Search PubMed⌕ Search

Biomedical subjects

J Hustin

Publications and source records attributed to J Hustin.

126 records · Page 7Linked to original sources
PubMedDate not supplied

Immunofluorescent localization of placental lactogen, chorionic gonadotrophin and its alpha and beta subunits in organ cultures of human placenta.

Localization of human placental lactogen (HPL), chorionic gonadotrophin (HCG) and its free alpha and beta subunits in mature and immature placental villi before and during organ culture was examined with an 'indirect' immunofluorescent technique using highly specific antisera. HPL fluorescence was strictly localized to the syncytiotrophoblast and the intensity of this fluorescence increased with gestational age and decreased with the time of culture. Undissociated HCG and HCG beta immunofluorescence was localized to the syncytiotrophoblast. Maximum intensity was observed in immature placentae and was not significantly affected by the duration of culture. However, irregular and patchy HCG and HCG beta immunofluorescence was seen in the cytotrophoblasts under conditions of extensive syncytiotrophoblastic damage. HCG alpha immunofluorescence was localized in the syncytiotrophoblast of immature placentae and was more intense in mature placentae. Beginning the third day of culture, HCG alpha fluorescence increased and was also present in the cytotrophoblast. On the basis of these observations and additional data, the possibility is discussed that cytotrophoblastic cells, better preserved than the syncytiotrophoblast in case of restricted energy and oxygen supply, may actively synthesize free HCG alpha, in addition to syncytiotrophoblastic production of this subunit. By contrast, HPL, undissociated HCG, and HCG beta are mainly or exclusively eleborated in the syncytiotrophoblastic layer.

Animals↗
PubMedDate not supplied

Giant congenital melanocytic nevus with placental involvement: long-term follow-up of a case and review of the literature.

We describe a case of giant congenital melanocytic nevus with placental villous involvement in an otherwise uncomplicated pregnancy. Only four similar cases have been reported in the world literature. Nevomelanocytes were found in both villous stoma and fetal capillaries. No neonatal or maternal adverse effects were observed 5 years after diagnosis in the present case.

Adult↗
PubMedDate not supplied

Morphology and DNA content of endometrial cancer nuclei under progestogen treatment.

Twelve endometrial cancers have been observed before and during progestogen treatment. Eight were well-differentiated and displayed an objective response. It was essentially characterized by a volumetric reduction of the nucleus and the disappearance of tetraploid nuclei. The DNA content remained diploid or near-diploid. Reduction of nuclear non DNA proteins under hormonal treatment is suggested.

Cell Nucleus↗
PubMedDate not supplied

Cytologic evaluation of the effect of various estrogens given in postmenopause.

The cytologic evaluations of the hormonal (mainly estrogenic) treatment of 263 postmenopausal women are presented. Though estriol has a considerably weaker action than estradiol derivatives (and especially ethinyl estradiol) it has to be recommended for the oral treatment of local changes due to atrophy since this steroid is effective at the vaginal level in doses three to five times smaller than those necessary to induce endometrial proliferation.

Administration, Oral↗
PubMedDate not supplied

[Value of examining the placenta in pregnancies complicated by fetal malformations].

This investigation was undertaken to determine whether examination of the placenta contributed to a better understanding of the causes of fetal malformations. Specific histological abnormalities were found in triploidy and lysosomal storage disorders. Placentas from trisomies only showed a marked a specific retardation of villous maturation. The most frequent placental abnormalities detailed in cases presenting major fetal malformations with normal karyotype were: velamentous insertion of the umbilical cord, placental hypotrophy, single umbilical artery, marginal retroplacental hematoma and generalized perivillous fibrin deposition. Examination of the placenta after fetal malformation can provide helpful informations in counseling the parents and the perinatal team in the follow-up of any future child bearing.

Congenital Abnormalities↗
PubMedDate not supplied

[Germ cell tumour and pseudohermaphroditism. Histological and cytogenetic study (author's transl)].

The case report is that of a 15 years old boy presenting with unilateral undescended testis and hypospadias. Surgical exploration disclosed an absence of regression of the Müllerian system and an intra-abdominal gonad replaced by a seminoma. There are arguments to suspect that the seminoma arose from a gonadoblastoma controlateral testis biopsy disclosed a germ cell abnormality consistent with an in situ neoplastic transformation. It is emphasized that all forms of cryptorchidism must be explored surgically and that apparently normal gonads left in place in cases of unilateral gonadic dysgenesis, must be attended with extreme care.

Adolescent↗
PubMedDate not supplied

[Origin and sites of action of inhibin (author's transl)].

Mice testis in organ culture (31 degrees C) produce a steroid free substance which inhibits FSH secretion by rat pituitary cells. Histologically, Sterloti cells alone remain normal after a few days in culture. A hypothalamic action of inhibin is demonstrated in vitro. The intraglandular content in LHRH is inversely related to the amount of inhibin present in the culture medium. In the testis, DNA synthesis is depended of inhibin. This is demonstrated by studies of the incorporation of tritiated thymidine in testicular DNA in vivo and in vitro. In conclusion, inhibin is produced by Sertoli cells and acts not only at this pituitary level but also on the hypothalamus and the testis.

Animals↗
PubMedDate not supplied

Cytologic patterns of reparative processes, true dysplasia and carcinoma of the gastric mucosa.

The cytologic features of dysplasia and carcinoma of the stomach are still poorly defined. In a series of 90 cases, a precise correlation could be made between smears and histologic data. Cytology was sensitive enough to allow characterization of: (1) active repair/regeneration, associated, in a number of cases, with intestinal metaplasia (slight dysplasia); the cellular atypia is minimal; (2) true dysplasia (formerly moderate and severe); this preneoplastic state is characterized by cellular atypia within clusters; and (3) true carcinomas (intestinal or diffuse type); classic criteria for malignancy are present, with a prominent lack of cell cohesiveness, though distinction from dysplasia may be very difficult. The advantages of cytology are the survey of large mucosal areas, investigation of the cardial region, not always accessible to endoscopic biopsy, and exploration of large ulcers. With the adjunct of cytology, the specificity and sensitivity of endoscopy plus histology clearly increase.

Biopsy↗