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Biomedical subjects

J Hardie

Publications and source records attributed to J Hardie.

At least 109 records · Page 6Linked to original sources
PubMed1985

Cross-reactions between alpha-streptococci and Omniserum, a polyvalent pneumococcal serum, demonstrated by direct immunofluorescence, immunoelectroosmophoresis, and latex agglutination.

In recent years several groups have used serological methods to demonstrate pneumococcal capsular antigens in sputum. In the present study 123 strains of alpha-hemolytic streptococci (including 97 strains from sputum or pharyngeal specimens) were tested for cross-reactions with a polyvalent antipneumococcal serum (Omniserum). Representatives of the following species were included: Streptococcus bovis, S. equinus, S. intermedius, S. lactis, S. milleri, S. mitis, S. mutans, S. sobrinus, S. salivarius, S. sanguis, S. suis, and Aerococcus viridans. Serological reactions were detected by direct immunofluorescence, immunoelectroosmophoresis, and latex agglutination. Fifteen (12%) of the strains gave positive reactions by all three methods. Positive reactions were also observed with another 32 strains (26%) with two of the methods, whereas 37 strains (30%) gave positive reactions by just one technique. Altogether 84 (68%) strains gave positive reactions with one or more of the methods. Latex agglutination gave positive reactions with 26 (21%) strains compared with 57 (46%) in immunofluorescence and 63 (51%) in immunoelectroosmophoresis. Absorption of the antiserum with one alpha-hemolytic strain reduced but did not entirely eliminate the cross-reactions with five tested strains. These findings indicate a potential risk of cross-reactions with polyvalent antipneumococcal serum in tests carried out on sputa or other specimens which may be contaminated with alpha-hemolytic streptococci.

Cross Reactions↗
PubMed1985

Pretreatment with chemotherapy in patients with advanced head and neck cancer.

Average survival for advanced head and neck cancer (AHNC) is 18 months. In an attempt to improve this we treated 29 AHNC patients between 1978-82 with two courses of chemotherapy. Chemotherapy consisted of cyclophosphamide, methotrexate, 5 fluorouracil and bleomycin; or bleomycin, cisplatinum and methotrexate. Chemotherapy was given prior to definitive therapy of radiotherapy or radiotherapy and surgery. All patients were stage 3 or 4. All patients were Eastern Co-operative Oncology Group status performance 0 or 1. Response to chemotherapy did not improve survival. Pretreatment with chemotherapy should be investigational until increased survival has been documented.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1985

A 1985 update on AIDS.

Explore the source record for details and available documents.

Acquired Immunodeficiency Syndrome↗
PubMed1984

Mucoepidermoid carcinoma: ultrastructural and histogenetic aspects.

Ultrastructural examination of 6 mucoepidermoid carcinomas (4 from parotid salivary gland and 2 from lung) was used to investigate tumor cell types, their interrelationship, histogenetic aspects and factors involved in the multiplicity of histological patterns in this tumor. On the basis of light-microscopic cellular features, mucoepidermoid carcinomas are generally thought to be formed by 3 principal cell types, mucinous, squamous and intermediate. However, low-magnification electron micrographs reveal that there are 2 basic tumor-cell types interpreted as modified luminal epithelial and myoepithelial cells. Both cell types may independently show a range of fine structural modification and organizational arrangement. Luminal epithelial cells have varying degrees of mucin production and lumen formation in some regions, while modification of these cells is one mechanism for squamoid and frankly squamous appearances in other areas. Preferential proliferation of the second type of tumor cell aligned on the outer aspect of luminal epithelial cells is responsible for the development of isomorphic regions composed of intermediate cells. Squamous metaplasia can also occur in relationship to these latter cells. Based on these observations and recent ultrastructural and histochemical studies of salivary gland tumors, it is suggested that, in addition to the excretory duct, neoplastically altered cells in the acinar-intercalated duct region must be considered as a potential source for the development of mucoepidermoid carcinomas.

Adult↗
PubMed1982

The three-dimensional structure of the Z disc in insect supercontracting muscles.

Two type of Z disc structure have been reported in insect supercontracting muscle fibres: (i) a perforated Z disc where Z material forms a reticulum and (ii) a fragmented Z disc composed of separate, discrete Z bodies. The use of thick (1 micron) sections in conjunction with high voltage electron microscopy can distinguish between these two types while conventional thin sections may lead to misinterpretation of structure. It is shown that in one insect, the crane-fly Tipula, the larval body-wall muscles, for which a fragmented Z disc has been proposed, do in fact have a perforated disc. In the wax moth Galleria, homologous muscle fibres have a similar type of Z disc, a finding which indicates the need for re-examination of other lepidopteran muscles claimed to have fragmented discs. A redefinition of supercontraction is proposed which includes reference to the perforated type of Z disc.

Animals↗
PubMed1980

Z disc expansion during supercontraction: a passive mechanism?

The hypothesis of active Z disc expansion during supercontraction is re-examined using larval blowfly body-wall muscles. The previous results on glycerinated supercontracting fibres are confirmed. However, further observations on whole fibres stimulated to contract artifically and on skinned muscle fibres, as well as certain theoretical considerations, have led to the postulation of a passive mechanism. This mechanism is dependent upon mechanical continuity of the Z discs across the fibre.

Animals↗
PubMed1979

Hospital admission rates of diabetic children.

In 1973 a children's diabetic clinic was established in Oxford. An analysis has been made of the effects this has had on the pattern of emergency admissions classified as severe hyperglycaemia (A), moderate hyperglycaemia (B) and hypoglycaemia (C). A comparison of the admission rate per "diabetic year" over the four years before and the three years after the children's clinic was established showed a significant decrease in admission rate for all hyperglycaemic admissions (p less than 0.05). This decrease was shown to be mainly due to a reduction in admissions with severe hyperglycaemia (A) (p less than 0.01), there being no significant change in admission rate for moderate hyperglycaemia (B). There was no significant change in admission rate for hypoglycaemia (C).

Age Factors↗
PubMed1979

Ultrastructural studies of intraoral verruca vulgaris.

The literature offers conflicting views regarding the existence of verruca vulgaris in the oral cavity. In an attempt to clarify this issue, a series of ten oral lesions which had been diagnosed as verruca vulgaris and ten oral lesions diagnosed as squamous-cell papilloma were examined ultrastructurally. Six of the lesions diagnosed as verruca vulgaris contained the characteristic intranuclear viral particles which are normally found in that lesion. None of the lesions diagnosed as squamous-cell papilloma exhibited this type of intranuclear viral inclusions.

Cell Nucleus↗
PubMed1978

Epithelium--connective tissue interface of oral mucous membranes.

The interface between the epithelium and connective tissue of mucous membranes and skin represents a tissue site that is affected by a host of diseases which alter its normal morphologic features. Many differences exist in the normal morphologic pattern of this interface between various anatomic sites. It is essential, therefore, to have a thorough understanding of these differences before a meaningful study and assessment of pathologic changes affecting the interface can be made. This study was undertaken to establish a set of morphologic standards which can be used as a basis for interpreting pathologic changes which occur at the epithelium--connective tissue interface of oral mucous membranes.

Animals↗