Search PubMed⌕ Search

Biomedical subjects

J Hyland

Publications and source records attributed to J Hyland.

50 records · Page 3Linked to original sources
PubMed1980

The bacteriological environment of preterm infants.

Bacteria were cultured in the intra-uterine environment in 72% of 56 preterm infants studied. Placental tissue yielded the greatest number of positive cultures, with anaerobic organisms accounting for one-quater of these. Bacterial growth was obtained in the specimens of 4 out of 5 infants who died, and in 5 clinically infected babies. The incidence of bacterial infection was not increased by rupture of membranes for more than 18 hours, by amniocentesis or by administration of dexamethasone to the mother.

Bacteria↗
PubMed1980

Faecal bile acid excretion in diverticular disease.

Faecal bile acid excretion and sigmoid myoelectrical activity were measured on three separate occasions over a 12-month period in 21 patients with diverticular disease and the result were compared with those of 17 age-matched normal subjects. Statistically significantly lower faecal concentrations of both deoxycholic and lithocholic acid were found in patients with diverticular disease (96.7 +/- 11.3 mg/100 g, 59.6 +/- 6.0 mg/100 g respectively) when compared with normal subjects (219 +/- 32.0 mg/100 g and 117.6 +/- 14.6 mg/100 g respectively, P < 0.001). These values persisted throughout the period of study. Patients with diverticular disease were found to have an abnormally rapid myoelectrical rhythm (frequency 0.2-0.3 Hz [12-18 c/min] which returned towards normal after 1 year's bran treatment. Significant correlations were found between lithocholic acid concentrations and activity of 0.2-0.3 Hz (r = 0.67) and deoxycholic acid concentrations and activity of 0.1-015 Hz (6-9 c/min) (r = 0.62). It is suggested that increased colonic absorption of bile acids may occur in diverticular disease which may influence the myoelectrical activity.

Bile Acids and Salts↗
PubMed1980

Changes in myoelectrical activity in the irritable colon syndrome with prolonged treatment.

Twenty patients with established irritable colon syndrome were studied at presentation and following a mean period of 14.3 months of bran treatment. An abnormal myoelectrical activity consisting of a high incidence of 3 c/m slow-wave electrical activity was recognised in the rectosigmoid and confirmed by objective automated frequency analysis. After the period of prolonged treatment, the high 3 c/m slow-wave activity persisted, but the incidence of 6--9 c/m activity increased. This was associated with an improved stool weight and transit time in patients with predominant constipation but no alteration in patients with predominant diarrhoea.

Colonic Diseases, Functional↗
PubMed1979

The effect of bile acids on colonic myoelectrical activity.

The effect of different concentrations of sodium cholate, sodium chenodeoxycholate and sodium deoxycholate on colonic myoelectrical activity was studied in 4 dogs. There was no statistically significant difference in percentage motility, motility index, basal pressure or mean slow wave frequency with either cholic or chenodeoxycholic acid (5--15 mM). Deoxycholic acid (15 mM), however, caused a marked increase in percentage motility (median control value 4.1, range 0--23; after installation, median value 27--0, range 20.6--45.0, P less than 0.001). This was accompanied by an increase in spike activity, but no alteration in mean slow wave frequency or basal pressure was seen. Cholestyramine inhibited the motor response. It appears that only deoxycholic acid affects both motility and absorption, presumably by different pathways, and may thus assume importance in disorders of colonic motility.

Action Potentials↗
PubMed1977

A multicentre study of the susceptibility of a variety of bacteria to cephalothin, cefamandole, tobramycin and gentamicin.

A multicentre study of antibiotic susceptibility was performed in South Africa. Sensitivity to cephalothin, cefamandole, tobramycin and gentamicin was tested on a variety of aerobic and anaerobic bacteria. Two disc susceptibility techniques were used, i.e. the Kirby-Bauer technique (aerobes) and the broth-disc method (anaerobes); minimum inhibitory concentrations (MICs) were determined according to the International Collaborative Study techniques, and regression lines for individual centres were constructed. Satisfactory lines were obtained for cephalosporins, but, in some centres, problems were experienced with the aminoglycosides. Variations in MICs for Haemophilus influenzae were probably due to an inoculum effect. Accumulative percentage tables of the number of strains inhibited were compiled, and the comparative performance of the antibiotics was assessed.

