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J Joly

Publications and source records attributed to J Joly.

At least 55 records · Page 3Linked to original sources
PubMed1985

Seasonal variations of androgens, estrogens, and progesterone in the different lobules of the testis and in the plasma of Salamandra salamandra.

Progesterone, 4-androstenedione, testosterone, dihydrotestosterone, 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol), 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol), estrone, and estradiol levels were determined by radioimmunoassay in the different lobules of the testis of Salamandra salamandra throughout the year according to the seasonal cycle. 3 beta-diol levels were not detectable. High levels of steroids were found in the grandular tissue (enlarged pericystic cells after spermiation) and large variations were showed for progesterone, 4-androstenedione, testosterone, 3 alpha-diol, and estrone. In the mature lobule (formed by cysts with mature spermatozoa), only testosterone showed seasonal variations and in the immature lobule (with early stages of meiosis), 3 alpha-diol showed fluctuations. The major estrogen found in the testis of Salamandra was estrone; estradiol stayed at a low level throughout the cycle. The steroids fluctuation seems to be related to the histological evolution of the testis throughout the cycle. The present data were the first on steroid seasonal variations in the testis of an urodele.

Androgens↗
PubMed1983

The dynamics of the steroidogenic response of perfused Xenopus testis explants to gonadotropins.

The dynamics of androgen production by perfused Xenopus testis explants has been investigated under various gonadotropic stimulations. Androgens were measured in perfusate by radioimmunoassay. The behavior of hormones in the perfusion system was assessed. Pieces of testis were capable of producing androgens after more than 20 hr in perifusion. Amplitude and duration of the response to a gonadotropic stimulation were the same 3 and 10 hr after the beginning of the experiment. After stimulation by pituitary extract, hCG, or carp GTH, there was a rapid tenfold increase in androgen secretion with a maximum after 1.5 hr and then a slow decrease to a basal line level. A typical log dose-response curve was obtained with hCG and cGTH. After 6 hr continuous stimulation by pituitary extract, steroid secretion increased and reached a plateau 4 hr later. The decrease occurred only after the end of stimulation. The same amount of pituitary extract injected into the column at the rate of 4 min stimulations every hr gave the same type of response. As far as is known, the present data are the first on perifusion systems on the testes of lower vertebrates.

Androgens↗
PubMed1983

Interactions of serotypes A and B of Candida albicans in mice.

Candida albicans serotypes A and B were mixed in different proportions and administered orally to white mice receiving a protein-free diet. Recoveries of the serotype from the stomach and rectosigmoid showed that the relative proportion of serotype A was consistently higher than in the inoculating suspension. No modification of serotype was observed in mice given only one serotype, and there was no change in proportions of serotypes coincubated in vitro. These observations indicate that host factors must be involved in selective colonization by the serotypes in vivo.

Animals↗
PubMed1983

[Precipitation reactions between secretory products in amphibian oviducts].

The existence of precipitin reactions between some molecules in egg jellies (oviduct secretions) of tailed amphibians (Amphibia caudata) has been demonstrated by double diffusion on agarose plates. These reactions do not exist in frogs and toads (Amphibia salientia). One precipitin reaction was related to compounds with a common molecular site of interaction for all A. caudata: all cross-species reactions were possible; a common antigenic rate has been shown. Another precipitin reaction, positively demonstrated in Pleurodeles waltl, probably exists in other A. caudata. The putative influence of these reactions on egg jelly-spermatozoon interactions has been discussed. An homology between these intra-egg jelly reactions and cortical granule content-egg jelly reactions in A. salientia has been suggested.

Amphibians↗
PubMed1981

[Anti-Candida albicans serology. A study carried out on 178 pregnant women in labour (author's transl)].

The serological study of 178 women investigated during labor shows that the number of patients with a significant titer of Candida albicans antibodies is 161 % higher in those suffering from vulvovaginitis due to this fungus as compared to patients without current infection. Among the 30 patients having vaginitis, 73 % harbor the yeast in the gastrointestinal tract. Precipitins were present in 6 patients : all of them also have a high titer of fluorescent antibodies. From the results of this work, it can be concluded that Candida albicans serology, although still difficult in its interpretation, is an interesting tool for the clinician and for the study of the physiopathology of candidosis.

