Biomedical subjects

J J Calvo

Publications and source records attributed to J J Calvo.

At least 37 records · Page 2Linked to original sources

PubMed1992

Effect of intraduodenal sodium bicarbonate in rat and rabbit exocrine pancreatic secretion.

The effect of intraduodenal sodium bicarbonate, 0.1 M, on exocrine pancreatic secretion and the release of two peptides, secretin and VIP, was studied in anesthetized rats and rabbits, two species largely used in the gastroenterology laboratories. In the rabbit, intraduodenal sodium bicarbonate perfusion had no effect either on exocrine pancreatic secretion or on portal plasma levels of secretin and VIP. By contrast, in the rat, intraduodenal sodium bicarbonate perfusion significantly increased hydroelectrolyte exocrine pancreatic secretion and portal plasma secretin levels. A clear interspecific difference reflecting the different gastrointestinal physiology of both species is observed.

Animals↗

PubMed1992

Cholinergic mechanisms involved in the effect of intraduodenal ethanol on exocrine pancreatic secretion.

The effect of intraduodenal ethanol on exocrine pancreas of the rat in basal conditions has been studied. The pancreatic flow rate significantly increased to a maximum of 148% of basal after intraduodenal ethanol whereas only slight increases of protein output were observed. Ethanol perfusion in previously atropinized animals also elicited a significant increase of the flow rate up to a maximum of 177% of basal values but this increase appeared later than that observed in non-atropinized animals. Our results show that the ethanol action is successively stimulant followed by a simultaneously dual and antagonic effect (stimulant and inhibitory). Furthermore, this ethanol effect is mediated by cholinergic mechanisms. Finally, no effect of ethanol on portal plasma secretin and VIP levels was observed in atropinized or non-atropinized animals.

Animals↗

PubMed1991

Pancreatic dose dependent effect of intraduodenal HCl in the anesthetized rabbit.

1. A dose-response relationship between intraduodenal perfusion of different HCl loads (1.8, 4.5, 9, 18 and 45 mmol/hr) and volume, bicarbonate and protein outputs, from the exocrine pancreas of anaesthetized rabbits was observed. 2. This study also shows a dose-response relationship between the different HCl loads and the porta levels of secretin and VIP. 3. The secretin response showed a marked decrease during the stimulation period and this may suggest the involvement of a neural mechanism. 4. The plasma VIP responses were of a magnitude that might suggest a hormone role for VIP in this effect.

Animals↗

PubMed1990

Acinar cholecystokinin (CCK) receptors in the exocrine pancreas of the rat: effect of adrenalectomy.

The binding of [125I]BH-CCK-8 to membranes of acinar cells from rats at 6 and 21 days after adrenalectomy was studied. The optimum conditions of time and temperature were previously established as being 120 min and 30 degrees C. Under these conditions, the membranes of the adrenalectomized animals of both groups (6 and 21 days) bound more radioligand than those from control rats. However, a qualitative study of the binding showed that the affinities of binding were much lower; in particular, the high affinity receptors had a Kd of 0.94 +/- 0.33 nM in the controls and this was 14.9 +/- 1.29 nM in the 6-day adrenalectomized animals, although the maximum binding capacity did not vary significantly. However, in the case of the low affinity receptors, there was a gradual increase in the maximum binding capacity as the time after adrenalectomy progressed: 717 +/- 121, 1,987 +/- 183, and 10,175 +/- 862 fmol/mg for the control, 6-day, and 21-day adrenalectomized rats, respectively. In the latter situation, the high affinity receptors completely disappeared. These results, which coincide with a marked deficit in protein secretion already described in adrenalectomized rats, can be accounted for in terms of the possible negative cooperativity exerted by the low affinity receptors on the high affinity hormone-receptor complex, the protein secretion of the acinar cells normally mediated by the high affinity receptors becoming paralyzed.

Adrenalectomy↗

PubMed1990

Duodenal alkalinization releases secretin and vasoactive intestinal polypeptide and stimulates exocrine pancreatic secretion in the anesthetized rat.

