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J Hansz

Publications and source records attributed to J Hansz.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

[Capacity of erythroid progenitor cells of the human bone marrow for clonal growth in diffuse chamber culture].

The ability of human marrow erythroid progenitors growth in diffusion chamber culture has been assessed. Chambers containing cells (2 X 10(5)) suspended in clot were implanted into peritoneal cavity of anemic rats. The anaemia was induced by phenylohydrazine (50 mg/kg body weigh). Erythroid colonies were counted on day 1, 2, 3, 5, 7, 9, 11, 13 and 15. Two types of colonies formed by erythroid progenitors (BFU-D-E, CFU-D-E) were determined using criteria of colony size and culture duration. The count of colonies formed by CFU-D-E was highest on day 2 (29 +/- 4) and underwent reduction in subsequent days. Colonies formed by BFU-D-E were seen beginning of day 5 and their number peaked on day 7 and 9. These results indicate that the best time for determining the growth of human marrow CFU-D-E and BFU-D-E in diffusion chamber culture is on day 2 and day 7-9, respectively.

Animals↗
PubMedDate not supplied

[Neutrophils do not affect clonal growth of granulocyte progenitor cells cultured in vivo in diffuse chambers].

The effect of polymorphonuclear leukocytes on the growth of granulocytic progenitors in the diffusion chamber culture (CFU-dG) has been studied. It has been shown that peripheral blood neutrophils do not affect the myeloid colony formation in this culture system. The inhibition of CFU-dG growth, observed in the presence of peripheral blood leukocytes was dependent on prostaglandins release from monocytes.

Animals↗
PubMedDate not supplied

[Proliferative activity of granulocyte progenitors from the bone marrow of patients with chronic myeloid leukemia in 14-day culture in vivo].

The proliferative activity of marrow granulocyte progenitors (CFU-dG) from patients with chronic myeloid leukemia (CML) and healthy subjects was investigated in 14th-day culture using diffusion chamber technique in vivo. On each day the number of clusters (20-50 cells) and colonies (over 50 cells) was estimated. In addition, on the day 4, 7 and 9 the count of CFU-dG in the S-phase of cell cycle was determined. During the first 9 days the number of clusters and colonies successively increased both in CML and control, but this result was more pronounced in experiments with CML-CFU-dG. In the period between days 9 th and 14th a decrease in the proliferative activity of CFU-dG could be observed. During the whole culture-time the number of colonies was markedly higher than that of clusters. The percentage of CML-CFU-dG in S-phase was comparable to that noted in controls.

Adult↗
PubMedDate not supplied

[Effect of activated monocytes on the growth of cloned granulocytic progenitor cells].

The humoral effect of resting and phorbol myristate acetate (PMA) preincubated normal monocytes on granulocytic progenitor growth in double diffusion chamber culture (CFU-dG) was assayed. The activation of monocytes by PMA was confirmed in NBT reduction test (increased formazan content) and by enhanced superoxide anions production compared with resting cells. The activatory and inhibitory effect of monocytes on granulocytic colony formation was cell-concentration dependent. The maximal CFU-dG growth promoting effects was seen at 5 X 10(4) of resting monocytes, whereas PMA-treated cells exerted their optimal stimulatory activity at lower cell number (2.5 X 10(4)). Further increase of monocyte concentration resulted in diminished CFU-dG proliferation. It is shown that the decline of colony count at high monocyte number was caused by indomethacin-dependent inhibitors.

Adult↗
PubMedDate not supplied

[Formation of mixed colonies by progenitor cells of chronic myeloid leukemia cultured in vivo].

The clonal growth of multipotential progenitor cells of chronic myeloid leukemia (CML) in diffusion chamber implanted into peritoneal cavity of neutropenic and anemic rats was assessed. CML precursors formed in methylcellulose culture enriched with medium conditioned by phytohemagglutinin activated lymphocytes mixed neutrophilic-erythroid colonies, in number significantly higher then normal cells. Mixed colonies produced by CML cells, in contrast to the normal precursors, contained also macrophages, eosinophils and megakaryocytes. We conclude that modified diffusion chamber culture technique may be a suitable tool for multipotential progenitor study in CML.

