Structure and function of phage RNA.
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Biomedical subjects
Publications and source records attributed to J Hindley.
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The sequence of the first 52 nucleotides at the 5'-end of the Qbeta minus strand has been determined and found to be complementary in an antiparallel fashion to the 3'-terminal region of the Qbeta plus strand. There are few similarities between the corresponding sequences of Qbeta plus and minus strands; however, both have a hydrogen-bonded loop close to the 5'-end. The sequence of the 3'-terminal region of the plus strand was deduced: there are no termination signals within the last 32 nucleotides. A notable homology exists between the 3'-ends of Qbeta and R17 RNA.
A new approach for the localization of a cistron on a phage RNA has been developed. The coat protein cistron of phage Qbeta was found to begin between the 1100th and 1400th nucleotide from the 5' terminus of Qbeta RNA and therefore lies in the middle of the genome.
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