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Biomedical subjects

J Holm

Publications and source records attributed to J Holm.

At least 199 records · Page 11Linked to original sources
PubMed1985

T-cell subsets in multiple myeloma. Impact of cytostatic treatment.

Blood leukocyte and lymphocyte counts, absolute and relative numbers of T-lymphocytes and T-cell subsets were studied in 45 patients with multiple myeloma and 18 healthy controls. No differences were found between untreated patients and controls. In the group of untreated patients similar results were obtained in patients with low-intermediate tumour cell mass as in patients with large tumour cell mass. In patients with large tumour cell mass studied during cytostatic therapy, leukocyte and lymphocyte counts, T-cells, OKT4+ cells and OKT4/OKT8 ratios were significantly lower than in untreated patients. Patients studied at varying intervals (2-28 mo.) after cessation of therapy still exhibited abnormal leukocyte and lymphocyte counts, numbers of T-cells, OKT4+ cells and OKT4/OKT8 ratios.

Aged↗
PubMed1985

A high-affinity folate binding protein in normal human leukocytes: ligand binding characteristics, ionic charge and molecular size.

High-affinity binding of [3H]folate to supernatant from homogenized human leukocytes containing large amounts of binding protein displayed apparent positive cooperativity. The DEAE-Sepharose CL-6B chromatographic profile of the supernatant at pH 6.3 contained a major peak of folate binding (Mr approx. 25 000) in the front effluent and a smaller more acidic peak (Mr approx. 25 000) that emerged after a rise in NaCl from 30 mmol/l to 1 mol/l. Triton X-100 solubilized cell sediment from the leukocyte homogenate contained some high-affinity folate binding activity (Mr approx 25 000), typically 5-10% of the total binding activity.

Carrier Proteins↗
PubMed1985

Epidural electrical stimulation in severe limb ischemia. Pain relief, increased blood flow, and a possible limb-saving effect.

Peripheral vascular disease of the extremities causes ischemic pain and, at times, skin ulcerations and gangrene. It has been suggested that epidural spinal electrical stimulation (ESES) could improve peripheral circulation. Since 1978 we have used ESES in 34 patients with severe limb ischemia; all had resting pain and most had ischemic ulcers. Arterial surgery was technically impossible. Twenty-six patients had arteriosclerotic disease, one had Buerger's disease, and seven had severe vasospastic disorders. Ninety-four per cent of the patients experienced pain relief. ESES healed ulcers in 50% of those with preoperative nonhealing skin ulcerations. Seventy per cent of the patients showed improved skin temperature recordings. Only 38% of the stimulated arteriosclerotic patients underwent amputations during a mean followup period of 16 months, as compared to 90% of a comparable group of unstimulated patients. ESES is very promising in severe limb ischemia where reconstructive surgery is impossible or has failed.

Adult↗
PubMed1985

Staging of idiopathic myelofibrosis. Significance of haemoglobin value and reticulocyte count.

A retrospective analysis of 18 patients with idiopathic myelofibrosis (IMF) suggested that IMF is adequately staged by combining haemoglobin value and reticulocyte count. Four stages were recognized. All deaths occurred in late stage. The stages appeared to reflect a sequential course of the disease. The present staging system adequately identified patients at high risk. However, the rate of disease progress varied greatly. It is therefore suggested that the rate of disease progress should also be considered when an individual patient is evaluated.

Aged↗
PubMed1985

A high-affinity folate binding protein in human cerebrospinal fluid.

Binding of 3H-folic acid to a protein in human cerebrospinal fluid (CSF) was studied in equilibrium dialysis experiments at 37 degrees C and pH 7.4. The binding was of a high-affinity type (10(10) l/mol), and the relative molecular mass of the protein was 25,000 as determined by gel filtration. The concentration of folate binding protein in CSF, expressed as maximum folate bound per 1, was 0.3 nmol/l. This value was of the same order of magnitude as that of serum.

Carrier Proteins↗
PubMed1985

Li+-induced release of the folate binding protein from human granulocytes.

Release of folate binding protein from human leucocytes suspended in isotonic NaCl was studied in experiments where folate binding activity of the suspending medium was determined by equilibrium dialysis (pH 7.4, 37 degrees C) against 3H-folate. Release from leucocytes containing particularly high concentrations of folate binding protein was significantly increased in the presence of 70 mmol/l Li+. The stimulated release was apparently not an osmotic phenomenon since no effect was observed with isomolar Na+/K+. The released protein resembled the folate binding protein in leucocyte lysate as to characteristics of radioligand binding, ionic properties and molecular size. Hence no transformation of the protein seemed to occur during Li+-induced release.