The effect of various intraduodenal alkaline solutions (0.1 M NaHCO3, 0.1 M Na2CO3 and 0.025 M NaOH) on exocrine pancreatic secretion and the release of two peptides, secretin and vasoactive intestinal polypeptide, was studied in anesthetized rats. The flow rate of the pancreatic juice was stimulated up to a maximum of 179, 158 and 180% and the protein output up to 181, 131 and 162% (compared with basal) after duodenal perfusion of, respectively, 0.1 M NaHCO3, 0.1 M Na2CO3 and 0.025 M NaOH. Maximum increases in portal plasma secretin concentrations of 143, 146 and 190% and maximum increases in VIP of 116, 155 and 147% after, respectively, intraduodenal 0.1 M NaHCO3, 0.1 M Na2CO3, and 0.025 M NaOH were found. In conclusion duodenal alkalinization in the rat produces a pancreatic exocrine secretory response that may be partially ascribed to the effect of secretin and VIP.

Animals↗

PubMed1990

Effect of ACTH on VIP and galanin release from the pituitary.

The effect of adrenocorticotropin (ACTH) on the release of four regulatory peptides from the anterior pituitary of male rats has been studied using an in vitro perfusion system. Quartered anterior pituitaries from male adult Wistar rats were perfused with buffer containing different concentrations of ACTH and, subsequently, 56 mM KCl. Fractions of 1.5 ml were collected at 3 min intervals and analyzed for vasoactive intestinal peptide (VIP), galanin, 7B2, and substance P, using specific radioimmunoassays. Concentrations of 0.02, 0.1, 0.2, and 0.4 microM ACTH produced increases of 117 +/- 50%, 155 +/- 90%, 163 +/- 14%, and 161 +/- 3% (mean + SE), respectively, of basal release of VIP (P less than 0.001). However, concentrations of 1 microM and 2 microM ACTH suppressed VIP release to 74 +/- 6% and 47 +/- 4%, respectively, compared to basal release (P less than 0.001). Results for galanin release were similar: concentrations of 0.02, 0.1, 0.2, and 0.4 microM ACTH increased galanin release to 129 +/- 4%, 136 +/- 8%, 143 +/- 9%, and 133 +/- 9% of basal release (P less than 0.001) and 1 and 2 microM ACTH provoked a suppression of 52 +/- 7% and 50 +/- 13%, respectively, compared with basal release (P less than 0.001). Doses of ACTH that altered the secretion of VIP and galanin had no effect on 7B2 and substance P release. These results demonstrate that ACTH causes a release of pituitary VIP and galanin in vitro and, moreover, that this is a biphasic phenomenon.

Adrenocorticotropic Hormone↗

PubMed1990

Release of substance P from rat hypothalamus and pituitary by endothelin.

Endothelin-1 is a 21 amino acid peptide originally isolated from porcine aortic endothelium and has recently been localized within the central nervous system. We have administered endothelin-1 in a dynamic perfusion system in order to study its possible effects on the rat hypothalamus and anterior pituitary. Tissue (hypothalami or quartered pituitaries) was placed into plastic chambers and was perfused with oxygenated Krebs-bicarbonate solution. After an interval to establish stable basal peptide release, endothelin-1 was administered at two doses (0.1 and 1 microM) and the release of substance P, vasoactive intestinal peptide, 7B2, and somatostatin was measured, the last being detectable only in hypothalamic perfusates. Both concentrations of endothelin-1 led to a significant increase (P less than 0.01) in the release of substance P from the hypothalamus and pituitary, but not of vasoactive intestinal peptide, 7B2, or somatostatin. Thus after the 0.1 microM and 1 microM endothelin-1 perfusion substance P release from the hypothalamus increased by 125 +/- 5% and 215 +/- 15% (mean +/- SEM) of basal and from the pituitary by 168 +/- 8% and 276 +/- 15% (mean +/- SEM). No change occurred in the output of ACTH or other pituitary hormones. The release of substance P from hypothalamus or pituitary after stimulation with endothelin-1 was not blocked when a calcium free medium was used. Endothelin-1 binding sites were identified on rat pituitary cell membranes. These findings suggest the possibility that endothelin may act as a paracrine substance, neurotransmitter, or neuromodulator in the hypothalamo-pituitary axis.

