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J J Jones

Publications and source records attributed to J J Jones.

At least 109 records · Page 6Linked to original sources
PubMed1971

Plasma-renin assay.

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Biological Assay↗
PubMed1969

The clearance and antidiuretic potency of neurohypophysial hormones in man, and their plasma binding and stability.

1. Bio-assay techniques have been used to measure plasma levels of neurohypophysial hormones in man, following either a single injection or a continuous infusion.2. The median half-life of oxytocin after a single injection of 2 u. was 3.2 min (2.0-5.7, 95% confidence limits); this increased significantly (P < 0.01) to 4.8 min (4.4-6.1) when the hormone was infused at a rate of 500 m-u./min. The vasopressins had appreciably longer half-lives. After a single injection of 1 or 1.5 u. 8-lysine vasopressin (LVP), the half-life was 5.7 min (3.6-6.0). Continuous infusions of the hormones at a rate of 120 m-u./min yielded half-lives of 5.5 min (5.0-7.1) for LVP, and 5.6 min (3.9-9.5) for 8-arginine vasopressin (AVP).3. The apparent volumes of distribution of the hormones were all of the order of two thirds the extracellular volume.4. In accordance with its shorter half-life, the clearance of oxytocin was greater than that of the vasopressins (1.5 l./min, compared with 1.0 l./min).5. The antidiuretic potencies of the hormones were studied in over-hydrated subjects, by measuring the rate of urine excretion following an I.V. injection. Duration of antidiuretic action increased in the order: oxytocin, LVP, AVP. A 5:1 mixture of oxytocin and AVP was not as long-lasting as AVP alone. 8.5% (4-22) of an administered dose of AVP was excreted in the urine, and this amount was significantly correlated with urine volume (r = +0.67, P < 0.05).6. Ultrafiltration of human plasma containing exogenous hormones showed that 30% (13-50) of AVP was bound, the degree of binding being independent of concentration over the range used (50-400 mu-u./ml.) In contrast, oxytocin was completely unbound.7. Exogenous oxytocin was more stable than exogenous AVP in human plasma. At 4 degrees C there was no significant loss of oxytocin until 7 days, whereas 20% of AVP was inactivated in 2 days. At 37 degrees C a 20% loss of AVP occurred within 4 hr, and a 50% loss within 24 hr; corresponding times for oxytocin were 24 and 48 hr.

Adult↗
PubMed1968

Factors influencing the sensitivity of the rat to vasopressin.

1. Indirect evidence suggests that the concentration of arginine vasopressin in the plasma of normally hydrated man is about 1 mu-u./ml., but this is usually considered to be below the limit of sensitivity of the standard assay preparation, the water-loaded Wistar rat under ethanol anaesthesia.2. It was found that there was a surprising variation in sensitivity to vasopressin between batches of Wistar rats, and that other varieties of rat (including those with diabetes insipidus) were no more sensitive.3. Three modifications of the standard assay procedure produced an increase in sensitivity:(a) using Wistar rats weighing 100-150 g, rather than larger animals;(b) commencing the assay shortly after surgery, i.e. as soon as the urine flow reached 25 mul./min;(c) infusing vasopressin intravenously (0.5-3 mu-u./min). By using modification (a) with either (b) or (c) it was possible to detect as little as 0.5 mu-u.4. With these modifications antidiuretic activity equivalent to 0.5-2.0 mu-u./ml. of arginine vasopressin was measured in nine samples of plasma from a normally hydrated subject.5. It is suggested that the frequent reports of enhanced sensitivity may have been due to the fortuitous use of a particularly sensitive batch of rats, or to a high endogenous secretion of vasopressin due to operative trauma.

Animals↗