Search PubMed⌕ Search

Biomedical subjects

J Holm

Publications and source records attributed to J Holm.

At least 235 records · Page 13Linked to original sources
PubMed1982

Traumatic intimal tear of the renal artery without thrombosis.

After a skiing accident a 15-year-old boy contracted a 1 cm long intimal tear of the distal part of the main renal artery on the left side demonstrated angiographically. Radiorenography showed a curve compatible with partial obstruction from the injured kidney while the other side was normal. The operation was delayed for 5 days and renewed preoperative angiography showed the same picture as 4 days previously. At operation it was found that an approximately 1 cm long segment of the intima was completely detached from the arterial wall. The loose fragments were removed and the distal intimal ends secured to the vascular wall. The longitudinal arteriotomy was closed directly without vein patch. The postoperative course was uneventful and repeated post-operative radiorenograms have shown normal registrations from the left kidney. This case illustrates that an intimal tear may stay open for 5 days and that it can be successfully treated by simple removal of the loose fragments.

Abdominal Injuries↗
PubMed1982

Maximum folate binding in leukocyte lysates and sera from blood donors.

Maximum binding of [3H] folate was determined in leukocyte lysates and sera from blood donors with normal hematological parameters. The folate binding activity in leukocyte lysates showed a remarkably great interindividual variation, whereas the folate binding in sera from the same persons showed a rather modest interindividual variation. The folate binding activity in leukocyte lysates is mainly derived from granulocytes since lymphocytes and monocytes from individuals with high content of folate binder in their leukocytes contained less than 1% of the total binding activity.

Adult↗
PubMed1982

Gel filtration profile of folate binding proteins in Triton X-100 solubilized human raw milk.

Solubilization of human raw milk with Triton X-100 gave rise to a two-fold increase in maximum [3H] folate binding as determined in equilibrium dialysis experiments (pH 7.4, 37 degrees C). Gel filtration studies on Triton X-100 solubilized human milk revealed three distinct peaks of protein-bound [3H] folate, Mr approximately 25 000 (38%), Mr greater than or equal to 130 000 (15%) and Mr approximately 100 000 (47%). The first peak was identical to the folate binder previously isolated from human whey, while the second peak most likely represented a particulate membrane-bound form of that binder. The third so far undescribed peak (Mr greater than or equal to 100 000) seemed to contain hydrophobic residues, and might be encapsulated in Triton X-100 micelles. This peak may represent a Triton X-100 solubilized split product of the large particulate binder.

Carrier Proteins↗
PubMed1982

Pyogenic liver abscess: a study of forty-four cases in two centres.

44 patients with pyogenic liver abscess, 22 each from Leicester, UK, and Göteborg, Sweden, presenting between 1972 and 1980 have been reviewed. 29 patients were diagnosed prior to specific treatment with 3 deaths (10%); 15 patients were first discovered at autopsy resulting in an overall mortality of 41%. The presentation, pathogenesis, and treatment are discussed in detail. It is concluded that the continuing high mortality of this condition largely relates to the difficulty in establishing the diagnosis. Reduced morbidity (and perhaps mortality) might be achieved with wider use of percutaneous methods of drainage.

Adolescent↗
PubMed1981

Energy metabolism in relation to oxygen partial pressure in human skeletal muscle during exercise.

1. The intramuscular oxygen partial pressure (pO2) in human gastrocnemius muscle was monitored during exercise and compared with metabolite concentrations reflecting the energy and the redox state in the tissue. Ten normal subjects and ten patients with peripheral vascular occlusive disease were investigated. 2. In normal subjects the pO2 at the end of exercise was related to the intensity of the exercise, expressed as effect (J/s) per contraction. 3. In both patients and normal subject the pO2 was related to the [ATP]/[ADP] ratio, the [lactate/[pyruvate] ratio and the phosphocreatine concentration in the muscle tissue at rest and during exercise. 4. At each pO2 value, a lower [lactate/[pyruvate] ratio was found in the muscle tissue of the patients compared with that of normal subjects. This was interpreted as a beneficial effect of the higher oxidative-enzyme capacity in the muscle of the patients. 5. The results show the importance of pO2 for the regulation of the energy and the redox state of the tissue. During exercise the changes induced in pO2 and thus the energy state will stimulate the respiratory rate. This might be an important link in triggering the oxidative-enzyme capacity in response to physical training as well as in peripheral vascular occlusive disease.

Energy Metabolism↗
PubMed1981

Response to busulphan treatment of paroxysmal nocturnal hemoglobinuria and myelofibrosis in one and the same patient.

Paroxysmal nocturnal hemoglobinuria (PNH) occurs occasionally in association with other blood disorders. This fact has led to the suspicion, that a mutation on the stem cell level may produce a dual defect of hematopoiesis, the PNH defect as well as other disturbances in blood formation. A patient with PNH and myelofibrosis appearing simultaneously is presented. Treatment with busulphan resulted in reduction of the hemolysis and improvement of the patient's condition. To our knowledge, this experience has not been described previously.

Blood Cell Count↗
PubMed1981

High-affinity folate finding in chronic myelogenous leukaemia serum, relationship of binding characteristics to activity of disease and binder concentration.

