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Biomedical subjects

J Hauss

Publications and source records attributed to J Hauss.

At least 109 records · Page 6Linked to original sources
PubMed1986

DNA stemline heterogeneity in colorectal cancer.

The current study was carried out on 88 colorectal carcinomas to assess the degree of intratumor heterogeneity as reflected by multiple aneuploid DNA stemlines and their relation to tumor stage and morphologic differentiation. Each tumor was segregated into an average of nine specimens (3-15), which were analyzed separately. DNA aneuploidies were identified in 72 cases (82%), 29 revealing multiple aneuploid DNA stemlines with up to four aneuploid subpopulations. In 10 of the 29 carcinomas with DNA stemline heterogeneity, a ratio of 2:1 was calculated from the different DNA indices, possibly indicating that the additional DNA stemline emerged from the first one by doubling its DNA content. No correlation was found between the overall frequency of DNA aneuploidies or heterogeneous DNA stemlines and the tumor stage according to Dukes' staging. Well-differentiated carcinomas tended to express aneuploid DNA stemlines more frequently than moderately or poorly differentiated tumors, although the morphologic intratumor heterogeneity did not correspond to the appearance of multiple aneuploid DNA stemlines. These data indicate a high degree of intraneoplastic diversity in colorectal cancer and emphasize the usefulness of DNA analyses for the quantitative assessment of tumor heterogeneity.

Aneuploidy↗
PubMed1986

[High-dose piritramide basal anesthesia in experimental anesthesia and surgery].

On the basis of investigations of three groups of dogs (7 in each) the effects of high dosage piritramide anesthesia were analyzed. Using a standardized technique no significant changes in hemodynamics and microcirculation were found during 4 h. So variations in the "steady-state" must be attributed to additional medication or therapeutic intervention. High dosage piritramide anesthesia has been applied in more than 250 animal experiments.

Anesthesia↗
PubMed1985

Septicemia during pregnancy: a study in different species of experimental animals.

When Escherichia coli B6 lipopolysaccharide, 0.2 mg/kg of body weight, was infused into nonpregnant minipigs during a 5-hour period, the animals died after 12 to 16 hours as a result of endotoxic shock. When the same infusion was given to six pregnant minipigs at term, these animals died after only 3 1/2 hours. The decrease in the number of white blood cells, the number of platelets, hematocrit, and clotting factors was not significantly different between the two groups. The acid-base status, however, indicated a much more pronounced metabolic acidosis in the pregnant animals than in the nonpregnant controls. In the pregnant minipigs heart rate, cardiac output, mean arterial pressure, and total peripheral resistance indicated cardiovascular collapse, and the multiple wire, platinum surface electrode revealed a drastic reduction in uterine tissue oxygenation in the pregnant animals. The data support the hypothesis that pregnant animals at term are more susceptible to the harmful effects of lipopolysaccharide. Early death in the pregnant minipigs, however, was not associated with disseminated intravascular coagulation as it is in smaller animals (rat, rabbit, and hamster).

Animals↗
PubMed1985

Adrenalectomy in adrenal hypertension.

Between 1965 and 1983, a total of 138 patients with endocrine hypertension were treated at the Münster Surgical Clinic. 71 catecholamine-producing tumors were removed in 56 patients, 60 patients had primary aldosteronism; 32 patients with Cushing's syndrome received uni- or bilateral adrenalectomy. The operative access to the adrenals was by anterior abdominal incision which we consider superior to lateral or transthoracic approaches. 35 complications occurred. The operative management of these forms of secondary hypertension, with its low overall rate of complications, is currently seen as the most successful therapeutic approach.

Adenoma↗
PubMed1984

Steady state condition--what does it mean during investigation of oxygen supply?

Judging from the presented data it was possible to maintain steady state conditions during high dose piritramide anesthesia for a period of three and a half hours. Under these carefully defined experimental conditions it will be possible to actually correlate any changes observed in the microcirculatory bed with medication or other interventions. So-called "normal Po2 histograms" taken from different organs of dogs under the above-defined experimental conditions (basic anesthesia with high-dose piritramide, controlled artificial ventilation with 21% O2) are presented in Fig. 12. We feel that any comparison of results from different working groups must be based on exactly defined and standardized experimental conditions, because otherwise the methodological differences would prevent adequate comparability.

