in vitro passage of excised xenotransplants. Cloning of induced mouse tumor cells yielded tumor cell lines tumorigenic in athymic as well as in immunocompetent mice.
Animals↗
Association between rapidly progressive glomerulonephritis and the properdin factor BfF and different HLA-D region products.
Frequencies of the HLA antigens ABC, DR and MT, as well as of the properdin factor alleles were determined in 24 unrelated patients presenting with immune complex mediated idiopathic rapidly progressive glomerulonephritis (RPGN) type II. As in Goodpasture syndrome (RPGN type I with pulmonary hemorrhage), a significant association with the B-cell alloantigen HLA-DR 2 was demonstrated (relative risk for HLA-DR 2 positive individuals was 3.54; P less than 0.01). In addition a marked increase of the HLA specificity MT 3 was shown, which is supposed to belong to an antigen system of a second HLA-D region locus. The highest relative risk of 14.67 (P less than 0.00001), however, was calculated for all patients carrying the BfF phenotype. Increased numbers of patients positive for HLA-DR 2 and -MT 3, as well as BfF suggested immune response genes or disease-related mutations on different haplotypes responsible for a MHC (major histocompatibility complex) associated predisposition of RPGN type II.
Adolescent↗
[Enzyme activity of isolated leukocyte populations. I. Cytochemical and zymographic studies of stored blood under various storage conditions].
Heparinized venous blood was stored under sterile conditions at different temperatures (4 C, 20 C, 37 C) for various intervals (0-7 days). After storage the granulocytes and lymphocytes were isolated with routine methods. Naphthol AS-D-chloroacetate esterase as a granulocyte marker and acid alpha-naphthyl acetate esterase as a T-lymphocyte marker were identified on smears of the washed cell suspension. Different enzymes were identified in the cell sediment with electrophoresis. Relatively pure lymphocyte suspensions were obtained within the first 24 h. After this time, however, the percentage of these mononuclear cells declined markedly. The percentage of isolated granulocytes varied slightly; there was a marked predominance of granulocytes (more than 70%) at all intervals investigated during the isolation. Cytochemical analysis of the granulocytes and lymphocytes indicated that the decrease in the percentage of enzyme-positive cells depends in each case on the duration of the storage interval. During the first 24 h, only PGM1 and GOTM could be identified in the lymphocyte suspension with horizontal starch gel electrophoresis. The enzymes PGM1, PGM3, PGI, MDH, GOTM, 6-PGD, ADA could always be identified in the granulocyte suspension; AK, FUCA, MEM could be occasionally identified; and GPT and GLO could never be identified.
Blood Preservation↗
[Enzyme activity of isolated leukocyte populations. II. Cytochemical and zymographic studies of cadaver blood].
Blood was taken from the femoral vein of 17 autopsied cadavers with different diagnoses and postmortal intervals (10-120 h). The granulocytes and lymphocytes were isolated with routine methods. The cell suspensions were subject to morphologic, enzyme-cytochemical (naphthol AS-D chloroacetate esterase) and electrophoretic (PGM1, PGM3, GOTM, PEPA, MEM, FUCA) investigations. The following results were obtained: Erythrocyte-free cell suspensions are only found during short postmortal intervals; Isolation of pure lymphocytes from cadaver blood using the described method is only possible very early in the postmortal interval (within 10 h); The isolated granulocytes contain an impressively high percentage of eosinophilic granulocytes; The percentage of naphthol AS-D-chloroacetate esterase-positive granulocytes is considerably lower in stored, conserved blood than in blood smears; Identification of the above mentioned enzymes from isolated granulocytes from all cadavers is possible by electrophoresis.
Enzymes↗
Enzyme activity in human mononuclear blood cells.
Two different kinds of mononuclear blood cell samples (mixed blood cell population consisting of lymphocytes, monocytes, and platelets; pure lymphocyte population) were tested for enzyme activity of mitochondrial malic enzyme, peptidase A, and alpha-fucosidase. None of the three enzymes was demonstrable in the pure lymphocyte preparation; the mixed cell population, however, exhibited a distinct enzyme activity. Positive reactivity is thus obviously dependent upon monocytes and/or platelets.
