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J Hubert

Publications and source records attributed to J Hubert.

At least 109 records · Page 6Linked to original sources
PubMed1995

Epstein-Barr virus lymphoproliferative disease of donor origin after kidney transplantation: a case report.

A case of an Epstein-Barr virus (EBV)-associated B lymphoproliferative disorder presented as a renal transplant obstruction is reported. The diagnosis was made from histology, immunohistochemistry, and EBV expression studies. Cytogenetic analysis showed the tumor to be of donor origin and revealed chromosomal translocation 46, XY, inv (1)(p35; q41), involving the EBV insertion site 1(1p35) and transforming growth factor beta 2(1q41) loci.

Aged↗
PubMed1995

Studies on multispecific resistance of gastrointestinal nematodes to benzimidazoles on dairy-goat farms.

Multispecific resistance to benzimidazoles was studied in three selected farms. These farms had bred dairy goats for more than 15 years. The helminths were introduced with the goats at the establishment of the farms which afterwards remained isolated. Nematode resistance could then be related to their own management practices. Faecal egg count tests and egg hatch assays were performed to assess intensity of resistance. The generic (infective larvae in faecal cultures) and specific richness (adult worms) were assessed. The resistant species were Trichostrongylus colubriformis, Teladorsagia circumcincta, Haemonchus contortus and Oesophagostomum venulosum. Faecal egg count reduction tests and egg-hatch assays did not match exactly. Faecal larval counts after treatments gave a distorted picture of multispecific resistance: Haemonchus and Oesophagostomum were very largely over represented. The number of species found in the three farms was relatively low compared with other reports in goat farms of the area. This reduction of diversity might also be due in part to characteristics of breeding management and history (use of permanent pasture and introduction of goats at the establishment of farm).

Animal Husbandry↗
PubMed1995

Role of the kidney in the production of a low molecular weight growth factor (MW < 1000 Da): experimental study in the pig.

Small peptide molecules known as low molecular weight growth factor (LMW-GF) have been identified in human serum. They enhance the effect of IGF-1 (insulin-like growth factor) on proteoglycan synthesis. In the present work we investigated the role played by the kidney in the production of LMW-GF, using the pig as an experimental model. Six pigs underwent bilateral nephrectomy followed 24 h later by orthotopic autotransplantation of the kidney. Renal and liver functions were evaluated by measurement of serum creatinine, urea, electrolytes, amino transferases (ASAT, ALAT), proteins, and bilirubin. LMW-GF was measured by bioassay using 11-day-old pelvic chick embryo cartilages. We observed that LMW-GF quickly disappeared from pig serum after nephrectomy and only reappeared when transplantation was successful. Reappearance of LMW-GF can precede improvement of renal function evaluated by plasma creatinine levels. These data appear to demonstrate that the kidney is involved in LMW-GF production.

Acute Kidney Injury↗
PubMed1995

[Bilateral testicular ischemia in vasculitis. Differential diagnosis with torsion and the value of color Doppler ultrasonography].

The authors report a case of acute bilateral testicular pain simulating simultaneous bilateral torsion of the spermatic cord in a 22-year-old man. Duplex ultrasound revealed parenchymal ischaemia secondary to an early form of polyarteritis nodosa (PAN). Surgery, performed because of the doubtful differential diagnosis with torsion of the spermatic cord, excluded this hypothesis. The aetiological investigation, after excluding an infectious cause, consisted of revealing at least three diagnostic criteria necessary for the diagnosis of PAN among those defined by Godeau Guillevin or the American College of Rheumatology. The authors also discuss the value of duplex ultrasound in the follow-up of this disease.

Adult↗
PubMed1995

[Metastasis of renal adenocarcinoma to the spermatic cord and the epididymis: 2 cases].

Metastases of renal cell carcinoma are exceptional in the spermatic cord and epididymis (17 cases reported in the literature). The authors report two cases of metastases occurring 30 and 44 months after radical nephrectomy, respectively. Both patients are alive without metastases after surgical resection, with a follow-up of 4 and 8 years after the diagnosis of renal cancer, respectively. The mechanism of development, usually retrograde venous spread, and the particular features of these metastatic sites are discussed.

