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Biomedical subjects

J Hill

Publications and source records attributed to J Hill.

At least 217 records · Page 12Linked to original sources
PubMed1994

Scintigraphic evaluation of sialadenitis.

Salivary gland scintigraphy using 99Tcm sodium pertechnetate and a 20-min dynamic data acquisition with a sialogogue at 10 min was employed as the primary imaging modality in sialadenitis in 32 patients. Paired asymptomatic glands within the study group were used to establish control values for a number of computer-derived functional parameters. The shape of the activity-time curve was described as: H = hyperactive, N = normal, M = median, S = sloped, F = flat, O = obstructed. Of seven patients who had ductal stones on plain films, two passed stones prior to sialography and had normal curves, whole the remaining five patients had Type O curves. Parametric evidence of gland dysfunction was identified in an additional seven symptomatic glands, six being hyperactive; histology identified an acute inflammatory infiltrate in the only excised gland in this group. Glandular hypofunction was identified in one case, in which histology revealed extensive fibrosis and glandular atrophy. Type M and S curves were found in 28 glands, 75% of which were asymptomatic. Only one gland was indicated as hypoactive by calculated parameters, and this showed a Type S curve. Scintigraphy and plain films represent the least invasive and, we feel, the most suitable primary method of investigating sialadenitis. Conventional contrast sialography may be reserved for cases with no identifiable stone but an obstructed curve. The use of functional parameters and curve morphology to determine gland dysfunction other than obstruction is non-specific, probably insensitive and is unlikely to alter clinical management. We propose an algorithm for the investigation of sialadenitis.

Adolescent↗
PubMed1994

Should we routinely screen for Lyme disease in patients with asymmetrical hearing loss?

Asymmetrical sensorineural hearing loss is usually investigated thoroughly but a diagnosis is often elusive. It is important to identify any treatable causes. Lyme disease is a tick-borne infection caused by the spirochaete Borrelia burgdorferi, which has been shown to cause asymmetrical sensorineural hearing loss, and has been successfully treated. Its incidence varies regionally; it occurs where there is a suitable environment for the tick and its animal hosts. We conducted a prospective study of 100 patients with asymmetrical sensorineural hearing loss. Serological testing for Lyme disease was performed. One patient had positive serology, but there was no improvement in her hearing following antibiotic treatment. We cannot conclude that our patient's hearing loss was caused by Lyme disease. We suggest that routine screening is not cost effective in regions with a low incidence of Lyme disease.

Adolescent↗
PubMed1994

The effects of casting on upper extremity motor disorders after brain injury.

OBJECTIVES: This study compares casting to traditional techniques, such as passive range of motion, static stretch, and splinting, in the treatment of the hypertonic upper extremity in individuals with severe brain injury. METHOD: Fifteen subjects with brain injury were randomly assigned to one of two groups. One group received a month of casting followed by a month of traditional therapy; the second group received 1 month of traditional therapy followed by casting. The subjects' limbs were evaluated for range of motion, clinical indications of spasticity, and functional use of the extremity at three intervals--before intervention, after the first month of intervention, and after the second month of intervention. Two sample t-tests and paired t-tests were used in data analysis. RESULTS: All but one subject showed a greater improvement in range of motion with casting than with traditional treatment; 11 subjects showed a greater improvement in clinical measures of spasticity with casting. There was no apparent correlation between these measures and functional use of the extremity. CONCLUSION: These findings suggest that casting is more effective than traditional techniques in reducing contracture and in decreasing hypertonicity in some cases. The greater improvements in these motor indicators with casting did not translate into greater gains in functional use of the upper extremities.

Adolescent↗
PubMed1993

Human cathepsin E produced in E. coli.

A cDNA for procathepsin E was generated from human gastric adenocarcinoma (AGS) cells, amplified by PCR and inserted into the T7 dependent vector pET 22b for expression in E. coli. Purification of the resultant product was accomplished simply, without the need to resort to column chromatography. The recombinant protein displayed comparable properties to those of its naturally occurring counterpart. The yield of homogeneous active enzyme obtained was approximately 3 mg per 40 g of cells. This is sufficient to permit crystallisation and structural analysis to begin and a mutagenesis programme to examine structure/activity relationships now to be undertaken.

Adenocarcinoma↗
PubMed1993

Insight into the active-site structure and function of cytochrome oxidase by analysis of site-directed mutants of bacterial cytochrome aa3 and cytochrome bo.

Cytochrome aa3 of Rhodobacter sphaeroides and cytochrome bo of E. coli are useful models of the more complex cytochrome c oxidase of eukaryotes, as demonstrated by the genetic, spectroscopic, and functional studies reviewed here. A summary of site-directed mutants of conserved residues in these two enzymes is presented and discussed in terms of a current model of the structure of the metal centers and evidence for regions of the protein likely to be involved in proton transfer. The model of ligation of the heme a3 (or o)-CuB center, in which both hemes are bound to helix X of subunit I, has important implications for the pathways and control of electron transfer.

