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Biomedical subjects

J Heim

Publications and source records attributed to J Heim.

At least 73 records · Page 4Linked to original sources
PubMed1984

A pure enzyme catalyzing penicillin biosynthesis.

Isopenicillin N synthetase (cyclase) has been purified to homogeneity from Cephalosporium acremonium strain C-10. The enzyme has a molecular weight of 40,000 to 42,000 and yields a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was purified in 10 percent yield by a combination of protamine sulfate and ammonium sulfate precipitations, gel filtration, and ion-exchange high-performance liquid chromatography. The purified enzyme can be stabilized with sucrose and stored at -20 degrees C for several weeks without any loss in activity.

Acremonium↗
PubMed1984

Repression of beta-lactam production in Cephalosporium acremonium by nitrogen sources.

A variety of inorganic and organic nitrogen sources were added to fermentation media to determine their regulatory effects on the production of beta-lactam antibiotics by Cephalosporium acremonium. (NH4)2SO4 at concentrations higher than 100 mM (1.3%) strongly inhibited beta-lactam production. L-Asparagine and L-arginine proved to be the best nitrogen sources tested for beta-lactam production. The optimum concentration of asparagine was 1.2%. Higher concentrations led to NH3 accumulation, increase in pH, and lower growth rates. Addition of tribasic magnesium phosphate [Mg3(PO4)2 X 8H2O] to the (NH4)2SO4-containing medium stimulated beta-lactam production markedly and ammonium repression of the ring-expansion enzyme was reversed. It appears that the ring-expansion step is a very sensitive part of beta-lactam biosynthesis in C. acremonium with respect to nitrogen source repression. Other enzymes may also be sensitive in view of the fact that nitrogen source derepression not only led to increases in cephalosporin C but, to a lesser extent, penicillin N and total beta-lactam titers.

Acremonium↗
PubMed1983

[Reversible hyperthyroidism caused by painless postpartum thyroiditis].

Hypothyroidism was discovered in the postpartum period. The disorder resolved spontaneously. Tests for antimicrosomial antibodies were strongly positive. These findings led to the diagnosis of painless autoimmune thyroiditis. Cases of transient or permanent hypothyroidism and hyperthyroidism in the postpartum period are reviewed.

Adult↗
PubMed1983

[Screening for incomplete enzymatic blocks of 21-hydroxylase. Study of 17-OH progesterone with tetracosactide in the follicular and luteal phases in hirsute women].

It seemed to us worth while when taking plasma samples to prove the presence or absence of ovulation and the presence or absence of a 21-hydroxylase block by estimating the levels of 17-hydroxyprogesterone under stimulation with tetracosactide. The results are as delta hydroxyprogesterone (ng/dl/mn). Eighteen women were studied in this way in the follicular phase (delta OHP: 1.21 +/- 0.71 ng/dl/mn) and 9 women in the luteal phase (delta OHP: 0.76 +/- 0.55 ng/dl/mn). This, while reliable, was not significant statistically. The explanation seems to be linked to anovulation. In the five cases with anovulation delta OHP was 0.233 ng/dl/mn, while in other cases where ovulation was proved to exist it was 1 ng/dl/mn. It seems to us that the so-called "luteal phase" should not have been retained when we wanted to test for a hormonal block, except where ovulation has been proved previously to be present, because of the possibility of anovulation. The interrelationships between the polycystic ovary syndrome and the therapeutic implications are discussed.

17-alpha-Hydroxyprogesterone↗
PubMed1983

[Androgens: a critical study of the biological diagnosis of the hirsute women. Screening for incomplete blockage of 21-hydroxylase].

In 19 patients with unexplained hirsutism without virilisation, a check-up includes delta 4- androstenedione (A), testosterone, DHEA-S. A appears like the best hyperandrogenemia parameter. Nevertheless 5 patients among this group have normal values of all these hormones. A mild congenital adrenal hyperplasia by 21 hydroxylase deficiency is revealed by abnormal response of 17 -OH-progesterone in the cosyntropin test. Our normal value of delta-OHP is, according to Gutai, method, 1.58 +/- 0.83 NG/DL/MN.

