Relation of neuroendocrine function to reproductive decline during aging in the female rat.
Explore the source record for details and available documents.
Aging↗
Biomedical subjects
J K Lu
Publications and source records attributed to J K Lu.
Radiation exposure of anesthesiologists.
STUDY OBJECTIVE: To determine the level of radiation exposure of anesthesiologists in the operating rooms and cardiac catheterization laboratory. DESIGN: Prospective study of all anesthesia fellows. SETTING: Operating rooms (ORs) and radiology department of a tertiary care pediatric hospital. SUBJECTS: Anesthesiologists caring for patients in a pediatric hospital requiring anesthesia or monitored anesthesia care. (Patients were not directly studied.) MEASUREMENTS AND MAIN RESULTS: Anesthesiologists wore standard radiation safety film badges, which were sensitive to cumulative doses of radiation greater than 10 mrem. In the ORs, anesthesiologists were exposed to less than 10 mrem/mon. In the cardiac catheterization laboratory, they had dosimetric readings that ranged from 20 to 180 mrem/mon and frequently exceeded the guidelines for nonradiation workers. CONCLUSIONS: There is no need for routine dosimetric monitoring of anesthesiologists working in the OR setting, since this is associated with negligible radiation exposure. Monitoring of radiation film badges should be considered for anesthesiologists who frequently work in fluoroscopy areas, such as the cardiac catheterization laboratory. All personnel working near radiation sources should wear appropriate shielding and, whenever patient safety permits, distance themselves as far as possible from the source of radiation.
Anesthesiology↗
Integration, expression and germ-line transmission of foreign growth hormone genes in medaka (Oryzias latipes).
Gene constructs consisting of human growth hormone (hGH) gene driven by promoter/regulatory sequence of mouse metallothionein (mMT), viral thymidine kinase (vTK), rat cholecystokinin (rCCK), or chicken beta-actin (cBA) gene were injected into the cytoplasm of fertilized medaka eggs via the micropyle. More than 49% of the injected embryos survived at hatching. Up to 26% of the survivors showed integration of the introduced gene construct, as determined by polymerase chain reaction analysis and subsequent confirmation by Southern blot hybridization of the genomic DNA. A significant fraction of F1 progeny, derived from crosses between transgenic founders and the nontransgenic individuals, inherited the transgene. Expression of hGH gene was also observed in some of the P1 founders and F1 transgenic progeny carrying mMT-hCG or cBA-hGH gene. Furthermore, the growth performance of the P1 mMT-hGH and cBA-hGH transgenic founders and F1 cBA-hGH F1 transgenic progeny was significantly greater than their full sibling, nontransgenic individuals. In addition to the microinjection experiment, a gene construct containing the long-terminal repeat (LTR) sequence of avian Rous sarcoma virus (RSV) and rainbow trout (rt) GH2 cDNA was introduced into embryos of medaka by electroporation using an exponential decay electroporator. Approximately 70% of the electroporated embryos survived at hatching, and 20% of the survived individuals integrated RSVLTR-rtGH2 cDNA into their genomes. These two techniques will greatly enhance the ability to study regulation of gene expression in transgenic animals during differentiation and development.
Animals↗