Adenocarcinoma↗
PubMed1995

[A rare cause of neurologic bladder: arteriovenous dural fistula with venous epidural drainage].

Spinal dural arteriovenous fistula (DAVF) with epidural drainage is infrequent: 22 cases of the 194 reported DAVF. In the case reviewed, DAVF is associated with a complete urinary retention and its somatic neurologic features are moderate. Such spinal arteriovenous fistula clinical picture is rare and occurs in only 4 to 5% cases. Urological diagnosis approach is interesting because of a patient presenting with a benign prostatic hypertrophy.

Aged↗
PubMed1995

[Gangrene of the external genitalia].

The term gangrene of the male external genital organs is applied to two different entities : Primary gangrene of the external genital organs (5% of cases), also called Fournier's disease, first described in 1883 : gangrene of the integument of the external genital organs in the absence of a local cause, according to an unknown mechanism and with inevitable progression towards a specific distribution of necrosis, regardless of treatment. "Secondary" gangrene of the external genital organs (95% of cases) related to a local, cutaneous, urogenital or gastrointestinal cause, whose course can be modified by early application of appropriate treatment. An early diagnosis is essential : in true Fournier's disease to avoid the systemic complications of this necrosis, and in secondary gangrene to limit the local extension and to prevent systemic complications. In view of the galloping course of the disease and in order to very rapidly reach the diagnosis, many authors recommend : local aspiration, biopsy or search for subcutaneous gas by ultrasound or radiology. Treatment consists of : Antibiotic therapy using two or three antibiotics to cover the usually mixed bacterial flora (Gram positive cocci, Gram negative bacilly and anaerobes). Surgical debridement as required, possibly repeated to excise necrotic zones, to drain collections and for irrigation of cavities. Hyperbaric oxygen therapy. Urinary diversion (cystostomy) or gastrointestinal diversion (colostomy) may be required when either of these two tracts are responsible for the infection, or when diversion facilitates management. Surgical reconstruction (grafts or flaps) as required. The mortality is high, of the order of 20% : < 10% in young patients with a good general condition who are correctly treated after an early diagnosis; > 50% in elderly patients with other concomitant diseases, when diagnosis and treatment are delayed.

Diagnosis, Differential↗
PubMed1995

[Intracaval neoplastic thrombi from clear cell adenocarcinoma of the kidney involving the righ renal and spermatic veins. Report of a case].

The authors report the case of a patient with right renal cell carcinoma with two intracaval tumour nodules, one arising in the renal vein and the other arising in the spermatic vein. They discuss the complementary investigations and propose a pathophysiological explanation for this rare neoplastic extension to the spermatic vein.

Adenocarcinoma, Clear Cell↗
PubMed1994

Identification of two nuclear N-acetylglucosamine-binding proteins.

Using neoglycoproteins, lectins that recognize different sugars, including N-acetylglucosamine residues, were previously detected in animal cell nuclei. We report herein the isolation of two N-acetylglucosamine-binding proteins from HL60 cell nuclei: i) a 22 kDa polypeptide (CBP22) with an isoelectric point of 4.5 was isolated for the first time and ii) a 70 kDa polypeptide with an isoelectric point of 7.8. This latter protein corresponds to the glucose-binding protein (CBP70) previously isolated, based on the following similarities: i) they have the same molecular mass, ii) they have the same isoelectric point, iii) they are recognized by antibodies raised against CBP70, and iv) both are lectins from the C group of Drickamer's classification. CBP70 appeared to recognize glucose and N-acetylglucosamine; however, its affinity for N-acetylglucosamine was found to be twice that for glucose. The presence in the nucleus of two nuclear N-acetylglucosamine-binding proteins and their potential ligands, such as O-N-acetylglucosamine glycoproteins, strongly argues for possible intranuclear glycoprotein-lectin interactions.

Acetylglucosamine↗
PubMed1994

Evidence that lactose binding to CBP35 disrupts its interaction with CBP70 in isolated HL60 cell nuclei.