Animals↗
PubMed1983

Cultivated human arterial smooth muscle cells. The effect of donor age, blood pressure, diabetes and smoking on in vitro cell growth.

The growth capacity and morphology of cultured human smooth muscle cells obtained from omental arteries from 153 patients were studied. The cells exhibited a very slow growth rate compared to rat or minipig SMC's, but they too developed cultures in a typical hill-and-valley pattern and possessed bundles of myofilaments. Two groups of smooth muscle cell cultures, slowly proliferating ones and rapidly proliferating ones were compared in their relation to donor age, blood pressure, nicotine abuse, diabetes mellitus and carcinoma. In the case of blood pressure and carcinoma significant differences indicated that those smooth muscle cells obtained from patients with high blood pressure or carcinoma revealed lower growth capacities. In the second subculture the proliferation of smooth muscle cells was found to be inversely proportional to donor age (4 to 78 years).

Adult↗
PubMed1983

Comparative pO2 histograms and other parameters in canine kidneys during perfusion with Oxypherol.

Our experiments were performed under the aspect of kidney perfusion in vivo. Our goal was to find a medium which would help preserve the potential donor kidney within the cadaver until the time of explantation. A perfusion medium must be chosen that transports oxygen and releases it in the organ to be preserved, so that the metabolic processes continue without damage to the tissue. From our results we believe that the Oxypherol medium meets these requirements. The measurement of local oxygen pressure by the multiwire surface electrode according to Kessler and Lubbers (1966) gives a reliable parameter for judging organ vitality.

Animals↗
PubMed1982

[In vitro studies of cells in histiocytosis X].

In a female patient with histiologically ensured histiocytosis X, cell cultures modified according to NEZELOF were performed in methylcellulosis and Eagle's medium. From the 5th to the 15th day of incubation the cells were daily observed natively through a normal microscope and taken for histochemical examinations and for checking their capacity of phagocytosis. Two cell types could be distinguished: spindle-shaped fibroblasts as well as round cells of changing form and size, the alpha-naphthylacetate esterase response of which proved to be positive. The round cells can partly be regarded as HK cells, whereas giant cells could be predominantly observed during the second week. A phagocytosis of iron chips and human erythrocytes loaded with antibodies could be revealed in both types. HK cells represented mature macrophages, from which giant cells developed under in vitro conditions. There is still not explanation as to the local and immunological factors characterizing the clinical picture of histiocytosis X.

Adult↗
PubMed1980

Rupture of the aortic wall with compression of bronchial arteries.

A case of closed traumatic rupture of the aortic wall with compression of the bronchial arteries is described. No comparable case has been reported in the literature so far. The effect on pulmonary perfusion and gas exchange is demonstrated. The compression, displacement and transportation of the bronchial arteries (vasa privata) is shown is postmortem angiography.

Adult↗
PubMed1979

[Superselective parathyroid hormone determination--a reliable diagnosis of the location of endocrinological active parathyroid neoplasms].

The importance of superselective catheterization of the small jugular veins for PTH-radioimmunoassay is shown as an improvement of the preoperative localization technique. Since May 1976 in 20 patients the diagnosis of pHPT could be established. Our own experiences with the method of small vein sampling combined with the radioimmunoassay demonstrate that a reliable localization of overactive parathyroid tumours is possible.

Humans↗
PubMed1979

[Experimentally induced pancreatitis (author's transl)].

Pancreatitis was produced in 29 mini-pigs by partial occlusion of the pancreatic duct; occlusion was performed by implanting a T-drain into the duct. Inflammatory changes were induced additionally by applying radiopaque material in excess. Acute and chronic pancreatitic lesions could be diagnosed by X-ray examination, evaluation of blood chemistry and by histology.

Amylases↗
PubMed1978

[Preoperative location of parathyroid adenomas using supraselective parathyroid hormone determination in the blood of the cervical veins].

After a short presentation of advances in biochemical and clinical analysis of primary hyperparathyroidism, the importance of superselective catheterization of the small jugular veins for PTH radioimmunoassay is shown as an improvement of the preoperative localization technique. Since May 1976, the diagnosis of pHPT could be established in 20 patients. Our own experiences with the method of small vein sampling combined with the radioimmunoassay demonstrate that a reliable localization of overactive parathyroid tumors is possible.

Adenoma↗