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Biomedical subjects

J Hurlimann

Publications and source records attributed to J Hurlimann.

54 records · Page 3Linked to original sources
PubMed1980

[Histologic diagnosis of lymphoproliferative diseases of the digestive tract].

The pathologic diagnosis of lymphoproliferative diseases of the gastrointestinal tract is sometimes very difficult or even impossible. In the majority of cases the gross appearance of the lesion is not helpful. Three histological criteria, when present, justify a definite diagnosis: cellular anaplasia and nodal involvement in malignant lymphomas, and the presence of germinal centers in pseudolymphomas. All other histological criteria, including transmural extension of the lesions, are of only relative diagnostic value. The definite diagnosis of these diseases is based on histological examination of the entire lesion. Only a diagnosis of probability is generally possible on endoscopic biopsies. The demonstration of different chains of immunoglobulins by means of immunohistochemistry may help in revealing malignant lymphomas with secretory activity and a polyclonal pattern in reactive lesions.

Biopsy↗
PubMed1978

Immunoglobulins and secretory component in endometrium and cervix. Influence of inflammation and carcinoma.

The synthesis of immunoglobulins and secretory component in the cervix and the endometrium was studied by tissue culture and immunofluorescence. Out of the 75 cervical biopsies studied, 17 were epidermoid carcinomas, 8 were carcinomas in situ and 23 tissues had inflammatory or metaplastic lesions. A total of 49 samples from endometrium were studied, out of which 22 were in the proliferative phase, 17 were in the secretory phase and 4 were carcinomas. In the cervical tissues without lesions, there were very few plasmacytes, the synthesis of immunoglobulins was low and in 66% of the tissues the synthesis of IgG was equal to or higher than that of IgA. With local modifications, the IgG synthesis was even more preponderant and was very important in epidermoid carcinomas which were infiltrated with numerous IgG plasmacytes. Secretory component was synthesized by almost all the tissues except the epidermoid carcinomas. The endometrium did not synthesize immunoglobulins; secretory component was synthesized only by endometrial tissue in the secretory phase and by 2 of the 4 carcinomas studied. It seems that in the cervix and the endometrium there is no relationship between the production of secretory component and the presence of IgA plasmacytes which probably localise as a result of other influences. The conditions in which the local secretory immunological system would react preferentially remain to be determined.

Autoradiography↗
PubMed1978

Antigens of a human breast carcinoma cell line (BT 20). I. Synthesis of serum proteins, membrane-associated antigens, and oncofetal-associated antigens.

The synthesis of various products by a human mammary cell line (BT 20) was studied by incorporation of 14C-labeled amino acids, choline, glucosamine, or galactosamine into nondialyzable materials. These products had molecular weights ranging from less than 12,300 daltons to more than 200,000 daltons. They were analyzed by immunoelectrophoresis and double diffusion in agar. Among the synthesized products, the following proteins were identified: beta2-glycoprotein I, alpha2HS-glycoprotein, alpha2-lipoprotein, actin, beta2-microglobulin, carcinoembryonic antigen, three oncofetal-associated antigens, and various erythrocyte membrane-associated antigens (namely, glycophorin). Synthesis of milk proteins was not detectable. Only the protein moiety of the glycophorin molecule seemed to be synthesized. The beta2-microglobulin was synthesized in an unbound state as well as bound to a glycoprotein whose relationship with the transplantation or tumor antigens must be determined. The three oncofetal-associated antigens were also synthesized in vitro by human fetal tissues and neoplastic and dysplastic human mammary tissues.

Actins↗
PubMed1976

In vitro synthesis of immunoglobulins and other proteins by dysplastic and neoplastic human mammary tissues.

