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Biomedical subjects

J Honda

Publications and source records attributed to J Honda.

90 records · Page 5Linked to original sources
PubMed1990

Effect of serum derived blocking factor (SDBF) on platelet associated-IgG.

We analyzed the effect of an unknown factor, which derived from serum, on platelet associated IgG (PA-IgG) and we established the novel immunofluorescence method to measure the level of PA-IgG. Blood samples were obtained from 20 patients with idiopathic thrombocytopenic purpura (ITP). After the treatment of platelets with neuraminidase, the value of PA-IgG positive platelets (%PA-IgG) were significantly increased in patients with ITP, but not in controls. When the platelets pretreated with neuraminidase were incubated with human serum, %PA-IgG was remarkably decreased in ITP patients. In healthy individuals, when platelets bound to aggregated human IgG pretreated with human serum, the binding of FITC-conjugated anti-human IgG was inhibited. And the value of mouse anti-GP II b/III a monoclonal antibody positive platelets was decreased, when the platelets preincubated with mouse serum. The binding of FITC-conjugated anti-human IgG and anti-mouse IgG antibody was apparently inhibited by some unknown factor in serum. Our results showed that this substance had species specificity. We called this substance "Serum Derived Blocking Factor (SDBF)".

Adolescent↗
PubMed1990

[Acute myelomonocytic leukemia with mastocytosis in bone marrow].

A 22-year-old female was admitted to our hospital because of general fatigue. The lymph nodes, liver and spleen were not palpable. She was without cutaneous lesions. Haematological examinations revealed leukocytes 3,200/microliters with 44% blasts of myelomonocytic origin, and platelets 15,000/microliters. Bone marrow smears were hypercellular marrow with 51% blasts of myelomonocytic origin and focal involvement of mast cells. Serum histamine and vitamin B12 level was high. Mast cells were round with rounded or segmented nuclei. The nucleoli were inconspicuous and the cytoplasm contained a number of metachromatic granules. Cytochemically, mast cells stained positive for alpha-naphthol-AS.D-chloroacetate esterase and acid phosphatase, and negative for peroxidase, Sudan black B and alpha-naphthyl butylate esterase. In toluidine blue staining, mast cells had stained similarly with pH values from 2.5 to 6.5. She was diagnosed as acute myelomonocytic leukemia with benign mastocytosis, and treated with BH.AC-DNP. A complete remission was obtained, but mast cells in the marrow did not decrease. Relationship between leukemia and mastocytosis was not known, but it was suggested that mast cells responded to the proliferation of the leukemic cells.

Adult↗
PubMed1989

[Remission of a non-haemophilic patient with acquired factor VIII inhibitor treated with infusion of factor VIII and corticosteroid].

A 61-years old woman who had been healthy without history of abnormal bleeding, developed widely spread ecchymosis and intramuscular bleeding in March, 1987. She was hospitalized for this hemorrhagic diathesis in May, 1987 and the following laboratory data were revealed: activated partial thromboplastin time (APTT), 76.7 seconds; factor VIII procoagulant activity, 2%; factor VIII inhibitor, 27 Bethesda units/ml. The inhibitor was an immunoglobulin of IgG type. Her clinical data of the blood were normal, and tests for antibodies, such as RA test, LE test and thyroid test were negative. Physical examination revealed ecchymosis over her right arm and swelling and pain in the right arm. She was first treated with a large dose of factor VIII concentrates, but the effect was insufficient. Then prednisolone was given, which resulted in decreasing of the inhibitor and improvement of the coagulation profiles. This treatment appeared to offer effective control on severe hemorrhage in patients with factor VIII inhibitors.

Autoantibodies↗
PubMed1987

Functional heterogeneity of human monocytes--with a special reference to flow cytometric assays.

