Collaborative assay of EEC standards for bovine tuberculins.
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Biomedical subjects
Publications and source records attributed to J Haagsma.
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An outbreak of type B botulism in cattle, caused by feeding grass silage, is reported. The clinical features were completely identical with those in cases of type B botulism, which occurred when abnormal brewers' grains were fed. 36.5 mouse LD50 type B toxin was found to be present in each gramme of grass silage, the bacterial count being 10(4) Cl. botulinum type B per gramme.
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An outbreak of botulism in four yearlings of a single herd is reported. One animal recovered spontaneously. The other animals underwent euthanasia (animal A) or were slaughtered (animals B and C). Cl. botulinum toxin type C was detected in the sera of animals A and B as well as in the liver and abomasal contents of animal A. In addition, Cl. botulinum was isolated from the liver of animal A. The symptoms are described. Total muscular weakness, also resulting in lingual paralysis and inability to swallow, was the most important clinical feature. The mechanism of action of Cl. botulinum toxin, possible methods of treatment and aspects of meat inspection are discussed.
An outbreak of type C botulism in cattle is reported, which was caused by the fact that litter from a broiler farm, in which a large number of toxic carcases had been present, had been used as a bedding in the cubicles. There is evidence to suggest that there is a relationship with the large number of cases of type C botulism in waterfowl in the Netherlands in recent years.
In 1970-1972 a great number of mink died of a meningo-encephalitis in the Netherlands. The results of the detailed microbiological and histopathological examination pointed to infection by NCD virus as the cause of the mortality. During the isolation of the NCD virus in embryonated hen eggs the haemagglutinating properties were extremely weak and incomplete; the normal haemagglutination of the NCD virus was not found until the mink isolates had been passed through chickens. Transmission experiments revealed that the pathogenicity of the NCD virus was relatively low in mink after i.c. and i.m. infection, very young mink being the most susceptible. The mink were doubtlessly infected with the NCD virus bij feeding with offal of infected poultry. There was in fact quite a high incidence of Newcastle disease on Dutch chicken farms in 1970-1972.
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Two cases of Aujeszky's disease in a cat and a dog belonging to the same owner are reported. The two animals each were five months of age. The symptoms shown by the cat were typical of Aujeszky's disease: intense itching, salivation and the head bent to one side. The main symptoms shown by the dog consisted in salivation, ptosis of one eye, a drooping ear, the head bent to one side and ataxia. As itching was not observed in the dog and the animal had spent the first months of its life in wooded surroundings, it could also have been affected with rabies, although it had been inoculated with LEP-Flury vaccine forty days prior to importation. It is of importance to the practitioner to know that itching may be absent in dogs with Aujeszky's disease and that rabies should also be suspected in these cases. Only a laboratory diagnosis will be conclusive. Studies were negative for rabies, the virus of Aujeszky's disease being found to be present in the two cases. The source of infection probably consisted in contaminated pork offal (larynges).
This study has shown for the first time that fish such as eels (Anguilla anguilla L.) and carp (Cyprinus carpio L.) are sensitive to type E botulinum toxin. With oral administration the MLD for eels was 27.500 mouse LD50 per kg body weight, while for carp it was only 236 mouse LD50 per kg. Carp were also found to be sensitive to a slight degree to type C botulinum toxin, the MLD of the most virulent toxin of this type being 457,000 mouse LD50 per kg with oral administration. Eels, however, showed no sensitivity to type C toxin.
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