The synthesis of several proteins in human mammary carcinomas and in dysplastic breast tissues was studied by tissue culture and by immunofluorescence. The synthesis of immunoglobulins showed marked quantitative and qualitative variations from one specimen to another, and a preferential synthesis of immunoglobulin G and C'3 in the carcinomas with lymphocytic infiltration. Fixation of immunoglobulins G and M on the surface of neoplastic cells was noted in some carcinomas. Secretory component was detectable in some cases by immunofluorescence, but no synthesis could be found in vitro, presumably because of unfavorable culture conditions. The synthesis of lactoferrin, casein, and some serum alpha- and beta-globulins was significantly greater in noncancerous tissues than in carcinomas. Synthesis of lactoferrin was also more frequent in the well differentiated carcinomas than in the poorly differentiated carcinomas. The tissue culture technique used in this study, although in need of better adaptation to the requirements in vitro of human mammary tissues, proved to be a useful tool for investigating the synthesis of several protein components by the epithelial cells of cancerous and dysplastic tissues of the human breast. Whether a preferential synthesis of 1 class of immunoglobulins or of other proteins might influence the evolution of a mammary lesion could not be determined in this material but it should be studied further.

Alpha-Globulins↗
PubMed1976

Local immunization in the mammary glands of the rabbit.

The effects of a local immunization with bovine serum albumin in the left mammary glands of the rabbit have been compared with those of an intramuscular (i.m.) immunization. The ability of several tissues to synthesize antibodies was studied in vitro with or without in vitro antigenic stimulation, and the antibody class in the plasmocytes of these tissues was determined by immunofluorescence. After i.m. immunization, the serum antibodies synthesized by the spleen and the lymph nodes pass into the milk by transudation; there is no antibody synthesis in the mammary glands. After immunization in the left mammary glands, there is antibody synthesis in the left mammary glands and also in the spleen and lymph nodes. In the mammary glands in the antibodies belong essentially to the IgG class and are synthesized by the plasmocytes that infiltrate the granulation tissue around the injection sites. The fragments of the immunized glands are capable of an in vitro secondary response analogous to that made by the spleen and lymph nodes. Thus the local immunization of the mammary glands does not stimulate the secretory immunologic system but elicits an antibody synthesis in the granuloma as in any other tissue. This local immunization also provokes a traffic of sensitized cells to the same lymphoid tissues as an i.m. immunization does.

Animals↗
PubMed1975

Immunoreactive ACTH and cortisol plasma levels during pregnancy. Detection and partial purification of corticotrophin-like placental hormone: the human chorionic corticotrophin (HCC).

The high plasma cortisol and ACTH levels present in pregnant women as well as the non-parrallelism of their plasma extract dilution curves in comparison with the standard curve in the ACTH radioimmunoassay, are evidence for the presence of an ACTH-like substance during pregnancy which would interfere with the assay. Placental extracts were obtained by acid-acetone extraction, followed by partial purification with oxycellulose and by extraction with porous glass powder. A substance was detected which partially cross-reacted with synthetic human ACTH in the radioimmunoassay and which showed biological activity using the assay procedure described by Liscomb & Nelson. The data sustain the existence of an ACTH-like placental hormone: human chorionic corticotrophin (HCC).

Adrenocorticotropic Hormone↗
PubMed1966

The liver as the site of C-reactive protein formation.

The site of formation of C-reactive protein (CxRP, CRP) has been studied with tissues from rabbits, monkeys, and human beings. Rabbits and monkeys were stimulated to produce the acute phase protein by injection of turpentine, croton oil, endotoxin, paratyphoid-typhoid vaccine, or pneumococci. C(14)-amino acid incorporation in vitro was demonstrated by means of autoradiography of immunoelectrophoretic patterns made with culture fluids. It was found that among many different tissues tested liver was the only tissue which incorporated C(14)-lysine and isoleucine into CxRP or CRP. Only livers taken 16 to 24 hr after various stimuli were active; livers from normal animals or from animals killed 3 to 9 hr after stimulation did not produce detectable amounts of CxRP. Inflamed muscle from the injection site did not show C(14)-amino acid incorporation into CxRP. Several human tissues were also cultured, and a few liver cultures found to contain labeled CRP. The formation of CxRP or CRP by the liver was always accompanied by enhanced C(14)-amino acid incorporation into other serum proteins, but the reverse was not always found.

Amino Acids↗