A large number of human mononuclear cells were simultaneously separated into fractions enriched in B cells, T cells, large granular lymphocytes (LGL) and monocytes by centrifugal elutriation. Lymphocyte populations were analyzed using monoclonal antibodies. In particular, highly enriched natural killer cells, Leu7+ cells, were collected in the intermediate fractions. Monocytes, which were identified as esterase positive cells, and Leu M3 cells were collected at higher counterflow rates and in the final fraction. The purity of monocytes in the final fraction was 81%. The oxidative metabolic activity (H2O2 production) and non-specific esterase activity of individual monocytes was estimated in the analysis of functional heterogeneity of monocytes using flow cytometry. 2',7'-dichlorofluorescein diacetate (DCFH-DA) and fluorescein diacetate (FDA) were used as indicators in the measurement of H2O2 generation and esterase activity. Intracellular generation of a fluorescence product (H2O2 Production; average percentage of fluorescence positive cells) of monocytes in the stimulation of phorbol myristate acetate (PMA, 100 ng/ml) was greater in larger than smaller cells. H2O2 production gradually increased from 6% and 25-38% and 60% in the intermediate and final fractions respectively. Furthermore, the average fluorescence intensity of the large monocyte population in the final fraction was 1.13-1.31 fold more active than that of the smaller cells. Thus, the functional heterogeneity of human monocytes was further confirmed in the assays of H2O2 production exposed to PMA and FDA hydrolysis using flow cytometry. Furthermore, the CCE system can isolate lymphocyte subsets and LGL.

Antigens, Surface↗
PubMed1982

[Pathogenetic study of complete and partial hydatidiform mole by HLA (author's transl)].

Twenty four complete and 15 partial moles, and the lymphocytes from the parents were analyzed for human lymphocyte antigen (HLA-A and HLA-B specificities), and karyotyping was done in each molar tissue by the direct method. Twelve of the 24 complete moles expressed homozygous A and/or B specificities identical to those of the father. No cases expressed A or B specificities derived from the maternal side. All had a 46, XX karyotype. On the other hand, in 6 of the 15 partial moles, the antigen specificities were heterozygous for A locus and 10 of the 15 expressed A and/or B specificities derived selectively from the maternal side. In chromosome studies, 8 of the 15 had a 69,XX karyotype, 5 had a 46,XX karyotype and 2 was impossible of analysis. These results show that complete moles are mostly androgenetic in origin but partial moles are not; that is, pathogenesis distinguishes complete from partial moles.

Adult↗
PubMed1982

[Incidence of hydatidiform mole and aging of mothers (author's transl)].

The incidence of hydatidiform mole, found in Fukushima Prefecture from 1975 to 1979 (5 years), in all pregnant women was statistically studied according to their age. 1) The number of pregnant women was 238,776, and the number of hydatidiform moles was 478. The incidence of hydatidiform mole in pregnant women was 2.00%. 2) The incidence of hydatidiform mole was approximately 1-2% in mothers under 40. The incidence tended to be greater in mothers over 40. The incidence was 27.26% in mothers aged 45 to 49 and 303.03% in mothers over 50 (150 times greater than usual). These results show a clear relationship between the incidence of hydatidiform mole and the mothers age. We believe an increase in abnormal ova is the cause of the increased incidence of hydatidiform mole in older mothers.

Adult↗
PubMedDate not supplied

Effect of heat-pretreatment on interleukin-2-activated killer cells for in vitro purging.

OBJECTIVE: Lymphokine-activated killer activity is effective in purging residual malignant cells from autologous bone marrow (BM) or leukapheresis collections for peripheral blood progenitor cells (PBPC). In this study, the effect of additional heat pretreatment on IL-2-activated cytotoxicity was examined. METHODS: Nonadherent mononuclear cells from peripheral blood (PB), PBPC collections or BM were exposed to the desired temperature for 1 h, and successively cultured for 7 days in the presence of 1,000 units/ml of recombinant human interleukin-2 (IL-2). Cytotoxicity of IL-2-activated cells was determined by flow cytometry, using PKH-26-labelled K-562 or Raji target cells, and the cell surface antigens were also analyzed. RESULTS: IL-2-activated cells from all specimens showed a distinct generation of cytotoxic activities. The activities were significantly decreased by heat at 42 degrees C, but not changed by heat pretreatment at 40 degrees C. In the surface expression of IL-2-activated cells, the increase of CD56+ cells from PB, PBPC collections and the decrease of CD28 cells from PBPC collections were shown to be significant (p < 0.05), but these changes were not found when the cells were heat-pretreated at 42 degrees C. DISCUSSION: These results suggested that a tumor-killing therapeutic temperature (> or =42 degrees C) may lead to a disadvantage in posttransplantation immunity, and that this reduction of IL-2-activated cytotoxicity may partly be due to a lack of the enhancement of CD56+ cells or the reduction of CD28+ cells.

Antigens, CD↗