Search PubMed⌕ Search

Biomedical subjects

J Hurych

Publications and source records attributed to J Hurych.

At least 55 records · Page 3Linked to original sources
PubMed1977

Relations of pulmonary diffusing capacity to ventilation and haemodynamics in healthy subjects.

The pulmonary diffusing capacity (DLCO) was measured in 13 healthy subjects during heart catheterization by the steady-state method (according to Bates and his coworkers). The DLCO shows the closest correlation to the tidal volume at rest, and to the central blood volume during exercise. Further, the DLCO correlates with the cardiac index both at rest and during exercise and with the stroke index at rest only. The DLCO did not show any relationship to the pressures in central circulation, pulmonary vascular resistance, total and alveolar ventilation. Statistical analysis shows that DLCO is most consistently related to the tidal volume at rest, whereas relation to central blood volume is much more stable during exercise.

Adolescent↗
PubMed1977

Predictability of arterial pH and PCO2 from right atrial blood samples in experimental animal.

Arterial and right atrial blood samples were drawn simultaneously under acutely changing acid-base and ventilatory conditions in a series of experiments carried out in nine pigs. The pH, PCO2, bicarbonate concentration and base excess from arterial and right atrial blood are compared. The predictability of the arterial values from the right atrial values is discussed. It is concluded that right atrial blood samples are approximately as informative as arterial blood samples for determining the acid-base status under both normal and acutely changed acid-base and ventilatory conditions.

Animals↗
PubMed1976

Present state of cardiovascular community control programme in the Czech Socialist Republic.

The high incidence, great import, and long duration of cardiovascular diseases are reflected in high demands placed on the health services. Experience shows that utilization of the results of research in general practice is lagging behind. The application of any improvement in the diagnosis, therapy, and prevention in health care waits several years for its accomplishment. In order to improve this situation, the Ministry of Health of the CSR constituted, in line with WHO recommendations, a Department for Cardiovascular Diseases Control. The Department has worked out a programme of prevention and control of the major cardiovascular diseases, in particular, ischaemic heart disease, systemic hypertension and its complications, rheumatic heart disease, congenital cardiac and vascular defects, and cor pulmonale. New diagnostic, therapeutic, and preventive procedures are first tried out in so-called model areas and are only after this introduced into the national health care of people suffering from or endangered by cardiovascular diseases. In parallel, organizational measures necessary for comprehensive care are implemented. The authors report on the experience gained so far with the realization of the programme of care of people suffering from IHD and acute myocardial infarction. They emphasize the importance of continual schooling of medical personnel and of health education of the entire population. They describe the implementation on a national scale of postgraduate cardiological courses intended especially for first-line doctors.

Cardiovascular Diseases↗
PubMed1975

Creatinephosphokinase in human diploid cell lines.

The activity of CPK was proved in cultivated cells from amniotic fluid cells, thymus, spleen, pericardium, umbilical cord, skin and muscle. The activity in these strains did not significantly differ. The average value is 224 plus or minus 68 i.u. CPK/mg N. No differences of activity were disclosed between embryonal, foetal and children cultures. The activity of CPK in post mortem cultures was not altered. The relationship between collagen synthesis and CPK activity is suggested. The evidence of CPK activity in amniotic fluid cells corresponding to the activity in other foetal cultures and the whole sample under investigation opens the way to the prenatal investigation of pathophysiology and inborn errors of the metabolism of this enzyme.

Amniotic Fluid↗
PubMed1975

Biosynthesis of collagen during the life cycle of human diploid cell lines.

In the course of the active phase of growth collagen synthesis appears relatively stable and decreases when the culture passes into the degenerative phase III. The increase of cell nitrogen indicates the increasing accumulation of cell proteins with their aging in vitro. The ratio of soluble to insoluble collagen is relatively constant in fibroblast cultures from different tissues, periods of development during the active phase of growth. The increase of insoluble collagen in long term cultivated Marfan syndrome fibroblasts indicates the possible relationship between increasing stability of collagen and ageing of fibroblasts in vitro. Further studies confirmed the significant differences between control fibroblast and fibroblast derived from tissues of patients affected by Marfan syndrome. No differences were found in collagen synthesis and in the ratio of soluble to insoluble collagen in fibroblast strains from embryos, foetuses, children and young adults. The collagen synthesis and the ratio of soluble to insoluble collagen does not differ in fibroblast cultures derived from or post-natal period of life. The collagen synthesis in amniotic fluid cells was confirmed by analysis of cell proteins and proteins produced into the cultivation medium. The ultrastructural analysis of these cells reveals formation of intracellular fibrills and their growth in the extracellular space. The possibility of prenatal investigation of inborn errors of collagen metabolism is pointed out.

Adrenal Glands↗
PubMed1975

Effect of hyperbarism on collagenous protein metabolism in granulation tissue.

Rats with subcutaneously implanted polyurethane sponges were exposed 6 hours daily for 7 days to high ambient atmospheric pressures (1.5, 2, 2.5 and 3 ATA). Another group was exposed 4 hours daily for 4 weeks to 3 ATA before inducing granulation tissue formation. 14C-proline was administered 16 hours before terminating the experiment. Free hydroxyproline, soluble and insoluble collagen and total noncollagenous protein were isolated from the 7-day granuloma and the amount and radioactivity of 14C-hydroxyproline and 14C-proline were determined. Seven days' graduated hyperbarism did not affect collagen synthesis; the maturation of collagen to insoluble forms was inhibited at 2 and 2.5 ATA, but not at 3 ATA. Stimulated degradation of collagen (free hydroxyproline) was observed at 2, 2.5 and 3 ATA. In animals subjected to long-term exposure at 3 ATA pressure, the collagen in the granuloma matured to insoluble forms more quickly. Biochemical changes were correlated with changes in the fine structure of the granulation tissue. The appearance of the fibroblast proteosynthetic apparatus was not influenced by hyperbarism. Progressive spherical transformation, fusion of mitochondria and lysosomal activation in the pericapillary fibroblasts occurred at 2, 2.5 and 3 ATA. In short-term experiment, the formation of cytosegresomes and cellular necrosis also contributed to the effect at 3 ATA, which is thus already a toxic pressure for granulation tissue.

Animals↗