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Biomedical subjects

J Jansen

Publications and source records attributed to J Jansen.

At least 271 records · Page 15Linked to original sources
PubMed1982

[The effects of postparturient anthelmintic treatment on ewes and their lambs (author's transl)].

Anthelmintic treatment of ewes after lambing resulted in partial suppression of the spring rise in egg counts in Frisian milch-sheep, lambing early in March and a rather satisfactory suppression of the spring rise in Texel ewes lambing late in March. The Frisian and Texel sheep were treated with 44 mg/kg and 66 mg/kg thiabendazole respectively. Beneficial effects of treatment were not observed in the ewes or their lambs compared with the controls. Particularly, treatment failed to produce any improvement of the gain in weight in the lambs. In the Frisian ewes, the haemoglobin content was determined at regular intervals. Both in the treated and in the untreated group, the haemoglobin content decreased significantly. Moreover, this decrease was correlated with the increase in faecal Haemonchus egg counts These results and those of previous experiments in conjunction with findings reported in the literature, lead to the general conclusion that farm management is highly decisive in answering the question as to how serious trichostrongylid and strongylid infections will be and the extent to which anthelmintic treatment of the ewes could have a beneficial effect.

Animals↗
PubMed1982

[Observations on the spring rise of faecal strongyle egg counts in goats (author's transl)].

Examination of the faeces at regular intervals during the period from February to June 1980 in a group of multiparous dairy goats produced the following results: (a) a marked increase in faecal strongyle egg-counts, (b) Haemonchus contortus was the worm mainly causing this increase, (c) differences in faecal egg-counts between Dutch white (Saanen) goats and Toggenburger goats or between groups of goats of different ages were not observed, (d) no differences in faecal egg-counts were observed in groups of goats kidding at different times. Therefore the increase in egg-counts was apparently not affected by parturition. The general conclusion is that a genuine spring rise rather than a peri- or post-parturient rise was observed in the goats. The striking difference between this increase in faecal egg-counts in goats and that in sheep, which is associated with parturition and lactation, is discussed.

Animals↗
PubMed1982

Serum inhibitors in aplastic anaemia.

To study the frequency and clinical importance of serum factors inhibiting CFU-c in patients with aplastic anaemia, sera from 21 patients were analysed. The sera of eight patients showed inhibition of at least one normal bone marrow, but strong and consistent inhibition of two or more normal bone-marrow samples was found in only two cases. In both of these two patients the inhibition disappeared after successful therapy. Fair, but not complete, correlation was found between serum inhibitors and HLA-antibodies, suggesting that at least part of these inhibitors are antibodies directed against HLA-antigens present on myeloid progenitor cells. Serum inhibitors appear to be infrequent in the population of patients with aplastic anaemia seen in our hospital and appear to have no major implications for therapy or outcome of the disease.

Adolescent↗
PubMed1982

The individual variation in pharmacokinetics and pharmacodynamics of furosemide in young normal male subjects.

Following a 24 h control period in the ward 80 mg furosemide was injected intravenously to ten young healthy, male volunteers. The serum clearance of furosemide (Cls) was between 140 and 201 ml min-1 and on the average the renal clearance was 60% of Cls. During the initial 30 min period a maximum additional excretion rate of sodium of 3.3 mmol min-1 was reached at an excretion rate of 0.8 mg furosemide min-1. A marked initial drop in creatinine clearance (Clcr) was noted and Clcr(24 h) showed an average decrease of 12% after the drug administration. The serum concentration of potassium was decreased at 1 and 2 h after the injection and of sodium from 2 h and on. The concentration of albumin in serum increased by 3% (P less than 0.05) already after 5 min. After 2 h a maximum increase of 14% was reached. After 8 min diastolic blood pressure was increased by 13% (P less than 0.05), whereas systolic blood pressure reached a significant decrease gradually (7% after 3 h).

Adult↗
PubMed1982

Anti-thymocyte globulin treatment for aplastic anemia.

20 patients with severe aplastic anemia were treated with anti-thymocyte globulin (ATG), 6 of them in combination with haplo-identical bone marrow. 7 patients (35%) showed a good clinical response within 6 months; they were off transfusions and had greater than or equal to 0.8 x 10(9)/l neutrophils. ATG had the greatest effect on red-cell production and the least on platelet production. The hematological recovery with ATG could not be predicted from the bone-marrow histology, CFU-c growth, or clinical data. However, patients with strong HLA antibodies seemed to respond more often. The actuarial survival was 55% at 5 years. Under intensive supportive care, even 7 out of 12 non-responders were alive after 1 year. ATG appears to be a useful form of therapy for patients with severe aplastic anemia who are not candidates for bone-marrow transplantation.

