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Biomedical subjects

J Jouquan

Publications and source records attributed to J Jouquan.

At least 37 records · Page 2Linked to original sources
PubMed1990

Comparison of labial and sublingual salivary gland biopsies in the diagnosis of Sjögren's syndrome.

This study was designed to compare labial and sublingual salivary gland biopsies in the diagnosis of Sjögren's syndrome (SS). Fourteen labial and 29 sublingual specimens were considered positive. There was a better correlation between infiltration of the ductal structure and the focus score in the sublingual salivary gland biopsy than in the labial salivary gland biopsy. Use of sublingual salivary gland biopsy as an additional diagnostic tool in SS is therefore suggested.

Adult↗
PubMed1990

Serological arguments for classifying Raynaud's phenomenon as idiopathic.

Twenty-five patients with idiopathic Raynaud's phenomenon were followed prospectively for a mean period of 48 months. Clinical and laboratory assessments were performed on admission and on followup. The sera were analyzed for the presence of autoantibodies (antinuclear, antiskeleton and antiorganelle antibodies). Sixteen patients were antinuclear antibody positive and 2 anticentromere antibody positive. Eight patients produced antivimentin, 5 antimitochondrial, 4 anti-Golgi complex, and 3 anticentriol antibodies. Eleven patients produced antidesmosome antibodies. Only one patient (anti-RNP and antidesmosome antibody positive) developed a systemic disease (mixed connective tissue disease) during followup. The initial screening of sera may help to classify Raynaud's phenomenon as idiopathic more accurately.

Adolescent↗
PubMed1989

[Short loss of consciousness: etiology and diagnostic approach. Results of a prospective study].

Transient loss of consciousness is a frequent reason for hospitalization, but very few prospective studies have been devoted to this topic. Our study involved 150 patients who were admitted for sudden and total loss of consciousness (syncope) with spontaneous return to normality. All patients underwent thorough physical examination, standard laboratory tests, electrocardiography (ECG) and radiography of the chest. Depending on the results of this first evaluation, the patients were investigated for postural hypotension and had carotid sinus massage, electroencephalography (EEG), computerized tomography of the brain, cardiac Holter recording, electrophysiological exploration and, if necessary, other special examinations. The cause of the syncope was found in 93 cases (62 per cent); it was cardiac in 39 cases (bradycardia 25, tachycardia 14), vascular in 20 cases (vagal 14, postural hypotension 6), epileptic "grand mal" type in 32 cases and "miscellaneous" in 2 cases. The syncope occurred in a state of acute drunkenness in 14 cases and was unquestionably due to the absorption of medicines in 6 cases. Clinical findings and ECG or EEG provided the aetiological diagnosis in 82.7 per cent of the patients and the other, sophisticated examinations in 17.3 per cent. These results are similar to those of other prospective studies found in the literature. It may be concluded that the causes of the syncope are only found in about 2 out of 3 cases, that clinical data are all-important in the diagnostic approach and that complementary examinations are either unnecessary or yield little that is not already suggested by clinical examination.

Adolescent↗
PubMed1989

Serological abnormalities, including common idiotype PR4, in families with rheumatoid arthritis.

A broad range of autoreactivity among a group of 12 French patients with rheumatoid arthritis (RA) and 58 of their healthy first degree relatives has been identified. Over 15% of the patients were found to have antibodies to ssDNA, histone H1, H2A, and H2bB. Among the relatives, IgG and IgM rheumatoid factor and antibodies to ssDNA, H2A, and H4 were present in more than 10%. Even more remarkable, a common anti-DNA antibody idiotype designated PR4, known to be present in 70% of patients with systemic lupus erythematosus (SLE), was found in approximately 30% of both patients with RA and their healthy relatives. This is quite different from its lack of increased expression in relatives of patients with SLE and suggests that in the family members of those with RA a particular combination of environmental influence on germline gene expression is responsible.

Antibodies, Anti-Idiotypic↗
PubMed1988

Activation of peripheral blood lymphocytes in patients with primary Sjögren's syndrome.

