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Biomedical subjects

J Hamburger

Publications and source records attributed to J Hamburger.

At least 91 records · Page 5Linked to original sources
PubMed1980

[Production of a mastocyte growth factor in lymphocyte cultures stimulated by a mitogen or by allogenic cells].

Supernatants from mouse spleen leukocytes stimulated by concanavalin A or from a secondary MLC between donor and recipient of a skin allograft can induce in bone marrow cells an exponential growth of mononuclear cells with cytoplasmic basophilic granules. These cells can be maintained in liquid suspension culture for up to 6 months. They lack the properties of T or b lymphocytes, monocytes or macrophages. They have the morphological features of mast cells or basophils; the cytoplasmic granules are metachromatic for toluidine blue, alcian blue-positive at pH 0.3 and contain histamine. In addition, these cells bind monoclonal mouse IgE. They may be considered as cells of the basophil-mast cell lineage.

Animals↗
PubMed1979

[Production of urea during mixed leukocyte culture (MLC). Effect of presensitization by a skin allograft].

An increased production of urea has been demonstrated during mixed cultures between allograft donor and recipient leukocytes. This phenomenon may also be observed during primary MLC (i.e, without previous allograft) but to a lesser degree. This increased production of urea during MLC results from an increase of arginase activity in spleen cell population(s) resulting in an increased arginine transformation into urea and ornithine, as demonstrated by the release of an amount of ornithine equivalent to that of urea in the culture supernatant.

Amino Acids↗
PubMed1978

[Functional inhibition of isolated pancreatic cells, new technic for the detection of macrophage cytotoxicity].

It is usually accepted that macrophages "activated" by lymphokines may be found cytotoxic against tumoral target cells but show no detectable cytotoxicity in in vitro tests using normal non tumoral cells as target cells. These data have been obtained mainly with the chromium-release test. The present paper describes a new test using normal isolated pancreatic cells as target cells and evaluating the effect of activated or non-activated macrophages on the insulin secretion response to glucose stimulation. The results show a striking decrease in this response following an 18-hr incubation of pancreatic islet cells with activated macrophages, as compared to that of the same cells incubated with control macrophages. This is clear evidence that activated macrophages may alter normal cells and suggests that their cytotoxic properties are not restricted to tumoral target cells.

Animals↗