Candidiasis↗
PubMed1980

Microbial flora associated with Candida albicans vulvovaginitis.

The relationship between vaginal microflora Candida albicans vulvovaginitis is studied. Results obtained from 340 vaginal specimens showed an increase of isolates in the absence of C albicans for all groups of microorganisms (168%), particularly for the gram-negative bacteria (226%). A high frequency of polymicrobial vaginitis is noted, as is the probable importance of anaerobic microorganisms. Finally, some special aspects of the pathogenesis of C albicans infection are discussed.

Candidiasis, Vulvovaginal↗
PubMed1980

alpha-1-Antitrypsin phenotypes in French Canadian newborns.

Cord blood sera from 390 French Canadian babies born consecutively in a Montreal hospital were phenotyped for alpha 1-antitrypsin. As in other populations, Pi M was the most frequent allele. The second most frequent was Pi S (0.0923). This Pi S allele frequency is significantly different from that of other Canadians, but similar to that of Normans and Bretons.

Canada↗
PubMed1980

[Amphibian jelly envelopes (proteins secreted by the oviduct) and their role in fertilization].

The jelly envelopes, surrounding amphibian eggs and playing a major part in the initial steps of fertilization, are deposited around the eggs as they traverse the different parts of the oviduct. These envelopes form 5 to 8 morphologically distinct layers according to the species, i.e. in Anura and Caudata they are organized differently, and are composed of about ten different macromolecular components (glycoproteins). The innermost layer is rich in sulfate and poor in fucose, a carbohydrate abundant in the other layers. This is due to a fucosyltransferase deficit in the cranial part of the oviduct secreting this layer. The cranial and the medial oviduct secretory products show a lectin carbohydrate-like reaction which is pH-dependent and can be inhibited by carbohydrates like glucuronolactone. This reaction has made it possible to isolate, by affinity chromatography, the main secretory component of the cranial oviduct (Spop). This paper discusses the main functions of the jelly layers of the egg, with particular reference to fertilization. The role of their macromolecular components in sperm capacitation is clear in Anura; experimental investigation in Urodeles is more difficult. We propose several hypotheses to explain the molecular interactions leading to capacitation. Block to polyspermy in amphibians is discussed.

Amphibians↗
PubMed1980

Stunted growth Candida albicans in human amniotic fluid in vitro.

The interaction of human AF with the pathogenic yeast Candida albicans was studied in vitro in 262 samples from early midtrimester and term gestations. Among 111 fluids collected between 15 and 18 weeks, 84% (93) manifested growth-inhibiting activity on 10(2) fungal cells/ml at 24 hr. This phenomenon persisted to a similar extent in late pregnancy. It could not be explained by a lack of nutrients in AF, since the addition of TSB failed to influence the proportion of active fluids. Depletion of the yeast cell glycogen reserve potentiated the degree of fungistasis achieved in several samples (p = 0.0009). Supplementation with either SDB or a physiologic concentration of carbon dioxide effected a complete reversal (p less than 0.0001). Preliminary data as well as overall results suggest that the absolute transferrin content and its pH-dependent saturation with iron are key factors invovled in the growth-inhibiting activity. Germ tube formation was unrestricted in 115 second trimester fluids studied. Human AF probably helps to prevent congenital candidiasis, and this protective function must be better defined.

Amniotic Fluid↗
PubMed1979

A study of 666 strains of Candida albicans: correlation between serotype and susceptibility to 5-fluorocytosine.

The minimal inhibitory concentrations (MICs) of 5-fluorocytosine (5-FC) and the serotypes were determined for 666 strains of Candida albicans isolated from white patients in Canada. Of these strains, 495 (74.3%) were serotype A and 71 (25.7%) were serotype B. Resistance to 5-FC (MIC, greater than or equal to 25 microgram/ml) was observed in 11.3% of the serotype A strains and in 49.7% of the strains of serotype B. Most specimens were from the urogenital and respiratory tracts. The results showed the difference between the distribution of the two serotypes of C. albicans in North America and that in Europe. Serotyping of C. albicans strains by indirect immunofluorescence is a rapid means of predicting the de novo sensitivity of this year to 5-FC. The specificity of this technique for the isolated strains was approximately 79%.

Animals↗
PubMed1978

[Myxedema heart].

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Heart Diseases↗