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Biomedical subjects

J Heil

Publications and source records attributed to J Heil.

47 records · Page 3Linked to original sources
PubMed1982

Angiotensin stimulates oxytocin release: imparied response in rats with genetic hypothalamic diabetes insipidus.

Intracerebroventricularly (i.c.v.) administered angiotensin II (ANG II) at a dose of 100 ng caused a large increase in plasma oxytocin levels in Long Evans (LE) rats and in rats heterozygous for hypothalamic diabetes insipidus (HZ). In rats homozygous for diabetes insipidus (DI) even 100 fold higher doses of ANG II i.c.v. exerted only marginal effects on oxytocin release. The impaired responsiveness in DI rats was fully restored by prolonged treatment with a vasopressin (AVP) analogue. These data show that the decreased sensitivity of ANG II receptors in DI rats is due to the AVP defect and its metabolic consequences and can be reversed by AVP substitution.

Angiotensin II↗
PubMed1982

Combined immunosuppressive therapy with cyclosporin A and azathioprine. A synergistic effect in three of four experimental models.

Cyclosporin A (Cy A) by itself is limited in its clinical application by its dose-related liver and kidney toxicity. Combining Cy A with prednisone allows lower doses of Cy A to be used, but the side effects of steroids are cumulative. Thus, we tested the effect of Cy A and various drug-dose combinations with azathioprine (AZA) in four different models. The one that gave the best results with the least toxicity was 1.25 mg of Cy A plus 15 mg of AZA for islet allotransplantation in rats across a minor histocompatibility barrier and for heterotopic heart allotransplantation in rats across a major histocompatibility barrier. In the latter, using the same strain of rats, no significant effect can be shown in rat pancreas allotransplantation. In canine renal allografts, only one dose combination (5 mg of Cy A plus 2.5 mg of AZA) was tested, but it was synergistic. Cy A may potentiate the myelosuppressive effect of AZA, and the dose may have to be adjusted to avoid this problem.

Animals↗
PubMed1982

Long-term preservation of segmental pancreas autografts.

Clinical pancreas transplantation is logistically difficult because of uncertainty concerning how long pancreas grafts can be preserved. We studied the viability of canine segmental (tail) pancreatic autografts transplanted after 24 to 72 hours of hypothermic preservation in either modified Collins' solution or modified silica gel filtered dog plasma (SGF). All grafts stored for 24 to 48 hours functioned immediately (plasma glucose less than 140 mg/dl in recipients). Grafts that failed did so between 2 and 7 days, and five dogs died with functioning grafts. The long-term functional success rate was 80% for fresh transplants; 67% and 40% for grafts stored in Collins' solution for 24 and 48 hours, respectively; and 75%, 75%, and 30% for grafts stored in SGF for 24, 48, and 72 hours, respectively. If technical failures are excluded. 50% of grafts stored in Collins' solution and 100% of grafts stored in SGF for 48 hours functioned long term. K values for the intravenous glucose tolerance test at 2 weeks in dogs with functioning grafts ranged from -1.44% to -1.78% and were similar in all groups. In conclusion, pancreatic grafts can be preserved for 48 hours by simple cold storage, but SGF is more reliable than Collins' solution (P = 0.015). Four human pancreas grafts were stored in SGF for 7 to 22 hours, one with and three without prior warm ischemia, and all three of the latter functioned after transplantation.

Animals↗
PubMed1979

Repressor synthesis in regulatory mutants of bacteriophage P22.

SDS-polyacrylamide gel analysis of P22-infected Salmonella has allowed identification of the c2 repressor (MW 31,000) and study of repressor synthesis in regulatory mutants of P22. Repressor is synthesized in reduced amounts or is absent in infections with P22, clNo.7, P22, c2 am08, P22 c3 am03, and P22 c3 am012, but is synthesized in markedly increased amounts in the virulent mutant, P22 virB3, and its component mutants, vx and k5. Higher levels of repressor are also found in the P22 cly 17 mutant.

Electrophoresis, Polyacrylamide Gel↗
PubMed1976

Effect of an extramural program of dental care for the special patient on attitudes of students.

All too frequently, courses and clinical experiences are introduced in dental education merely becausse they seem to be the popular things to do. Unfortunately, however, just as frequently such experiences are inappropriately planned and developed; they fail to achieve the basic purpose behind their introduction. This study reports such a failure in dental education. A course in dental care for the special patient was hastily and inadequately conceived and implemented. Thecourse did not achieve its objective'in fact, had it been allowed to continuein subsequent years, it could have done more harm than good. The means exist for evaluating whatever id done, and the educator should be willing to accept failure as well as success. The recognition and identification of failure can infact serve a worthwhile purpose, to the advantage of the system, if the elementscontributing to the failure are identified and proper changes are introduced.

Attitude↗
PubMedDate not supplied

Fatty acids as markers of the blood-cerebrospinal fluid barrier function in man.

The blood-cerebrospinal fluid barrier (BCB) function in man is derived from the CSF:serum ratio of albumin (QAlb). In order to study other than BCB protein transport we compared CSF:serum ratios of fatty acids in patients revealing normal and increased QAlb values. Ratios of unsaturated fatty acids, which are involved in inflammation, increased significantly in patients with increased QAlb (p < 0.005). In contrast, CSF:serum ratios of saturated fatty acids increased only slightly. We conclude that characterisation of BCB lipid properties may be of particular interest regarding drug penetration into the CSF and lipid metabolism in inflammation.

Adult↗