Adolescent↗
PubMed1982

[Determination of plasma 5 alpha-androstane-3 alpha, 17 beta-diol in the hirsute woman. A critical study of the biological diagnosis of hyperandrogenism].

Plasma 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) levels were measured by radioimmunoassay after separation by column chromatography in normal and hirsute women. Mean values were 106 +/- 17 pg/ml in normal women and 182 +/- 22 pg/ml in hirsute women, the difference being significant (p less than 0.001). High plasma 3 alpha-diol levels were found in 29 out of 31 patients. Measurements of testosterone (T), delta-4 androstenedione (A) and dehydro epiandrosterone sulphate were performed simultaneously in 23 patients. The 3 alpha-diol measurements proved as useful as the other parameters to assess biochemical hyperandrogenism, but they cannot replace simultaneous T and A measurements which remain the best diagnostic criteria.

Adolescent↗
PubMed1982

[Short and long term results in the treatment of obesity. A study in 173 patients (author's transl)].

Short and long term weight loss was studied in 173 obese patients treated by a low-calorie diet. Significant weight loss was observed in 78% of the patients after 6 months and in 58% after 4 years. These results are similar to those published in other studies but the comparison is difficult because the methods used are not strictly comparable. A detailed study of 116 patients showed that weight loss is not significantly influenced by such variables as age, sex, previous calorie intake, complications, and severity or duration of the obesity.

Adult↗
PubMed1982

[Relapse of Addison's disease after apparent recovery : a case-report].

In our observation, antituberculous therapy was given systematically, although neither interview nor bacteriology had provided unequivocal evidence of tuberculosis. Corticosteroid withdrawal by the patient was uneventful. After, withdrawal, the 17 OH steroids and the response to metyrapone were normal. The water load test was normal. After insulin, only cortisol levels did not increase. Salt-loss with hyponatremia then occurred spontaneously ; 17 OH steroids were low and did not increase after corticotrophin.

Addison Disease↗
PubMed1982

[Schmidt's syndrome. Interrelationships between thyroid hormones and cortisol (author's transl)].

An hypothyroidism is discovered. Secondary, a primary adrenal insufficiency is proved. Therefore, we can affirm the Schmidt's syndrome. The anti-adrenal and anti-thyroid antibodies are positive; when the glucocorticoid treatment is begun, the dose of thyroxine treatment must be decreased. That point allows us to discuss the interrelationships between thyroid and adrenals.

Adrenal Insufficiency↗
PubMed1982

[A new observation of an H-Y antigen positive 46, XX male (author's transl)].

A new serologic test for detecting the H-Y antigen by indirect fluorescence performed on purified lymphocytes was developed. It allowed us to demonstrate H-Y antigen in an 46, XX male. The presence of this antigen explains why the primitive gonad underwent testicular differentiation. Several mechanisms could account for the presence of H-Y antigen. Mosaicism or translocation of an X on a Y or an autosome may prevent detection of a Y chromosome. Alternatively, a Y chromosome may have disappeared during embryogenesis, The last hypothesis suggests that an autosome may carry a structural gene coding for H-Y antigen; in this case thye Y chromosome would act only as an accessory regulating gene.

Adolescent↗
PubMed1982

[Hypothyroidism of central origin corrected by the cessation of Triac therapy].

The association of a diffuse goiter, hypothalamo-hypophyseal hypothyroidism and iodine saturation led to the reinterrogation of an obese patient who admitted taking Triac in order to lose weight. On withdrawal of this drug, the thyroid hormones and TRH test returned to normal. Triac can therefore induce reversible hypothalamo-hypophyseal hypothyroidism.

Female↗
PubMed1981

[Late discovery of female pseudo-hermaphroditism by complete blockade 21-hydroxylase (author's transl)].

The authors report on a case where complete blockade of 21-hydroxylase was discovered in a 40-year-old subject. They emphasize the usefulness of urinary 17-ketosteroid and 17-OH progesterone assays in the diagnosis of pseudo-hermaphroditism. In contrast to major hyperandrogenism, the HY antigen test is negative, since the percentage of fluorescent lymphocytes is even lower than in the normal female population.

17-Ketosteroids↗