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Biomedical subjects

J Henry

Publications and source records attributed to J Henry.

At least 127 records · Page 7Linked to original sources
PubMed1986

Tyrosine kinases in normal human blood cells. Platelet but not erythrocyte band 3 tyrosine kinase is p60c-src.

The major tyrosine kinase from platelets was purified as a 51 kDa active enzyme which was shown to be a degradation product of the protooncogene product p60c-src. Immuno-depletion experiments using a monoclonal antibody recognizing p60c-src failed to remove band 3 phosphorylating activity from red blood cell membranes. The erythrocyte tyrosine kinase was not at all immunoprecipitated by this antibody under conditions where the platelet enzyme was immunoprecipitated.

Anion Exchange Protein 1, Erythrocyte↗
PubMed1986

Cellular immune aspects of the human fetal-maternal relationship.

In the present study the number and the function of T lymphocytes obtained from peripheral blood of 35 mothers immediately after delivery and from the cord blood of the respective newborns were tested. Furthermore, the effect of the newborns' lymphocytes on the immunological competence of the mothers' lymphocytes was investigated by means of graft-versus-host-reaction xenograft test. The blood of 14 of the mothers and their newborns was also examined by monoclonal antibodies for determination of T helper and T suppressor lymphocytes. A marked reduction in the absolute number of T helper cells was found in the blood of mothers after delivery. In addition, mothers' lymphocytes manifested a high suppressor activity when added to normal human lymphocytes. It was also found that the cord blood contains lower number and percentage of T suppressor cells. Yet the T cells of the cord blood exert a high suppressor activity on the mother as well as on normal donor lymphocytes. The impaired function of cord blood lymphocytes could be impaired function of cord blood lymphocytes could be restored by in vitro addition of THF, a thymic hormone.

Female↗
PubMed1985

Characterization of human red blood cell tyrosine kinase.

A new tyrosine kinase in human red blood cells has been characterized and partially purified. The major substrate was a protein of molecular weight 93 K which could be phosphorylated both in whole red blood cells incubated with inorganic [32P] orthophosphate and in ghost preparations incubated with [gamma 32P] ATP. This tyrosine kinase displayed an alkaline isoelectric pH (around 8.5), a molecular weight of 32-33 K and does not seem to be autophosphorylable. Some kinetics of the enzyme are reported. This red blood cell tyrosine kinase is unrelated to EGF and insulin or insulin-like receptor subunits. This enzyme may represent a novel class of tyrosine kinases.

Anion Exchange Protein 1, Erythrocyte↗
PubMed1985

Protein kinases in human leukemic cells.

Protein kinase activities and cyclic AMP binding capacity were investigated in human peripheral blood cells from leukemic patients and normal controls. Using [gamma 32P] ATP as phosphoryldonor, the phosphorylating activities were not found to be significantly different in either normal or leukemic cells when measured on both artificial basic and acidic substrates. In contrast, the GTP-dependent casein kinase activity, CK2, which is almost undetectable in normal granulocytes, was markedly increased in highly proliferating myeloblastic cells from patients with acute myelogenous leukemia (AML) or with chronic myelogenous leukemia in blastic crisis (BC-CML). Levels of endogenous phosphotyrosine were not higher in leukemic cells than in normal peripheral lymphocytes or granulocytes. Finally, cAMP binding capacity was found to be increased in several types of proliferating leukemic cells, due to a higher amount of the R1-type regulatory subunit of the cAMP-dependent protein kinases. Specific patterns of cAMP binding proteins observed in the different types of normal blood cells were rather blurred in leukemic cells. In conclusion, modifications observed in human leukemic cells seem to be more related to proliferation or blockage in normal differentiation than to their cellular origin.

Adenosine↗
PubMed1985

A multivariate analysis of prognostic factors in early stage Hodgkin's disease.

A multivariate analysis of the prognostic factors was carried out with a Cox model on 1,139 patients with clinical Stage I + II Hodgkin's disease included in three controlled clinical trials. The following indicators had been prospectively registered: age, sex, systemic symptoms, erythrocyte sedimentation rate (ESR), number and sites of involved lymph node areas, histologic type, clinical stage, pattern of presentation, results of staging laparotomy when performed, as well as the date and type of treatment. A linear logistic analysis showed that most of the indicators are interrelated. This emphasizes the necessity of a multivariate analysis in order to assess the independent influence of each of them. The two main prognostic indicators for relapse-free survival are systemic symptoms and/or ESR and number of involved areas. The only significant factor for survival after relapse is age. Sex has a small but significant influence on relapse-free survival. The relative influence of each indicator varies with the type of treatment and these variations may help in understanding the biologic significance of the indicators.

Adult↗
PubMed1985

Knee problems in children and adolescents.

It is important to recognize that there are differences in the spectrum of knee injuries that occur in childhood and adolescence and to always keep these in mind when examining the younger athlete. Constant vigilance for hip pathology presenting as knee pain is also necessary in this age group. As the tendency becomes greater for practitioners to become more specialized in treatment modalities (for example, arthroscopic surgeons, knee surgeons, and so forth), the likelihood of making preventable errors in the case of young athletes increases. The comprehensive care of the athlete and his or her injury is paramount, and one should be wary of the pressure to treat the youngster as one might the college or professional athlete in order to return him or her to sports more quickly. Serious injuries require time to heal and even more time for the patient to convalesce and undergo rehabilitation.

Adolescent↗
PubMed1985

[Cancer of the endometrium and radiotherapy. Review of 280 patients treated at the Institut Bordet].

280 patients were treated at the Institute Bordet between 1963 and 1977 for endometrial carcinoma. 149 patients had radiotherapy alone, 65 patients had radiotherapy before operation and 66 patients after operation. Radiation alone was reserved for those patients whose operative risk was very serious. Five year survival was 49.6% after irradiation alone in Stage I tumours (A "packing" technique was used to deliver the radiation therapy). 22.7% of these patients died of intercurrent diseases. The majority of the recurrences were in the uterus. This series confirms the prognostic importance of the clinical staging, of the length of the uterine cavity and the degree of differentiation of the tumour. The five year survival rate after postoperative irradiation is 75%, and 84% after preoperative irradiation. Though the association of surgery with irradiation gives better results for those patients who can be operated on, it must be pointed out that radiotherapy alone gives a chance of curing an important percentage of patients whose surgical risk is in a major category.

Combined Modality Therapy↗