Carrier Proteins↗
PubMed1985

Expression of class II transplantation antigen on vascular smooth muscle cells in human atherosclerosis.

A large proportion of the cells of the human atherosclerotic plaque is assumed to be derived from medial smooth muscle cells. In contrast to these, the cells of the plaque have the capacity to accumulate lipid, and they also proliferate at a higher rate than medial cells. It has therefore been suggested that smooth muscle cells undergo a change of phenotype during atherogenesis, but there has been no evidence for such a change on the molecular level. We have now analyzed carotid artery plaques using a battery of antibodies against cell surface and cytoskeletal antigens, and found that most of the cells express the class II transplantation antigen (Ia antigen) HLA-DR. Also, the beta chain of HLA-DR was detected by immunoblotting of plaque extracts with the OKIa1 monoclonal antibody. HLA-DR is normally present on cells of the immune system, but only 60% of the DR-positive cells of the plaque reacted with monoclonal antibodies specific for macrophages and lymphocytes. Many of the remaining DR-positive cells contained the muscle-specific intermediate filament protein, desmin. This indicates that smooth muscle cells of atherosclerotic plaques express DR antigen. In contrast, very few DR-positive cells were found in normal human arteries. This suggests that expression of class II antigen is part of a phenotypic change in smooth muscle cells in atherosclerosis.

Aged↗
PubMed1985

Lower-extremity ischemia in aortic dissection. Report of three cases.

Reports are presented of three hypertensive men with aortic dissection and signs of peripheral (leg) ischemia. The dissection was of type III b in two cases and type I in one case. Hypotensive medication was given in all cases, but in two of them peripheral vascular surgery became necessary to re-establish the circulation, while in the third case the hypotensive treatment sufficed for improvement of the leg circulation. Collaboration between thoracic and vascular surgeons can be of value in cases of aortic dissection with peripheral ischemia.

Aged↗
PubMed1985

Charge heterogeneity of the folate binding protein in normal human leukocytes.

The DEAE-Sepharose CL-6B chromatographic profile of supernatant from homogenized normal human leukocytes containing large amounts of folate binder revealed two peaks of binding activity. A minor binder (I) eluted with the equilibrating buffer (1 mM sodium phosphate of pH 6.0), while the major binder (II) first eluted after the initiation of a linear phosphate gradient with 200 mM sodium phosphate of pH 7.6 as the limiting buffer. Binder II was thus a more acidic protein since it required elution by a salt-pH gradient. Binding of [3H] folate to binder II was of a high-affinity type (K = 10(10) M-1) and displayed positive cooperativity.

Carrier Proteins↗
PubMed1984

Treatment of advanced bone marrow neoplasms with ifosfamide combinations.

Ifosfamide, in combination with other cytostatics, was given to 15 patients with advanced neoplasia derived from bone marrow cells. A response to therapy was obtained in 8 patients. It is suggested that ifosfamide, in combination with other drugs, deserves larger trials in the treatment of advanced bone marrow neoplasia.

Adolescent↗
PubMed1984

Accumulation of IgG and complement factor C3 in human arterial endothelium and atherosclerotic lesions.

Endothelial injury is associated with an intracellular accumulation of IgG. Fluorescent antibodies were used to detect IgG and complement factor C3 in biopsies of human arteries. Both proteins were found in endothelial cells with signs of injury, in normal and atherosclerotic arteries. Rhodamine-conjugated anti-IgG and FITC-conjugated anti-C3 were used to count the number of IgG- and C3-containing endothelial cells in non-atherosclerotic cystic arteries. IgG was found in 26% of the cells, with C3 in 38% of the IgG-containing cells. Both IgG and C3 were also observed in the subendothelial intima of cystic arteries and in atherosclerotic lesions of iliac and femoral arteries. The accumulation of IgG and C3 in injured endothelium and in atherosclerotic lesions could be of importance for complement-mediated tissue destruction and for the attraction and activation of white blood cells.

Adult↗
PubMed1984

3-Hydroxy-3-methylglutaric aciduria.

3-Hydroxy-3-methylglutaric aciduria was found in a newborn infant whose parents are first cousins. The patient presented at 5 days of life with hyperammonemia, hypoglycemia, and metabolic acidosis. There was no ketonuria. Diagnosis was made by analysis of the pattern of organic acids excreted in the urine. A profound deficiency in activity of 3-hydroxy-3-methylglutaryl-coenzyme A lyase was found in cultured skin fibroblasts. The parents had intermediate levels of enzyme activity.

Cells, Cultured↗