High-affinity binding of [3H]folate to chronic myelogenous leukaemia serum displayed positive co-operativity. There seemed to be a relationship between the concentration level of binder in serum and the activity of the disease in the sense that the serum level of binder during remission fell to a value similar to that of normal serum. This was accompanied by a shift from positive co-operativity to the binding type of normal serum, i.e. negative co-operativity. The data support the hypothesis that the different characteristics of high-affinity folate binding in chronic myelogenous leukaemia and normal serum are related to parallel differences in the serum level of binder.

Carrier Proteins↗
PubMed1981

High-affinity binding of folate to a serum protein in chronic myelogenous leukemia: effect of binder concentration, pH, and temperature.

Serum from a patient with chronic myelogenous leukemia was subjected to anion-exchange chromatography on DEAE-Sepharose CL-6B. High-affinity binding of [3H]folate to front effluent, eluted at a low salt gradient, was studied in equilibrium-dialysis experiments (37 degrees C, pH 7.4). As suggested by the data, folate binding displayed positive cooperativity. Dilution of the binder solution resulted in a shift to a simple non-cooperative binding type and increased binding affinity. Furthermore, binding was inhibited at pH 5.0 and at low temperature (7 degrees C). This study demonstrates important similarities between high-affinity folate binding in milk and serum: positive cooperativity and dependence of binding affinity on concentration of binder. Identical mechanisms may underly these phenomena in milk and serum. The apparent relationship between binding type and concentration of binder shown herein seems to agree fairly well with recent observations on sera from groups of healthy persons.

Carrier Proteins↗
PubMed1981

Evidence that the low-affinity folate-binding protein in erythrocyte hemolysate is identical to hemoglobin.

Gel filtration studies on erythrocyte hemolysate demonstrated the presence of a folate binding protein, apparently of the low-affinity type, that co-elutes with hemoglobin. Further, the folate binder eluted with a low salt concentration after DEAE-Sepharose CL-6B anion-exchange chromatography of erythrocyte hemolysate at pH 6.3. The chromatographic behavior of hemoglobin labeled with [3H]folate was so similar to that of the present binder as to suggest that the folate binder in erythrocytes is in fact hemoglobin.

Carrier Proteins↗
PubMed1981

Sources and routes in postoperative wound infections.

In a prospective study including 112 patients sources and routes in postoperative wound infections were traced by comparing wound cultures with cultures from the patient, other patients, personnel and from the environment in the operation unit, intensive care unit and in the ward. A total number of about 10 000 cultures was taken. The wound infection rate was 9.8% (8.1% in clean surgery and 13.2% in potentially contaminated surgery). The patient was a source of the bacteria in all cases of wound infection after potentially contaminated surgery and the wounds were probably contaminated intraoperatively. A possible intraoperative wound inoculation was registered at four instances and a postoperative wound inoculation at four instances in clean surgery. It is concluded that surgical wounds can be contaminated both at surgery and postoperatively. Postoperative wound contamination seems to be most important in clean surgery.

Adult↗
PubMed1980

High and low affinity binding of folate to proteins in serum of pregnancy women.

Binding of [3H]folate to proteins in serum of pregnant women was studied in equilibrium dialysis experiments (pH 7.4, 37 degrees C). A Scatchard analysis revealed the presence of high-affinity (Kass = 10(10) M(-1), N = 0.4 nM folate) and low-affinity sites. The high-affinity folate binding protein (Mr approximately 30 000--35 000) appeared in front effluent after application of serum to a DEAE-Sepharose CL-6B column equilibrated with 0.05 M imidazole buffer (pH 6.3)/30 mM NaCl. Low-affinity binding protein eluted from the column after a rise in NaCl concentration to 1 M was mainly similar to albumin. A minor part was, however, associated with a large molecular size (Mr > 200 000) protein, probably alpha 2-macroglobulin. High-affinity binding which displayed positive cooperativity was saturated at folate concentrations above 10(-10) M. Folate dissociation was a complex process consisting of an initial rapid phase (terminated within 48 h) followed by a slow release. At pH 3.5 dissociation became rapid and complete. Purified methotrexate had no effect on high-affinity binding, whereas N10-methylfolate (an impurity in the methotrexate preparation) acted as a potent inhibitor. Low-affinity binding was proportional to the folate concentration within the range 10(-10)--10(-7) M. Dissociation of folate was rapid.

Binding Sites↗
PubMed1980

High-affinity binding of folate to a protein in serum of male subjects.

The binding pattern of [3H]folate in the serum of male subjects was studied in equilibrium dialysis experiments (pH 7.4, 37 degrees C). It was possible to distinguish between a high (association constant, Kass = 10(10) (mol/l)-1; maximum binding of folate = 0.6 nmol/l) and a low affinity type of binding. The high-affinity binder was a trace protein (molecular size approximately 35 000) which was eluted with 30 mmol/l NaC, (pH 6.3) from serum subjected to DEAE-Sepharose CL-6B chromatography. The low-affinity binding activity mainly associated with albumin was eluted with 1 mmol/l NaCl. High-affinity binding was depressed at 7 degrees C (Kass = 10(9) (mol/l)-1). Furthermore, methotrexate acted as a weak inhibitor of high-affinity binding, the molar methotrexate/folate ratio being 100/1 at 50% inhibition.

Chromatography, Ion Exchange↗