We have previously reported that two carbohydrate-binding proteins (CBP35 and CBP70) can, under appropriate conditions of affinity chromatography, be isolated from HL60 cell nuclear extracts as a complex. Moreover, we have demonstrated that, during affinity chromatography, the CBP70-CBP35 association can be modified by the binding of lactose to CBP35. To determine whether the CBP70-CBP35 association could be disrupted in the nucleus upon lactose binding to CBP35, the behaviors of CBP70 and CBP35 were analyzed in membrane-depleted nuclei of HL60 cells, incubated with or without lactose. This study was performed by using an antiserum that cross-reacts with CBP35 and CBP70, an antiserum that was specifically raised against CBP70, and a monoclonal antibody (Mac 2) reactive against CBP35. Taken together, the results of indirect immunofluorescent staining, immunoblotting experiments, and quantitative flow-cytofluorometric analysis show that (i) CBP70 and CBP35 are present and colocalized in the nuclei incubated without lactose, (ii) all the CBP70 molecules left the nuclei incubated in the presence of lactose, while CBP35 molecules, probably bound to RNA, remained inside the nuclei, and (iii) glucose failed to have the same effect as lactose. These results strongly suggest that, in membrane-depleted nuclei, CBP35 and CBP70 interactions can be altered by a conformational change of CBP35 induced by the binding of lactose to its carbohydrate-recognition domain.

Antibodies, Monoclonal↗
PubMed1994

Renal transplantation in patients over sixty years of age.

The aim of this study was to analyze whether old age affects the outcome of renal transplantation. Data were presented on all 337 renal allografts performed from January 1, 1987 to November 30, 1992 in the department of Nephrology and Urology, University Hospital of Nancy. Of these, 32 (9.5%) were performed in patients over 60 years old at the time of transplantation (mean duration of follow-up 22.3 +/- 17.1 months). No significant difference was noted in patient and graft survivals between the two groups at 36 months (respectively 83.8% and 76.1% in elderly patients; 96% and 82.8% in younger recipients). In the older group, all grafts were lost due to death (2/5) or nephrectomy (3/5) with a functional transplant (3/5) whereas chronic rejection accounted for the majority of graft loss in the younger group (23/43, p < 0.05). Episodes of acute rejection occurred with a very low incidence in elderly patients (15.6%). Infections were infrequent in this group and did not represent serious complications. Functional rehabilitation and quality of life were as good in elderly as in younger recipients. These results suggest that renal transplantation is an acceptable form of treatment for patients older than 60 years with end-stage renal disease in the absence of obvious contraindication.

Adult↗
PubMed1994

[Value of three-dimensional surface reconstruction scanning in urology. Preliminary results].

The development of helicoidal CT acquisition allows 3D reconstruction of vessels, cavities or parenchyma of mobile organs such as the kidney. The value of this 3D surface reconstruction was investigated in various areas of urology: congenital malformations (horseshoe kidney, megaureter, ectopic organs), acquired malformations (diverticulum, trabeculated bladder), renal stones, particularly staghorn calculi, and renal transplantation. The fields of interest of 3D CT scans are still poorly defined, but are probably threefold: diagnostic, therapeutic and for teaching purposes. 3D representation completes classical axial scans and, in some cases, competes with other more invasive imaging techniques.

Female↗
PubMed1994

[Tubulo-papillary tumors of the kidney. Clinical, histologic and cytogenetic aspects. 15 new cases].

Between 1987 and 1992, we operated on 15 patients with tubulopapillary tumours (TPT) of the kidney, corresponding to 10% of all renal cancers operated during this 5-year period, which brings the total number of published cases to 418. TPT differ from non-tubulopapillary tumours in several ways. The medical imaging findings may differ when the tumour is not necrotic (20 to 30% of cases). The histology differs not only in terms of the tumour architecture, but also by the frequent association (9/15 cases) with other ipsilateral or contralateral renal anomalies: 2 bilateral tumours, 3 tumours associated with multiple cysts and 4 multifocal tumours. These characteristics, combined with the finding of 12 familial cases suggest a genetic origin for TPT, especially as the genetic abnormalities detected (tri-or tetrasomy 17) differ from the anomalies usually detected in non-tubulopapillary tumours (anomalies of 3p and 5q). This study is the first to report familial cases of TPT. The prognosis of these tumours, generally better than that of non-tubulopapillary tumours, suggests the possibility of conservative surgery when the diagnosis can be established preoperative or at operation.

Adenocarcinoma, Papillary↗