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Biomedical subjects

J Joseph

Publications and source records attributed to J Joseph.

At least 199 records · Page 11Linked to original sources
PubMed1985

Expression of organ-specific antigens on capillary endothelial cells.

Our central thesis is that the endothelial cells which line capillaries of various organs are not all alike. Using monoclonal and conventional antibodies we demonstrate that capillary endothelial cells express on their cell surface an array of antigens that manifest organ selectivity. Brain-derived endothelial cells possess brain-associated antigens, ovary-derived endothelial cells share antigenic markers with other ovarian cells, and lung-derived endothelium possesses antigens that are primarily expressed on cells of the lung. Our experiments lead us to suggest that organ-associated determinants on the endothelial cell surface may play a role in the selective adhesion of tumor cells during metastasis, in site-limited vascular pathology, and in the regionally limited release of angiogenesis-induced factors.

Animals↗
PubMed1985

Orthostatic effect of imipramine and doxepin in depressed geriatric outpatients.

Blood pressure measurements were collected from 36 depressed geriatric outpatients (ages 55 to 81 years) enrolled in a double-blind, placebo-controlled study of the efficacy of doxepin and imipramine. Mean systolic postural changes were 25.9 mm Hg for imipramine, significantly higher than the 10.5 mm Hg for doxepin, and 12.4 mm Hg for placebo. The orthostatic drop in the imipramine group was only weakly related to dose and did not correlate with amount of pretreatment orthostatic hypotension or with duration of treatment. The increased orthostatic hypotension occurred early in treatment and at low doses of imipramine. Accordingly, caution is advised in the use of imipramine for the elderly.

Age Factors↗
PubMed1984

Separation of aerobic glycolysis from oxidative metabolism and contractility in rat anococcygeus muscle.

The relationship of aerobic glycolysis (JLac) and O2 utilization (JO2) to contractility and the activity of the Na+-K+ pump was investigated in rat anococcygeus smooth muscle. Removal of extracellular potassium concentration ([K+]o) caused an initial increase followed by a decrease in JLac below the resting rate. Restoration of [K+]o caused JLac to increase, and this response, but not the initial one observed in zero [K+]o, was prevented by ouabain. These changes in JLac were not related to contractility, since a similar response was observed in both phentolamine-treated and chemically denervated muscles that exhibited minimal force and no significant changes in JO2. Tension development of innervated muscles incubated in K+-free medium was related to changes in JO2. A logarithmic relationship between JLac and [K+]o suggested that changes in JLac resulted from effects on glucose transport or utilization secondary to changes in the membrane potential. In stimulated muscles JLac and JO2 were both positively correlated with isometric force and were not affected by ouabain. Separation of JLac from JO2 and force production under some conditions supports the hypothesis that a functional compartmentalization of metabolic pathways exists in anococcygeus muscle.

Aerobiosis↗
PubMed1984

Haptoglobin types and unipolar depression.

Haptoglobin (Hp) has been used in association studies with unipolar depression and has led to investigations on the possible association of Hp genotypes with disease. Because of the reported association between the Hp-2 allele and depression we phenotyped 65 elderly patients with unipolar depression and 40 elderly individuals without mental disorders. We failed to find an association between either of the Hp alleles and unipolar depression. The Hp2 gene frequencies for the depressed patients and the comparison group were 0.585 and 0.550, respectively, and these values were not significantly different. Both values were within the gene frequency range reported for Caucasian populations.

Aged↗
PubMed1984

Estimating the recombination frequency for the MN and the Ss loci.

Linkage analysis of 146 informative families for MN and Ss resulted in an estimate of the recombination frequency greater than previously reported. Our total is 7 recombinant children out of 467 individuals, including 1 confirmed recombinant (retested and HLA-compatible) and 6 not verified. The 95% confidence interval of our estimate of recombination is 0.0033-0.0167. Our results are compared with two earlier studies.

Female↗
PubMed1982

Monoclonal antibody against angiotensin-converting enzyme: its use as a marker for murine, bovine, and human endothelial cells.

A monoclonal antibody has been prepared against rat angiotensin-converting enzyme (ACE). By selection for antibody binding to endothelial cells of bovine rather than rat origin we have obtained a reagent that has broad cross-species binding properties and that can at the same time serve as a useful marker for the surface of endothelial cells. The IgM-producing clone that we have established, alpha-ACE 3.1.1, has been grown in ascites form to yield ascites fluid that binds selectively to immobilized ACE at a greater than 1:10,000 dilution. By use of enzyme-linked immunosorbent assays, immunofluorescence histology, and flow cytometry, we have demonstrated the presence of ACE on endothelial cells of murine, bovine, and human origin. By means of a fluorescence-activated cell sorter (FACS-IV) we have been able to selectively isolate viable endothelial cells from a mixture of endothelial cells and fibroblasts. We believe the antibody will be useful not only for the selection and in vitro cultivation of endothelial cells but also as a tool for the identification and pharmacological study of ACE.

Animals↗
PubMed1980

Peripheral 6-hydroxydopamine administration in the rabbit (Oryctolagus cuniculus): effects on Pavlovian conditioning.

The present experiment studied the effects of 6-hydroxydopamine (6-OHDA) administration and adrenal enucleation on differential classical conditioning of somatic and cardiovascular changes in the rabbit. Heart rate and blood pressure conditioned responses (CRs) were relatively large decreases in rate and slight depressor responses, respectively, which were both attenuated by 6-OHDA administration and adrenal demedullation. Frequency of eye-blink response to the reinforced conditioned stimulus was diminished by sympathetic interference during the middle sessions of conditioning, thus producing a decrement in eye-blink discrimination. Electromyographic CRs, on the other hand, were not affected by peripheral catecholamine reduction. These results are discussed in terms of the role of afferent autonomic feedback in influencing the acquisition of somatomotor behaviors.

Adrenal Medulla↗
PubMed1979

Adrenal demedullation and peripheral 6-OHDA administration in the rabbit: effects on body weight, general activity and cardiovascular responsivity.

Two experiments were undertaken to determine the effects of systemic 6-hydroxydopamine (6-OHDA) administration and adrenal enucleation on body weight, brain and heart norepinephrine, general activity, and cardiovascular responsivity in the rabbit. 6-Hydroxydopamine produced long lasting, dose related decreases in baseline blood pressure (BP) and in pressor responses following electric shock. Partial reversal of the BP changes was observed in nonenucleated animals tested 20 days after 6-OHDA administration but not in enucleated animals tested 5, 10 or 20 days post-injection or in control animals tested 5, 10 or 15 days after vehicle injection. Although 6-OHDA administration produced transient weight loss and activity decrements, no long term weight or activity changes were observed.

Adrenal Medulla↗
PubMed1975

Movements at the hip joint.

Movements at the hip joint are first discussed in terms of free movement of the lower limb on the trunk. The biomechanics of standing on one lower limb limited to the situation in the coronal plane are presented. The role of the hip muscles in the upright posture and in the movements at the hip joint during walking on the flat and upstairs and downstairs is described.

Female↗