Animals↗

PubMed1990

Caerulein-induced acute pancreatitis in the rat. Pancreatic secretory response to cholecystokinin.

The response of pancreatic exocrine secretion to cholecystokinin (CCK), has been studied in experimental acute pancreatitis induced in rats by supramaximal doses of caerulein. Several doses of caerulein were used (4, 20 and 40 micrograms/Kg) and each one was administered by four subcutaneous injections over 3 h at hourly intervals. Pancreatic juice was collected 9 h after the first injection. The caerulein-treated animals showed a statistically significant increase in serum amylase levels. Secretory activity of ductular cells remained unchanged in all the caerulein-treated animals, but total protein and amylase secretion decreased significantly at all the caerulein doses used, both in resting conditions and under stimulation with CCK (1.25 micrograms/Kg/h). Despite this the acinar cells of rats treated with the lowest dose of caerulein retained a certain degree of secretory function since amylase activity in pancreatic juice was greater than in other groups of rats treated with higher doses of caerulein. Moreover, the percentage of increase observed in total protein and amylase in response to CCK respect to basal secretion is similar to that of the untreated animals. At higher doses (20 and 40 micrograms/Kg) the secretory capacity in response to CCK was inhibited. Therefore CCK administration in slight acute pancreatitis could be used as a therapy since it favours the secretion of pancreatic enzymes at percentual levels similar to those of the controls.

Acute Disease↗

PubMed1990

Glucocorticoids effects on exocrine pancreatic secretion in caerulein-induced acute pancreatitis in the rat.

The present work reports on exocrine pancreatic secretion in control rats, adrenalectomized rats and hydrocortisone-treated (10 mg/Kg/d) rats during 7 days, under normal conditions and after induction of acute pancreatitis with caerulein (20 micrograms/Kg) by 4 subcutaneous injections at hourly intervals. Pancreatic secretion was seen to be affected by the procedure of adrenalectomy, which led to a marked reduction in the secretion of proteins and amylase with respect to control values. This was probably due to the decrease occurring in the zymogen granules in the acinar cells of the exocrine pancreas, a phenomenon which also led to a decrease in pancreatic weight observed in these animals. Treatment with hydrocortisone induced a decrease in the secretion of proteins and amylase, as well as an increase in pancreatic weight. This agrees with the accepted hypothesis that large amounts glucocorticoids stimulate the synthesis and storage of proteins in the exocrine pancreas, reducing the secretory phase. The administration of high doses of caerulein under these conditions led to acute pancreatitis in the three groups of animals. This was paralleled by a dramatic decrease in protein and amylase secretion and by severe interstitial edema of the pancreas and by increases in serum amylase values. In the case of the animals treated previously with hydrocortisone, the latter were tripled with respect to the control animals. The conclusion is offered that since the storage of enzyme proteins is governed by glucocorticoids, which furthermore increase the sensitivity of the acinar cells to stimulation by secretagogues, the administration of these substances during the development of pancreatic lesions such as acute pancreatitis is highly compromising to the organism.

Adrenalectomy↗

PubMed1989

Beta 1 and beta 2 adrenergic agonists in exocrine pancreatic secretion in the rabbit.

The effect of Dobutamine (a beta 1-adrenergic agonist) and Terbutaline (a beta 2-adrenergic agonist) on exocrine pancreatic secretion was studied in anaesthetized rabbits, simultaneously controlling pancreatic blood flow and blood pressure. The secretion of fluid and ions (bicarbonate, sodium and potassium) was unaffected by the infusion of Dobutamine (8 micrograms.kg-1.min-1) or Terbutaline (10 micrograms.kg-1.min-1). Neither were pancreatic blood flow or mean blood pressure altered. Dobutamine or Terbutaline depress the function of the acinar cells, amylase secretion being more affected by the action of Terbutaline. The results show that beta 1 and beta 2-adrenergic stimulation has no effect on the ductular cells but does decrease the secretion by the acinar cells.

