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Biomedical subjects

J K Frost

Publications and source records attributed to J K Frost.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

Epithelial atypias associated with intrauterine contraceptive devices (IUD).

A total of 99 gynecologic smears with atypical cells from 74 patients wearing IUDs was reviewed. Endometrial and cervical tissues were examined. The cases were categorized into two groups, reparative and epithelial atypias. The latter category was further divided into three subgroups: squamous, columnar, and indeterminate type. The squamous atypia has classical features and is believed to be not significantly related to the IUD. Columnar atypia can strongly suggest cells shed from an adenocarcinoma. Indeterminate type of atypia is characterized by abnormal cells which can resemble cells shed from carcinoma in situ. Unlike the latter, the atypical cells from IUD are usually multinucleated, contain nucleoli, and are not associated with cells from severe cervical atypia. They are probably of endometrial origin.

Adolescent↗
PubMedDate not supplied

Occurrence and identification of intracellular calcium crystals in pulmonary specimens.

Scanning electron microscopy, x-ray analysis studies and histochemical techniques have identified unusual birefringent intracellular calcium carbonate crystals seen in the sputum specimens of a 68-year-old man with cavitary pulmonary lesions. He was clinically diagnosed and treated for pulmonary tuberculosis. Although other etiologies are possible, we contend that the calcium salt crystals identified in the sputum in this patient represent a peripheral manifestation of tuberculosis which is rarely noticed in the sputum specimens.

Aged↗
PubMedDate not supplied

A comparative cytopathologic study of noninvasive and invasive squamous cell carcinoma of the lung.

Atypical cells and tissue fragments from the sputum of patients with early and advanced stages of squamous cell carcinoma of the bronchus are objectively characterized and quantitatively compared in this paper. Four classes of single-cell features of cytoplasm, nucleus, nucleolus and nuclear-cytoplasmic ratio are analyzed as a function of cell size and tumor development stage. Distinct differences in the cellular patterns are observed which may enhance cytologic discrimination between noninvasive and invasive stages of squamous cell carcinoma of the bronchus. Initial results justify the application of more sensitive measurement techniques (i.e., automated cytology) to an enlarged data base.

Carcinoma in Situ↗
PubMedDate not supplied

Identification of viruses in the urine of renal transplant recipients by cytomorphology.

This study was designed to reassess the cytomorphology of viral infections in urinary cells obtained from renal transplant patients and to examine the association, if any, between these cytologic changes and the transplant rejection. A total of 2,354 cytologic specimens obtained from 91 renal transplant recipients was evaluated. A combination of techniques, including cellulosic filters, immunofluorescence, hemagglutination inhibition and electron microscopy, was utilized. Cytologic observations were correlated with the patient's clinical history. Thirty-eight patients revealed cytologic evidence of viral infections (herpes simplex, cytomegalovirus and papovavirus). These viral infections had distinct cytomorphology. Cytomegalovirus infection may manifest as intracytoplasmic, orangeophilic inclusions, in addition to the classical intranuclear inclusion. In the majority of renal transplant patients there appeared to be no relationship between the viral infection and the renal transplant rejection episodes.

Adolescent↗
PubMedDate not supplied

A simple lavage method for the cytologic sampling of DMBA-induced carcinomas of the hamster cheek pouch.

An effective, simple sampling device was developed to obtain improved cytologic specimens from the cheek pouches of hamsters; it was tested in the sampling of squamous-cell carcinomas induced with 9,10-dimethyl-1,2-benzanthracene (DMBA). Cellular samples collected by this modified syringe lavage-aspirator using Hanks' balanced salt solution contained an average of 1.3 X 10(5) cells per milliliter. Of the heterogeneous contents of the cheek pouch (food, debris, erythrocytes, leukocytes, macrophages, squamous cells, atypical cells and cancer cells), the cells of diagnostic significance from cancer and dysplasia were increased by this procedure to 3.7% of the cells recovered, as compared to 2.5% of the cells obtained by direct scraping of the same pouches. The inexpensive, disposable device allowed repeated sampling of cheek pouches without causing injury to the animals.

9,10-Dimethyl-1,2-benzanthracene↗
PubMedDate not supplied

Amebae resembling Entamoeba gingivalis in the genital tracts of IUD users.

A total of 2,252 vaginopancervical (Fast) smears were studied as a continuation of an ongoing program to further investigate the occurrence of amebae in the genital tracts of women using intrauterine devices (IUDs). Of the 947 IUD users, 1% harbored trophic amebae. No amebae, however, were found in any of the 1,164 smears from non-IUD wearers. In all instances the amebae were associated with Actinomyces. Both organisms disappeared promptly after removal of the IUD. In preparations stained according to the Papanicolaou, iron-hematoxylin and Gomori-Wheatley trichrome methods, the amebae could be diagnosed as belonging to the genus Entamoeba. These trophozoites failed to stain with fluorescein-labeled anti-Entamoeba-histolytica serum. Although on the basis of the presently available evidence these trophozoites cannot be assigned with certainty to any known species, they appear to share many morphologic characteristics with Entamoeba gingivalis.

Actinomyces↗
PubMedDate not supplied

Urinary cytologic changes after radiotherapy of renal transplants.

Significant changes in the urinary cellular pattern can be attributed to renal allograft radiation, as distinct from those associated with immunologic rejection of the transplated kidney. These changes consist of nuclear multiplicity, wrinkling of the nuclear membrane, a "coffee bean" appearance of the nucleus, and increase in nuclear size, areas of decreased density and vacuolation of the cytoplasm, and perinuclear halos. The severity of the changes was categorized as "mild", "moderate", or "marked", according to the number of the above changes which were present, and was more evident in cells of transitional epithelial origin than of tubular origin. Some correlation was found between the severity of the cellular radiation changes and the survival time of the kidney.

Female↗