Adolescent↗
PubMed1982

Artificial periodontal defects in beagle dogs. A clinical evaluation during 24 months.

Artificial periodontal defects were evaluated clinically for 24 months in six beagle dogs. Defects were created bilaterally around the lower second, third and fourth premolars. The gingiva was separated from the teeth by intrasulcular incisions and copper bands were cemented around the teeth as far apically as possible. After 3 weeks the bands were replaced by cotton ligatures, which were kept in place deep subgingivally for 11 weeks. The dogs were placed on a soft diet, allowing plaque accumulation throughout the study. One week after removal of the ligatures, probing depths of around 4.5 mm were recorded. During the evaluation period of 24 months, plaque accumulation was abundant and subsequently the gingiva was heavily inflamed. However, a distinct healing tendency was evident clinically. Two years after removal of the ligatures, probing depth was reduced to approximately 3.5 mm and the attachment level as assessed clinically had changed in a coronal direction. Part of this recorded increase in periodontal attachment may have been the result of changes in composition and condition of the periodontal tissues resulting in differences in probing depths.

Animals↗
PubMed1982

The phenotype of the neoplastic cells of hairy cell leukemia studied with monoclonal antibodies.

Eighteen cases of hairy cell leukemia were studied with a battery of polyclonal anti-Ig and nine monoclonal antibodies to determine the lineage of the hairy cells (HC) and the stage of their maturation arrest. Hairy cells tend to nonspecifically bind many antisera and precautions had to be taken to avoid nonspecific fluorescence of the cells. All but one case was reactive with one light chain type and one or more heavy chain isotype antisera as reported before. All cases studied were positive for monomorphic HLA-DR determinants, using monoclonal antibody 7.2. Most cases tested (6/7) were positive with the B-lineage related antibody PI 153/3. While most cases (15/18) were nonreactive with the B-lineage related antibody BA-1, they became positive (5/5) following in vitro culture. Seven out of nine cases were reactive with OKM1. Common acute lymphoblastic leukemia antigen (CALLA) was absent in all (15) cases tested and the ALL associated structure p24/BA-2 was absent from 16 of 18 cases. HC from none of the cases were clearly positive with the T-cell antibodies 9.6., or TA-1, whereas in only 1/18 the cells reacted with T101. The results of this study support the B cell lineage of most HC, and show the presence of multiple phenotypes. In combination with the surface Ib present on the cells, a hierarchy of phenotypes is postulated, with SIg-ormu delta, BA-1+, PI 153/3+, HLA-DR+ being the most immature, and SIg(alpha) gamma, BA-1-, PI 153/3-, HLA-DR+ the most mature.

Antibodies, Monoclonal↗
PubMed1982

Monoclonal antibody BA-1 does not bind to hematopoietic precursor cells.

Monoclonal antibody BA-1 binds to B lymphocytes, to cells from most cases of non-T acute lymphoblastic leukemia (ALL), and weakly to neutrophils. To determine whether BA-1 also reacts with hematopoietic progenitor cells (HPC), we studied the effect of removal of BA-1+ cells from human bone marrow on the proliferation in vitro of the trilineage precursor cell CFU-GEMM, and on the committed progenitor cells of granulopoiesis (CFU-C) and erythropoiesis (BFU-E/CFU-E). Complement-mediated cytotoxicity using BA-1 at concentrations far beyond those required to lyse BA-1+ bone marrow cells and ALL cells did not result in inhibition of colony formation in any of the assays. A rosette separation method, using ox red blood cells coated with BA-1, resulted in enrichment of HPC in the BA-1-depleted interface, whereas very few HPC were found in the BA-1-enriched pellet. Both methods indicate that BA-1 does not bind to HPC, although binding of the antibody to the lymphohematopoietic stem cell cannot be excluded yet. The high cytotoxic capacity of the IgM antibody BA-1, and the lack of reactivity with HPC, make the antibody particularly suitable for use in autologous bone marrow transplantation for patients with ALL.

Adolescent↗
PubMed1982

Cell markers in hairy cell leukemia studied in cells from 51 patients.

To determine the maturation arrest of the neoplastic cells of hairy-cell leukemia (HCL) and the spectrum of the surface markers on these cells, a series of 51 patients with this disease was studied. The cells of all but two of the patients showed monoclonal surface Ig with respect to light chains. In about one-third of the cases, only gamma heavy chain determinants were present on the cells; the majority carried multiple heavy chain determinants as documented by the application of different fluorochromes. Two patients each showed two different clones of cells, both of the same light chain type. In one of these two patients, two paraproteins were present in the serum. Intracytoplasmic Ig was found in only 4 of 39 cases, in all instances being IgM. All cases studied concerned cells with FclgG receptors; however, the density of this receptor varied. FcIgM receptors also showed a spectrum of density, with some cases showing very few FcIgM-positive cells. Receptors C3 were not observed on the hairy cells. Serum immunoglobulin levels were normal or increased. Paraproteins were found in the sera of 4 of 38 patients. These data suggest that HCL is a neoplasm of B lymphocytes. The neoplastic cells are probably arrested at a more mature stage than the cells of chronic lymphocytic leukemia. The multiple isotypes on the cells indicate a block at the "switch" phase from the small micro-carrying lymphocyte to the larger Ig-producing lymphocyte or plasma cell.