Adult↗
PubMedDate not supplied

[Granulocyte adherence in advanced Hodgkin's disease and its dependence on antiproliferative drugs used].

In 36 patients with stage III and IV of Hodgkin's disease granulocyte adherence was investigated. The determinations were carried out in minicolumns filled with nylon fibres. In 13 patients the determinations were repeated 2, 4, 6, 12 and 24 hours after administration of vinblastine and cyclophosphamide. In all patients granulocyte adherence was reduced, by 54.1 +/- 3.5% on the average. The values of adherence in the control group were of the order of 83.7 +/- +/- 4.7%. Administration of antiproliferative drugs reduced further the adherence. The lowest value of it was observed 6 hours after vinblastine and cyclophosphamide administration. After that time the adherence increased gradually reaching the initial level 24 hours after administration of antiproliferative agents. These results suggest that reduced adherence of granulocytes may be an additional cause, besides abnormal immunological reactions, of the impairment of the resistance mechanisms of the organism in advanced stages of Hodgkin's disease.

Adult↗
PubMedDate not supplied

[Phytohemagglutinin-stimulated lymphocyte proliferation in a diffusion chamber in vivo].

Lymphocyte proliferation stimulated with phytohaemagglutinin (PHA) was studied in vivo. For control aims experiments were done also lymphocytes without PHA. In the investigations the technique of diffusion chamber was used. Chambers with lymphocytes (1 X 10(6) cells in a chamber) were implanted into the peritoneal cavity of mice. On the 1st, 3rd, 6th, 10th, 13th, 17th and 29th days of culture the number of cells and the proportions of blast cells and small lymphocytes were determined. It was demonstrated that peripheral blood lymphocytes exposed to PHA proliferated in vivo. This was expressed initially as a rise in the blood cell count and this rise decreased more slowly than the number of cells in non-stimulated cultures.

Animals↗
PubMedDate not supplied

[Clinical usefulness of determination of the sensitivity of myeloblastic leukemia cells to antiproliferative drugs in cell culture in vivo].

In 15 patients with acute myeloblastic leukaemia and 4 with acute myelomonocytic leukaemia the sensitivity of blast cells was determined to cytosine arabinoside and and rubidomycin in cultures in diffusion chambers implanted into irradiated mice. The degree of sensitivity of leukaemic cells to antiproliferative drugs was determined after incubating the samples with cytosar and cerubidine on the basis of blood cell count changes on the 1st, 3rd and 6th days of the culture as compared with control determinations. The results of culture tests were confronted with the clinical condition of the patients. It was found that in cultures with the cells of the patients, in whom remission was achieved the number of myeloblasts decreased after incubation with cytosar and cerubidine from the 1st or 3rd day on. In patients refractory to treatment in most determinations the number of leukaemic cells incubated in cultures with the drugs remained at the level of the values obtained in control samples or after an initial slight fall this number increased again.

Adolescent↗
PubMedDate not supplied

[Clonal growth of granulocytic progenitor cells in diffusion chambers in vivo].

Investigations on colony forming ability of committed granulocytic progenitors from bone marrow were carried out. In all, 85 cultures were performed. The bone marrow was obtained from 12 subjects without evident haematological abnormalities. Cultures were carried on for 9 days in 0.2% agar medium in diffusion chambers implanted into the peritoneal cavity of irradiated mice. It was found that committed granulocytic stem cells produced on the average 132 colonies (70--240), which were divided into four types according to their size and morphology. All types of colonic consisted of granulocytes, including polymorphonuclears. In cultures also were observed aggregations of cells containing monocytes.

Adult↗