Biomedical subjects

J Hermans

Publications and source records attributed to J Hermans.

669 records · Page 38Linked to original sources

PubMedDate not supplied

Allopurinol dosage and effect on ischemia-reperfusion damage in elective and acute aortic surgery.

To study the effect of a safe dosage of allopurinol on ischemia-reperfusion damage following aortic surgery, 24 patients undergoing either elective or acute aortic reconstruction, were randomized to receive allopurinol or placebo, yielding four groups: elective/placebo (EP), elective/allopurinol (EA), acute/placebo (AP) and acute/allopurinol (AA). Blood concentrations of allopurinol, oxypurinol, uric acid, malondialdehyde, ascorbic acid, and 99mTc-albumin were determined perioperatively. Adequate concentrations and biochemical activity of allopurinol and oxypurinol were obtained, without side-effects. Malondialdehyde did not increase perioperatively, but was significantly higher in acute surgery than in elective surgery intraoperatively. Yet, ascorbic acid levels and 99mTc-albumin disappearance were not different from groups EP and EA. No influence of allopurinol was found on malondialdehyde, ascorbic acid and 99mTc-albumin. An influence of allopurinol may have been obscured, as patients in group AA were more hypotensive than in group AP. In conclusion, adequate allopurinol concentration can be obtained with a safe dosage in abdominal aortic surgery. Signs of ischemia-reperfusion injury were found in acute surgery, not in elective surgery. Therefore, further investigation on the clinical effect of allopurinol is only useful in acute aortic surgery.

Aged↗

PubMedDate not supplied

Protein expression of cancer associated genes: biopsy material compared to resection material in laryngeal cancer.

BACKGROUND: Changes in several (onco)genes and their protein expression play a role in the development of Head and Neck Squamous Cell Carcinoma (HNSCC). If protein expression is to be used for clinical purposes, their expression should preferably be evaluated during the initial diagnostic work-up when only biopsy material will be available. To investigate the correlation between assessment of expression in biopsy and resection material, protein expression in both was evaluated and compared. MATERIALS AND METHODS: In this study we compared the expression of P53, Rb protein, E-Cadherin, Ep-CAM, Desmoplakin1 and cortactin by immunohistochemistry. Expression of the proteins was studied in biopsy and resection material of 26 laryngeal carcinomas. RESULTS: A variable rate of mismatches between the scoring on biopsy and resection material was found for the different proteins. CONCLUSION: If protein expression is to be used in clinical practice and decision making it should be realized that a discrepancy exists between the expression in biopsy material and the complete resection material.

Antigens, Neoplasm↗

PubMedDate not supplied

Differences in expression of oncogenes and tumor suppressor genes in different sites of head and neck squamous cell.

BACKGROUND: In most studies concerning chromosomal changes or protein expression in head and neck squamous cell carcinomas (HNSCC) no distinction is made between the sites within this area. The behaviour of tumors arising in one site or the other, however, differs significantly, suggesting different intrinsic tumor properties. In this study we compared the expression of several proteins (p53, Rb, cyclin D1 myc, bcl-2, EGFR, neu, E-Cadherin, Ep-CAM, Desmoplakin1 and nm23) in the three major sites of HNSCC (larynx, pharynx, and oral cavity). MATERIALS AND METHODS: Expression of the proteins was studied by immunohistochemistry in 33 laryngeal, 31 pharyngeal and 36 oral carcinomas. RESULTS: Cyclin D1 had a very high level of expression in the pharynx (p = 0.0004) and EGFR very low in the larynx (p < 0.0001) compared to the other sites. To a lesser degree p53 (p = 0.05), Desmoplakin1 (p = 0.04) and Ep-CAM (p = 0.02) showed statistically nearly significant differences. For the other proteins no statistically significant difference in expression was found. CONCLUSION: These results show that the expression of a number of proteins is not identical in the three major localizations of HNSCC and indicate the different tumor biology of cancers originating from different sites in the Head and Neck. It is therefore not justified to consider the different localizations as one entity. Moreover, these differences may have clinical and prognostic relevance.

Carcinoma, Squamous Cell↗