Adult↗
PubMed1982

Prognosis in infantile hydrocephalus. Follow-up in adult patients, born 1946-1955.

Twenty-six patients with infantile hydrocephalus were re-examined at ages ranging from 21-32 years. Operations had been performed in 11, but abnormal head growth continued post-operatively in 9. One third of the patients lead a normal life, while half of the patients are mentally deficient. All patients have neurological signs, poor dexterity and clumsiness being present in all. Psychological testing reveals uneven profiles, in 4 patients with performance IQ considerably lower than verbal IQ. Computerised tomography demonstrates all degrees of hydrocephalus with no correlation between ventricular-brain ratio and IQ or between ventricular-brain ratio and operative procedures. Full otoneurological investigation shows differential-caloric examination to be abnormal in 60%, although none of the patients have complaints referring to the vestibular system.

Adult↗
PubMed1982

Population build up of gastrointestinal nematode infections in ewes and lambs on pasture grazed by calves in the previous year.

The development of trichostrongylid infections was studied in ewes, lambs and tracer lambs grazing a pasture which has been used by calves in the previous year. Treatment with fenbendazole of the ewes two days before they were turned out to pasture virtually prevented infections with trichostrongylid species characteristic for sheep. Significant burdens of Ostertagia leptospicularis, Cooperia oncophora and Nematodirus helvetianus were found. An increased pasture infectivity level compared to spring was observed in C oncophora and O leptospicularis in August and September. The possibility that O leptospicularis may build up to pathogenic levels in sheep and cattle as a result of alternate grazing is discussed. The two other species are probably of little significance as pathogens in sheep.

Animals↗
PubMed1982

Clinical staging system for hairy-cell leukemia.

To find a clinical staging system for patients with hairy cell leukemia, 391 patients contributed by 22 centers were analyzed using the proportional hazard survival model. Attention was paid to nonsplenectomized patients to find a staging system to predict the survival length at the time of diagnosis. On the basis of hemoglobin level and spleen size at the time of diagnosis, 3 stages could be distinguished with significantly different prognoses (stages I-III). In addition, we addressed the question of splenectomy to identify those patients who benefit from the operation. Using arbitrary, but clinically relevant, criteria to call the operation beneficial, splenectomy appears to be indicated for patients with large spleens (greater than or equal to 4 cm under costal margin) or with smaller, but palpable, spleens when anemia (Hb less than 12 g/dl) is present. The third question concerned the splenectomized patients. To indicate the patients who have a poor postsplenectomy survival, a staging system was developed on the basis of hemoglobin level and number of neutrophils at 2-3 mo after the operation (stages A-C). The validity of the two staging systems was supported by the results of an analysis of an independent test series of patients. These staging systems may be helpful for the choice of therapy and in the planning of clinical trials in patients with hairy-cell leukemia.

Actuarial Analysis↗
PubMed1982

Normal human pluripotential and committed hematopoietic progenitors do not express the p24 antigen detected by monoclonal antibody BA-2: implications for immunotherapy of lymphocytic leukemia.

Analysis of surface antigenic determinants of hematopoietic progenitor cells has relevance both to basic biologic study of cell differentiation and to potential clinical application in the diagnosis and treatment of hematologic neoplasia. The production and characterization of monoclonal antibody BA-2 by immunization with a pre-B-ALL cell line has been reported previously. In this study we utilized complement-dependent cytotoxicity and rosette-separation with antibody indirectly coupled to ox RBC to determine if the antigen (p24) recognized by the antibody BA-2 is represented on human pluripotential (CFU-GEMM) or committed hematopoietic progenitors (CFU-GM, BFU-E, CFU-E). BA-2 showed no reactivity with normal hematopoietic progenitors by either method. In contrast, BA-2 exhibited potent complement-mediated cytotoxicity for selected ALL-derived cell lines. These results show that normal human hematopoietic progenitors do no express antigenic sites represented on ALL cells that are recognized by BA-2 and suggest that this monoclonal antibody may serve as a potent and specific agent for treatment of lymphocytic leukemia, perhaps most useful in ex vivo marrow conditioning for autologous bone marrow transplantation.

Adolescent↗