Amino Acid Sequence↗
PubMed1993

Restrained occupants on the nonstruck side in lateral collisions.

Of injury-producing collisions with high seat belt use, some 25% to 30% are lateral collisions. This paper describes some of the characteristics of those collisions as they relate to the front-seat occupant sitting on the side opposite to the impact. The data came from a stratified sample of in-depth crash investigations conducted in the Birmingham region in the period 1983 to 1989 involving current model cars. Crash severity was assessed using the Vehicle Deformation Index (VDI) of the Collision Deformation Classification (CDC) ratings and velocity change. Injury severity was assessed using AIS 85 for each body region. 193 cases of restrained occupants in nonstruck side collisions were examined. Of those occupants with head injuries of AIS < or = 2, 35% came out of the shoulder section of the seat belt. Of abdominal injuries of AIS < or = 2, 72% came from the seat belt itself. Interaction between front seat occupants was not a frequent cause of injury to the nonstruck side occupant. Some aspects of seat belt geometry might be changed so that the trajectory and loading of the nonstruck side occupant are improved.

Accidents, Traffic↗
PubMed1993

A study of haemostasis following tonsillectomy comparing ligatures with diathermy.

The use of diathermy to achieve haemostasis after tonsillectomy remains controversial. We have reviewed the English language literature, and found no convicting evidence that diathermy is any more likely to cause post-operative haemorrhage than the use of ligatures. The results of a prospective, randomized study of 1036 consecutive tonsillectomies are presented. No significant difference was found in post-operative haemorrhage rates when either diathermy or ligatures were used. Diathermy was found to reduce operating time compared to ligatures. The possibilities for day-case tonsillectomy are discussed.

Adolescent↗
PubMed1993

Pressure exerted by head bandages used in otological surgery.

The pressure exerted by head bandages at the operation site following ear surgery was measured using a balloon catheter and pressure transducer. The initial pressures achieved, their diminution with time and the relationship of pressure to the induction of headache in the patient were studied. The principal findings were that the standard otological head bandage is rarely tight enough to prevent haematoma formation, bandages have lost their efficacy after the first hour and headaches are associated with a significantly higher initial bandage pressure.

Bandages↗
PubMed1993

Antithyroid and goitrogenic effects of coal-water extracts from iodine-sufficient goiter areas.

Goiter in iodine-sufficient areas has been linked to water-borne goitrogens in watersheds and aquifers rich in coal and shale. In the present study, the potential antithyroid and goitrogenic effects of coal-water extracts (CWE) were investigated in vivo in rats after chronic and acute oral administration of CWE, and in vitro by a thyroid peroxidase (TPO) enzyme system. CWE was prepared by continuous extraction of ground (40 mesh) Appalachian coal with goitrogen-free water (GFW). Female Buffalo rats fed on Purina iodine-rich diet (12 micrograms I-/day/rat), were given ad lib CWE (50 mg/ml; approximately 20 mL/day/rat) or GFW (controls) for 2 months. At the end of the experiment, 125I 1 microCi, was injected i.p. and 4 h later the thyroid glands were removed, weighed, and analyzed histologically and for total 125I and 125I-labeled compounds. Rats on CWE had larger thyroid glands [7.2 +/- 0.3 mg/100 g (mean +/- SE) vs 5.0 +/- 0.5 controls; p < 0.005] with distinct histological changes of smaller thyroid follicles, some with columnar epithelium, and with more dense colloid than in controls, and had significant inhibition of the coupling mechanism for production of thyroid hormones [125MIT + DIT/125T3 + T4: 5.1 +/- 0.2 vs 3.9 +/- 0.1 controls, p < 0.005; and 125T3 + T4 (%): 10.6 +/- 0.3 vs 12.6 +/- 0.4 controls, p < 0.005]. Female Sprague-Dawley rats under the same conditions as Buffalo rats were given acutely by GI tube 2 mL of CWE (5 g/mL) or GFW (controls).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

The relationship of haemoglobin to serum erythropoietin concentrations in the anaemia of rheumatoid arthritis: the effect of oral prednisolone.