Blood Platelets↗
Aminolevulinate dehydratase (E.C. 4.2.1.24): linkage analysis.
Linkage data on aminolevulinate dehydratase (ALADH, E.C. 4.2.1.24) and a series of other human genetic markers are presented. One hundred and two families (25 of them being informative) from southwestern Germany were tested. Close linkage (theta = 0.05) between ALADH and the following markers could be excluded: Rh, PGM1, Fy, ACP1, MNSs, HLA, Bf, GLO, PGM3, Jk, Pi, PGP, K, GPT. There is some evidence of possible linkage with HPA.
Electrophoresis, Starch Gel↗
Linkage of Pgm-3 in the house mouse and homologies of three phosphoglucomutase loci in mouse and man.
The discovery of a third phosphoglucomutase locus (Pgm-3) in the house mouse is reported. Three alleles are recognized on the basis of differences in electrophoretic mobility and enzymatic activity. Pgm-3A (fast mobility and high activity) is present in inbred strain C57BL/10J and 24 other strains; Pgm-3b (slow mobility and high activity) is present in LP/Pas and six other strains; and Pgm-3c (no detectable activity in any tissue tested) is present in strain DBA/2J and 14 other strains. Seventy-four recombinant inbred strains derived from progenitors that differed at Pgm-3 were used to study genic linkage. Pgm-3 is on chromosome 9 and is linked to Sep-1, d, Mod-1, and Ltw-3. Gene order and recombination frequencies are estimated as d 3.8 +/- 1.8%. Pgm-3 2.3 +/- 1.2% Mod-1. Substrate specificities and cofactor requirements show that mouse Pgm-1 is homologous with human Pgm-2, mouse Pgm-2 with human Pgm-1, and mouse Pgm-3 with human Pgm-3.
Alleles↗
Strong association of idiopathic membranous nephropathy (IMN) with HLA-DR 3 and MT-2 without involvement of HLA-B 18 and no association to BfF1.
The present study investigated the distribution of HLA-antigens and Bf alleles in a group of 21 patients suffering from biopsy proven Idiopathic Membranous Nephropathy (IMN). A statistically significant increase of HLA-DR3 (76.2%) was detected in the patient group as well as an increase of MT-2 (86%), which is a new supertypic specificity defined within the 8th International Histocompatibility Workshop 1980. No association between BfF1 and IMN can be deduced from this study. Only the common Bf alleles (BfFF, BfFS, BfSS) were found in the patient group presented here. Also the previously reported association between HLA-B18 and IMN was not corroborated by this study. Not a single IMN-patient typed positive for HLA-B18. Thus the presence of two different HLA-DR-Bf-B linkage groups (HLA-DR3/BfF1/HLA-B18 and HLA-DR3/Bf; all alleles/HLA-B8) may point to at least two different immunological mechanisms underlying IMN.
Adolescent↗
Galactose-1-phosphate-uridyltransferase (E.C. 2.7.7.11): a simple routine method for detecting individuals heterozygous for the silent allele Gt 0.
A simple routine method for detecting individuals who are heterozygous for the silent gene Gt 0 is presented. This method consists of a combination of electrophoresis and densitometry. The results confirm the theoretical expectation that these individuals would exhibit about 50% of the enzyme activity found in the corresponding normal phenotype.
Alleles↗
Formal genetics of phosphoglycolate phosphatase (PGP): investigation on 272 mother-child pairs.
PGP (phosphoglycolate phosphatase, E.C. 3.1.3.18) phenotypes were determined by starch gel electrophoresis in 272 mother-child pairs from S. W. Germany. The results confirm and formal hypothesis of three alleles, PGP1, PGP2 and PGP3 at an autosomal locus PGP.
Adult↗