Search PubMed⌕ Search

Biomedical subjects

J Joffe

Publications and source records attributed to J Joffe.

25 records · Page 2Linked to original sources
PubMed1977

Histones H2a, H2b, H3, and H4 are present in equimolar amounts in chick erythroblasts.

A quantitative labeling method for stoichiometric analysis has been applied to an investigation of the histone composition of chick erythroblast chromatin. The necessity of long fractionation procedures and the dangers of preferential extraction and staining are eliminated through the use of this simple procedure which involves the direct analysis of nuclear proteins on sodium dodecyl sulfate gels. Only a knowledge of the amino acid compositions of the histones is required. H2a, H2b, H3, and H4 are found to be present in nearly equimolar quantities.

Animals↗
PubMed1976

The effect of maternal and fetal corticosteroids on the development of function of the pituitary-adrenocortical system.

The pituitary-adrenocortical system of rat fetuses was stimulated (larger adrenals at birth) by maternal adrenalectomy, or suppressed (smaller adrenals at birth) by implantation of an ACTH secreting pituitary tumor (MtTF4). Offspring were delivered by caesarean section and fostered to untreated females. Offspring of intact females delivered by caesarean section and normally delivered offspring of intact mothers both fostered to untreated lactating females served as controls. Body growth in the first three weeks of life was delayed in offspring of tumor bearing mothers in Control-fostered subjects as compared to the 2 other groups. At 70 days of age female offspring of the tumor implanted and adrenalectomized mothers, as well as the Control-caesarean females, had smaller adrenals than Control-fostered animals of the same sex. The adrenal size of males was not significantly affected. No significant differences were found in resting concentrations of corticosterone in plasma, although offspring of adrenalectomized mothers had high values. Suppressed adrenal response to ether stress was found in offspring of tumor bearing mothers. The supposition is that interference with maternal pituitary-adrenocortical activity during pregnancy has a long lasting effect on fetal hypothalamo-pituitary-adrenocortical system.

Adrenal Cortex Hormones↗
PubMedDate not supplied

Palliative resection for colorectal carcinoma.

In an attempt to identify those patients who would benefit from palliative resection of the colon or rectum and those who would not, an analysis of ten years of resections was undertaken. For the 81 patients who underwent palliative resection, the operative mortality was 10 per cent and the postoperative morbidity was 50 per cent. The mean survival was 11.4 months and the median survival nine months. Poor prognostic factors deduced were patients with extensive liver metastases, those over the age of 75 years, and a previous history of cardiovascular disease. It is believed that patients with a combination of these factors may not benefit from palliative resection. Otherwise, resections satisfactorily relieve patients of the symptoms of impending obstruction and rectal bleeding and are, therefore, believed to be worthwhile.

Adult↗