Adrenergic beta-Agonists↗

PubMed1989

Alpha-adrenergic influences on exocrine pancreatic secretion in the rabbit.

Action of phenylephrine (35 micrograms/Kg/min) alone or previously blocked by phentolamine (100 micrograms/Kg/min) on exocrine pancreatic secretion of anaesthetized rabbits has been studied, in basal state or under stimulation by secretin (1 C.U./Kg/h) or by the octapeptide of cholecystokinin (OP-CCK) (0.15 Ivy dog units/Kg/h). Phenylephrine increased arterial pressure. This effect was blocked by phentolamine. However no variations were seen in pancreatic blood flow in any of the experimental conditions assayed. Phenylephrine produced a secretin-like effect on hydroelectrolytic secretion in basal conditions. This action was maintained after the infusion of secretin but not after OP-CCK. This effect was not blocked by phentolamine. Phenylephrine increased protein secretion in the basal state, an action that was blocked by phentolamine. After secretin or OP-CCK stimulation phenylephrine did not increase protein secretion. It is concluded that phentolamine blocks the effects of phenylephrine on acinar cells but not on ductular cells.

Animals↗

PubMed1989

Exocrine pancreatic response to isoproterenol in the rabbit under secretin and CCK stimulation.

The effect of isoproterenol (0.3 micrograms/Kg/min) on exocrine pancreatic secretion stimulated by secretin (1. C.U./Kg/h) and the octapeptide of cholecystokinin (OP-CCK 0.15 Ivy dog units/Kg/h) was studied in anaesthetized rabbits. Pancreatic blood flow remained unaffected by the action of isoproterenol; however, the administration of the beta-adrenergic agonist over an infusion of secretion an OP-CCK led to an increase in pancreatic juice flow (approximately 146% for secretin and 125% for OP-CCK) and HCO3-output (approximately 194% for secretin and 150% for OP-CCK), a potentiation being observed between the effects of secretin and isoproterenol. This kind of response, independent of vascular changes, is due to a direct action of isoproterenol on the ductular cells. Isoproterenol interferes with the action of secretin and OP-CCK in that it partially inhibits the stimulatory action of OP-CCK on the enzymatic secretion of the acinar cells in the rabbit pancreas and at the same time protein secretion decreases in the presence of secretin.

Acid-Base Equilibrium↗

PubMed1989

Plasma ferritin of sows during pregnancy and lactation.

We studied a set of indicators of iron metabolism in sows and new-born piglets: hemoglobin, plasma iron, total iron binding capacity and plasma ferritin. Iron status of seventy-two sows was studied throughout pregnancy and lactation. The results point to mobilization of iron from its storage sites up to the second month of gestation, but no appreciable decrease during the second half of pregnancy. No appreciable mobilization of the iron stores of sows was observed during the lactation period. Nine sows and their 78 piglets were used to compare the status of iron in the sows and the end of gestation with the iron status of the piglets at birth; there was a positive correlation between the mean values of plasma ferritin concentration in the piglets and the levels of plasma ferritin in their mothers.

Animals↗

PubMed1988

Alterations of pancreatic juice amylase by glucocorticoid levels in the rat.