The peripheral blood from 15 patients with primary Sjögren's syndrome and from 15 matched controls was examined for the presence of activated T and B lymphocytes, by using monoclonal antibodies directed to interleukin-2 (IL-2) and transferrin receptors, and to HLA-DR determinants. The number of circulating positive-T cells was significantly greater in the patients than in the controls, irrespective of disease activity. There were more of the CD8 cells than of the CD4 cells that expressed IL-2 receptors. There was a small but significant increase in activated B cells in the patients, since this population is virtually absent from the normal blood.

Adult↗
PubMed1988

CD5-expressing B lymphocytes in the blood and salivary glands of patients with primary Sjögren's syndrome.

CD5, the human counterpart of Ly-1 molecules in the mouse, are detectable but weakly expressed on a minute fraction of circulating B cells. The number of CD5 + B cells in the blood of patients with Sjögren's syndrome was slightly higher than in control blood, but it became statistically significant after treatment of the cells with phorbol myristic acetate. These numbers were even higher in patients with homogeneous serum bands than in the others. A few scattered cells were stained with anti-human IgM antibody on salivary gland sections, and among them 5-10% were found to be positive for anti-CD5.

Adult↗
PubMed1988

Chronic lymphocytic leukemic (CLL) cells secrete multispecific autoantibodies.

A subset of B cells expressing the CD5 marker, a 67 KD molecule, has been implicated in the pathogenesis of autoimmune disease. To study the immunoglobulin repertoire of CD5+ B cells we investigated chronic lymphocytic leukemic (CLL) cells, since the majority of the malignant clones express CD5. CLL were induced to secrete their IgM in vitro by phorbol 12-myristate 13-acetate (PMA) and the supernatants screened for binding to a panel of autoantigens. Twelve out of 14 CLL clones were autoreactive, binding to Fc of IgG, ssDNA, dsDNA, histones, cardiolipin, or cytoskeletal components. Many also bound to more than one antigen tested for, showing multispecificity. Our data suggest that a high proportion of CD5+ B cells are programmed to secrete multispecific autoantibodies.

Antibodies, Neoplasm↗
PubMed1988

Relation of antivimentin antibodies to anticardiolipin antibodies in systemic lupus erythematosus.

Tests for antivimentin antibodies (AVA) were performed on 50 systemic lupus erythematosus (SLE) and 63 control sera by indirect immunofluorescence and enzyme linked immunosorbent assay (ELISA). The prevalence was significantly raised in SLE (38% and 50% of sera positive for IgM-AVA and IgG-AVA, respectively, by immunofluorescence; 36% and 64% of sera positive for IgM-AVA and IgG-AVA, respectively, by ELISA) in comparison with the control sera. A significant correlation existed between IgM-AVA, on the one hand, and anticardiolipin antibodies (ACA) and anti-single-stranded DNA (ssDNA), on the other. A stepwise principal component analysis demonstrated that IgM-AVA and IgG-AVA accounted for 71% of the total variance in SLE (50 patients x 5 parameters = total variance). Twenty ACA positive serum samples from patients with syphilis were therefore tested for the presence of AVA, but hardly any were found to be positive. IgM-AVA from patients with SLE were inhibited by cardiolipin and absorbed with ssDNA. An association between AVA positivity and arthralgia was also shown in SLE.

Adolescent↗
PubMed1988

[The value of resorting to 2 technics for detecting anti-mitochondrial antibodies].

The presence of antimitochondrial antibody was determined by an indirect immunofluorescence assay (IIFA) and a quantitative complement fixation test (CFT) in 2,302 sera. This survey enabled us to calculate the sensitivity, specificity, positive and negative predictive values of the two techniques. It seemed valuable to use both techniques as the IIFA was more sensitive than the CFT whilst the CFT was more specific than the IIFA.

Antibodies↗
PubMed1988

Antibodies to histones in rheumatoid arthritis.

Using a newly developed enzyme-linked immunosorbent assay, antibodies to histones (H1, H2A, H2B, H3 and H4) were found in 32 out of 40 rheumatoid arthritis patients with antinuclear antibodies at a titer greater than or equal to 100 as measured by indirect immunofluorescence. The anti-H2A reactivity was higher in patients with secondary Sjögren's syndrome than in those without, but the highest antihistone reactivity and the most heterogeneous patterns were observed in patients who were receiving D-penicillamine.

Adult↗