Anti-Bacterial Agents↗
PubMed1977

The use of counter-immuno-electrophoresis to identify causative organisms in bacterial meningitis: experience in Cape Town.

Crebrospinal fluid (CSF) from 142 patients was tested for the presence of Haemophilus influenzae, pneumococcal and meningococcal antigens by counter-immuno-electrophoresis with commercial antisera. Group- or type-specific antigen was detected in the CSF of 67% of 64 patients with meningitis proved by culture to be due to these organisms, and in 10 of 25 patients with purulent meningitis but negative cultures. No false positive results were obtained in 24 normal CSF specimens, or in the CSF of 29 patients with meningitis caused by other organisms. The diagnostic usefulness of this specific, relatively simple and rapid procedure is confirmed.

Antigens, Bacterial↗
PubMed1977

Temperature-sensitive variants for saturation density and anchorage dependencey of a simian virus 40-transformed human X mouse hybrid cell line.

Several variant clones temperature sensitive for some parameters of transformation from a hybrid cell line containing a stable diploid mouse genome and two human chromosomes 7 carrying an integrated defective simian virus 40 have been isolated. Like the wild-type (wt) cells, at the permissive temperature the temperature-sensitive (ts) clones grew to high saturation densities and readily formed colonies in methyl cellulose. In contrast to the wt cells, at the nonpermissive temperature they had variable but always lower saturation densities and were unable to form colonies in methyl cellulose. The fraction of cells that synthesized DNA decreased at both temperatures when the cells reached saturation density, but it represented always at least 20% of the total population. All of the ts clones so far tested had a near triploid chromosome number, contained from one to three human chromosomes 7, and were T-antigen-positive both at the permissive and nonpermissive temperatures. The ts clones maintained low saturation density at the nonpermissive temperature because of a decrease in the number of cells that were actively proliferating and because of the shedding of cells into the medium. Temperature downshifts and refeeding allowed for expression of the permissive phenotype. In most of the isolated clones anchorage independency was not correlated with unrestricted cell proliferation. These variant clones may provide useful systems for a better understanding of the number of interdependent pathways involved in the expression of the phenotype of a transformed cell and elucidate the molecular mechanism required for the maintenance of a normal state in a cell population.

Antigens, Viral↗
PubMed1975

The role of angiography in the management of intestinal carcinoid.

Carcinoid tumors of the intestine may present with bowel obstruction or with the carcinoid syndrome. Confirmation of the diagnosis by biochemical means is easy. Management is often surgical and a number of different procedures have been advocated, e.g. resection of the primary, hemicolectomy and lately, partial hepatectomy. The angiographic features of two cases are presented and discussed; the usefulness of the information so gleaned in the choice of management is stressed.

Adult↗
PubMedDate not supplied

Early results with restorative proctocolectomy.

The first 25 restorative proctocolectomies (RP) performed by one surgeon since 1986 are reviewed. Ulcerative colitis (23) and idiopathic megarectum (2) were the indications for surgery. The initial seven patients had a submucosal proctectomy and transanal hand sewn pouch-anal anastomosis. Eighteen patients had a totally stapled RP. One patient with malignancy died from factors unrelated to surgery. Complications developed in eight patients after pouch-anal anastomosis and in four patients after ileostomy closure. There was one pouch failure due to pelvic sepsis. No pelvic sepsis has occurred following a stapled ileal pouch-anal anastomosis with defunctioning ileostomy. After a mean follow up period of 20.5 months all patients are continent (mean stool frequency = 4/day and 0.5/night). Soiling occurred in three patients who had a transanal hand sewn pouch-anal anastomosis. All patients in the stapled group have satisfactory control. Restorative proctocolectomy produces satisfactory operative and functional results which have improved as our experience has increased.

Adolescent↗