By use of isoelectrofocusing, three isoenzymes with pIs of 8.40, 8.55, and 8.65 were characterized in the amylase fraction of rat pancreatic juice. Enzyme secretion in rat exocrine pancreas is affected by glucocorticoid levels; adrenalectomy led to a significant decrease in protein secretion which was more pronounced in the amylase fraction, in which the isoenzymes with pI 8.55 and 8.65 disappeared. Substitution therapy with hydrocortisone (25 mg/kg/day, for 6 days) restored exocrine pancreatic secretion to almost normal levels. Administration of hydrocortisone to control rats led to structural alterations in enzymes secreted, splitting the amylase isoenzymes with pI 8.40; this was confirmed by crossed immunoelectrophoresis. It is concluded that glucocorticoid levels play an important role in the maintenance of function of exocrine pancreas and it is suggested that, although hydrocortisone fulfills the objective of restoring enzyme secretion diminished by adrenalectomy, it is possible that intensive treatment could have undesirable effects on the structure of enzymes and could involve pancreatic disfunctionality.

Adrenalectomy↗

PubMed1988

Influence of glucocorticoids on the secretion of pancreatic juice in the rat.

The influence of adrenalectomy and hydrocortisone treatment on the exocrine pancreatic secretion has been studied in anaesthetized rats. In the adrenalectomized animals Na+ administered in the saline solution provided for drinking was able to maintain standard sodium levels in serum. In these animals an increase of Na+ secretion in pancreatic juice was observed. Furthermore, the osmotic effect created by the increase in Na+ would account for the increase in pancreatic flow. In these adrenalectomized rats, an increase in K+ output is observed, which can be explained by the high K+ concentrations in serum. Likewise adrenalectomy decreased pancreatic enzyme secretion and produced a loss in weight of the organ that is accounted for by a lack of glucocorticoids. Hydrocortisone administration did not affect neither the secretion nor the weight of the pancreas of the control rats but the hormone proved to be effective in adrenalectomized rats producing a pancreatic secretion close to normal, balancing the secretory rate of water, Na+ and K+, completely restoring total protein secretion and the weight of the pancreas but amylase secretion in part only. It is therefore concluded that the weight of the pancreas and its exocrine secretion are clearly influenced by adrenalectomy and by substitution therapy with hydrocortisone. The administration of this hormone (25 mg.kg-1.day-1 along 6 days) did not affect intact animals.

Adrenal Glands↗

PubMed1986

Plasma ferritin and other parameters related to iron metabolism in piglets.

The evolution of mean plasma ferritin values, hematocrit, hemoglobin, plasma iron concentration and total plasma iron binding capacity were studied during the growth of piglets from 0 to 50 days. The results obtained point to a massive mobilization of iron from storage sites during the second and third weeks of life of these animals. Apart from plasma ferritin values and the total plasma iron binding capacity, the coefficient of utilization may be considered as another parameter to be taken into account upon evaluating iron deposits in piglets.

Aging↗

PubMed1986

Ox spleen ferritin: an isoelectrofocusing and crossed immunoelectrofocusing study.

Ox spleen ferritin was purified and its purity checked by two-dimensional immunoelectrophoresis and polyacrylamide plate electrophoresis. Microheterogeneity was shown with a preparation of purified ferritin by isoelectric focusing. The protein was separated into at least 6 fractions; two large fractions in the 4.50-4.55 pH range and another 4 in the 4.65-4.80 interval. Microheterogeneity was confirmed in purified preparations by crossed immunoelectrofocusing. Seven fractions were observed, the most acid ones (4.50-4.55) also being the most abundant. In the crossed IEF procedure, exactness in the isoelectrophoretic separation time is important in that excessive time may impair the resolution potential.

Animals↗

PubMed1983

[Variations in plasma ferritin during the transition from immature hens to mature ones in relation to laying].

The mobilization of iron stores during the transit from non laying to laying hen has been studied. A series of parameters related to iron metabolism (hemoglobin, hematocrit, plasma iron, transport iron binding capacity and plasma ferritin) have been determined during six consecutive weeks in which the setting percentage increased from 0 to 83%. Five male samples had been previously estrogenized to obtain a quick answer for the same parameters determined in the females. A decrease in plasma ferritin levels has been observed in both experiments, suggesting a mobilization of iron stores to the egg especially during the two weeks in which the setting percentage increase was highest (16-60%).

Animals↗