Previous studies of the erythropoietin response to anaemia in RA have yielded conflicting findings. Some have found the response to be impaired and others have found a normal response. We have compared erythropoietin (EPO) levels measured by radioimmunoassay, in 54 anaemic rheumatoid patients and 55 patients with iron deficiency anaemia but no inflammatory disease. The erythropoietin response in the rheumatoid patients was impaired compared with the control group (P < 0.025) but only seven rheumatoid patients showed a response which fell below the 95% confidence intervals predicted for the control group. Rheumatoid patients who fell within the highest quartile for serum ferritin concentrations (i.e. those most likely to have anaemia of chronic disease) had significantly lower EPO levels compared with the control group (P < 0.01). EPO levels in rheumatoid patients within the lowest quartile for ferritin (i.e. those with iron deficiency anaemia) were not significantly different from the control group (P = 0.670). The difference in EPO response between the RA patients in the upper and lower quartile for ferritin approached but did not achieve significance (P = 0.056). In a second study 15 anaemic RA patients were given a 5-day course of oral prednisolone 1.5 mgkg-1. Hemoglobin did not rise significantly until day 4 but EPO levels fell by day 1 (P < 0.005) and remained lower than pretreatment values throughout the study. Thus, in RA patients, anaemia of chronic disease is associated with inappropriately low EPO concentrations but this does not appear to be the major cause of the anaemia and Hb response to prednisolone does not depend upon an increase in EPO concentration.

Administration, Oral↗
PubMed1993

Optimizing the assessment of disease activity during treatment with anti-rheumatoid drugs.

Clinical trials in RA usually involve the use of several laboratory assessments of disease activity. Their use is not universal and the relative value of many novel assessments has not been determined in relation to existing clinical and laboratory methods. This study attempts to investigate the value of established and novel assessments of disease activity during treatment with accepted DMARDs. Over a 48-week study period, changes in cytidine deaminase (CD), beta 2-microglobulin, alpha 1-acid glycoprotein (alpha 1-AGP), serum antibodies to Clostridium perfringens alpha-toxin, pre-albumin and caeruloplasmin were compared to a group of established clinical and laboratory assessments including plasma viscosity, CRP haemoglobin and platelet count during treatment with the established second-line drugs, D-penicillamine (n = 20), sulphasalazine (n = 17), gold (n = 12) and hydroxychloroquine (n = 18). Overall, the assessments showing the greatest degree of change were plasma viscosity, articular index, summated change score, platelet count, CD, white cell count, alpha 1-AGP, CRP and pain score. The assessments showing the greatest degree of change were not homologous between the treatment groups and no single assessment was outstanding for a particular drug treatment.

Anti-Inflammatory Agents↗
PubMed1993

Mean airflow rates in laryngeal polyposis.

This study assesses the laryngeal airflow characteristics in 10 patients with laryngeal polyposis before and after surgical excision. The results indicate that mean airflow rates in the presence of polyps are a reflection of two mechanical processes. Narrow based polyps splint the glottis open during phonation, thus increasing flow rates, whereas large broader based polyps tend to obstruct the glottis and reduce the airflow. The results suggest that mean airflow rates may be used to measure the improvement in laryngeal function following surgical intervention in individual patients.

Adult↗
PubMed1993

Intraduct papilloma of parotid gland.

Intraduct papillomas of the salivary glands are rare solitary tumours. Most of the reported cases have occurred in minor salivary glands. A case which occurred in the parotid gland is reported. Fine needle aspiration was performed on this tumour, and the cytological appearances of the aspirate were suggestive of an adenoid cystic carcinoma. This is an important potential diagnostic pitfall which should be borne in mind when interpreting aspirates from salivary gland tumours.

Aged↗
PubMed1993

A CD18 antibody prevents lung injury but not hypotension after intestinal ischemia-reperfusion.

Antibodies to the neutrophil CD18 integrin have been shown to ameliorate the local effects of intestinal ischemia and reperfusion (I/R). In addition to local mucosal injury, intestinal I/R results in systemic hypotension and injury to the lungs with lung leukosequestration. This study tests the effect of a CD18 monoclonal antibody on the hypotension and lung injury after intestinal I/R. In anesthetized rabbits, the superior mesenteric artery was clamped for 60 min followed by 3 h of reperfusion. Animals were treated with saline, an anti-CD18 monoclonal antibody (R15.7 MAb), or nonspecific immunoglobulin G. Another non-ischemic group were sham controls. Neutrophil sequestration was assessed by measure of lung myeloperoxidase (MPO) and permeability by lung-to-blood concentration ratio of 125I-labeled bovine serum albumin and wet-to-dry weight ratio. Immediately after reperfusion, mean arterial pressure fell to 49 +/- 2.1 mmHg and remained at this level. The hypotension was unaffected by treatment with R15.7 MAb. Thirty minutes after reperfusion, the circulating white blood cell count fell to 2.91 +/- 0.53 x 10(3)/mm3 vs. sham 6.40 +/- 0.66 x 10(3)/mm3 (P < 0.05). Treatment with R15.7 MAb prevented this fall in white blood cell count (5.75 +/- 1.59 x 10(3)/mm3). At 3 h of reperfusion in saline-treated animals there was increased MPO, 74.8 +/- 4.9 U/g vs. 42.0 +/- 4.8 U/g in sham animals (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