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Biomedical subjects

J Jeekel

Publications and source records attributed to J Jeekel.

At least 253 records · Page 14Linked to original sources
PubMed1980

Prolongation of canine renal allograft survival. A study on the effect of donor pretreatment.

Treatment of kidney donors with procarbazine hydrochloride and methylprednisolone, respectively, 5 and 2 1/2 hr before harvesting the kidney, improved renal allograft survival in dogs significantly. Pretreatment of the donor did not have a deleterious effect on the early function of the kidney grafts. Donor blood transfused peroperatively into the recipient caused a significant reduction in survival of kidney grafts from pretreated donors, although it did not influence the survival of nontreated kidneys. Furthermore, it appeared that a peroperative injection of a suspension of nonirradiated donor lymphocytes as well as donor lymphocytes irradiated with 2,500 rad significantly decreased the survival time of pretreated kidneys. A peroperative transfusion of leukocyte-poor blood prepared with a leukocyte filtration column, which leaves erythrocytes, thrombocytes, and plasma and eliminates most of the leukocytes (99.9%), also abolished the effect of donor pretreatment. Thus, administration of donor blood constituents, whether lymphocytes or leukocyte-poor blood, can abrogate the beneficial effect of donor pretreatment on kidney graft survival. These data indicate that the effect of donor lymphocytes on the survival of pretreated kidneys is not because of a specific immunological activity of these lymphocytes but merely because of the presence of antigens on their cell surface.

Animals↗
PubMed1980

Modification of kidney graft survival in dog and man by pre-operative transfusion to the donor.

The present experiments indicate that the transplantation reaction is not solely caused by immunocompetent cells of the recipient, but also by immunocompetent cells in the donor organ. Immunisation of the donor did modify the immune response as demonstrated with kidney grafts in rat, dog and man. In the dog prolonged kidney graft survival by one peroperative blood transfusion was reduced to control level by transfusion of the donor on day -1 with 100ml third party blood. In the rat third party blood transfusion to the donor reduced kidney graft survival significantly, but donor pretreatment with recipient lymphocytes induced significantly prolonged survival. This suggests that the modification of graft survival by donor transfusion is an immunological phenomenon. Immunisation of the donor with recipient cells may induce specific immunoreactive cells in the graft that causes a local graft versus host reaction, which inhibits the rejection reaction. In man 44 recipients were studied who only received blood peroperatively. Significantly impaired graft survival was noted if the donor was not transfused, resulting in 19% 3-month kidney function, versus 61% with transfused donors.

Animals↗
PubMed1979

[Recipient conditioning with beagle plasma and beagle liver: prolongation of kidney-graft function without preoperative lymphocytotoxic antibody formation].

Donorspecific recipient conditioning in the non-related beagle produces prolonged kidney-graft survival without any postoperative immunosuppression. Besides 2 donor blood injections on days--18 and--11 followed by a 6-day preoperative immunosuppressive pulse (Procarbazine, ATS) donor plasma and a donor liver preparation are equally active. Additional long-term low dose preoperative immunosuppression with azathioprine tends to strengthen the effect. Lymphocytotoxic antibody formation is avoided in the recipient where no whole blood is used.

Animals↗
PubMed1978

Effect of prior third-party blood transfusions on canine renal allograft survival.

The relationships between immune reactivity after blood transfusions, subsequent kidney allograft survival, and donor selection were studied in dogs. Animals with a high as well as low serological immune reactivity toward antigens contained in blood transfusion were observed. Genetic control of this reactivity or a linkage of this property to DLA, sex, or red blood cell markers inheritance was not apparent in the four beagle families studied. The two recipients with the lowest immune reactivity scores were also found to be the longest survivors after a DLA-mismatched kidney graft. Seven other recipients with higher scores rejected their DLA-mismatched kidneys as rapidly as did untransfused animals. Kidney graft survival was decreased in some recipients of DLA-identical kidneys (n = 5), presumably through sensitization for minor histocompatibility antigens. A normal or an increased survival time of DLA-identical kidneys was found in the remaining animals (n = 6). The majority of these recipients appeared to have a higher than average reactivity in two-stage microcytotoxicity testing. This might have been attributable to the presence of enhancing antibodies. Further studies in preclinical animal models are needed to define the optimal transfusion policy for human patients awaiting a kidney graft.

Animals↗
PubMed1975

The effect of donor blood on renal allograft survival in DL-A tissue typed beagle littermates.

A single transfusion of 200 ml of donor blood 14 days before renal transplantation in prospectively DL-A tissue typed beagle littermates appeared to have an effect on graft survival. Seventeen per cent of the recipients did respond to the transfusion with formation of lymphocytotoxic and haemagglutinating antibodies. These "responder dogs" rejected kidney grafts in an accelerated way, compared with the "nonresponders" and with the nontreated control dogs. Responsiveness appeared to occur in pairs of littermates, which suggests that responding potency is genetically determined. There was histological evidence of acute arteritis in the renal grafts of responders, whereas cell-mediated rejection was noted in nonresponders.

Animals↗
PubMed1975

Effect of anti-donor serum (ADS) and donor cells on renal allograft survival in DL-A tissue-typed littermate beagles.

The influence of passive and active immunization on kidney allograft survival was tested in littermate beagles differing in one and two DL-A haplotypes. Immunization by treatment of recipients with hyperimmune antidonor serum (ADS) prolonged graft survival only in a few donor-recipient combinations differing in one DL-A haplotype. All ADS batches that were tested in MLC reaction did block this reaction, but no correlation with its in vivo activities was found. Hyperimmunization of recipients with donor lymphoid cells before grafting caused a second-set rejection, whereas one injection with donor blood did so only in 14%, with antibody production and accelerated rejection. A short course of horse antidog lymphocyte serum (HADLS) prolonged kidney graft survival. The addition of intravenously given donor bone-marrow cells to this protocol did not lead to a further increase in graft survival.

Animals↗
PubMedDate not supplied

Colorectal cancer recurrence and perioperative blood transfusions: a critical reappraisal.

The last 2 decades the immunomodulatory effect of blood transfusions has been investigated intensively. The effect of blood transfusions on the prognosis of colorectal cancer patients is reviewed in this paper. We made an evaluation of the material from animal and from clinical studies present in the literature. The results from clinical randomized trials dealing with this subject, which have been published recently, are discussed as well.

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