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Biomedical subjects

J H Yang

Publications and source records attributed to J H Yang.

At least 127 records · Page 7Linked to original sources
PubMed1986

Computer prediction of peptide maps: assignment of polypeptides to human and mouse mitochondrial DNA genes by analysis of two-dimensional-proteolytic digest gels.

We have prepared a computer program that predicts complete and partial peptide maps from amino acid sequences. The program fragments amino acid sequences at designated cleavage sites and calculates the molecular weight and relative labeling of each peptide. These data are graphed as log molecular weight of the original protein (X-axis) vs. log molecular weight of the component peptides (Y-axis). The program is interactive, permitting adjustment of a number of graphic parameters and alteration of the position of proteins in the first dimension to accommodate aberrations in protein mobility. The program has been used to predict the V8 protease peptide maps of the 13 open reading frames (ORFs) identified in the human and the mouse mitochondrial DNA (mtDNA) sequences. The results were compared to the V8 protease peptide maps obtained for mouse and human mitochondrially synthesized proteins by two-dimensional proteolytic digest gels. A high correlation was observed between the predicted and observed peptide maps. These results suggest the assignment of several proteins to mtDNA genes.

Amino Acid Sequence↗
PubMed1985

The use of microcomputers for the quantitation of light intensity patterns using digitized video signals.

We have developed an inexpensive yet versatile microcomputer-based system for quantitating light intensity levels in autoradiographs. This system employs a standard video camera interfaced to an analog-to-digital convertor. A program has been written for this system which can measure intensities within a defined region of an autoradiograph, permitting an easy and accurate quantitation of spots or bands of irregular shape.

Algorithms↗
PubMed1984

Computer selection of oligonucleotide probes from amino acid sequences for use in gene library screening.

We present a computer program, FINPROBE, which utilizes known amino acid sequence data to deduce minimum redundancy oligonucleotide probes for use in screening cDNA or genomic libraries or in primer extension. The user enters the amino acid sequence of interest, the desired probe length, the number of probes sought, and the constraints on oligonucleotide synthesis. The computer generates a table of possible probes listed in increasing order of redundancy and provides the location of each probe in the protein and mRNA coding sequence. Activation of a next function provides the amino acid and mRNA sequences of each probe of interest as well as the complementary sequence and the minimum dissociation temperature of the probe. A final routine prints out the amino acid sequence of the protein in parallel with the mRNA sequence listing all possible codons for each amino acid.

Amino Acid Sequence↗
PubMed1983

Absolute configuration of (-)anisodine (a new ganglio blocking agent) and (-)anisodinic acid.

A new tropane alkaloid, (-)anisodine (I), was isolated from the plant of Anisodus tanguticus. It is a novel ganglio blocking agent. The absolute configuration of (-)anisodinic acid (VII), which is the side chain of (-)anisodine, was determined by chemical correlation with (R) (-)-2-phenyl-1, 2-propanediol (IV) and (R) (-)-2-hydroxy-2-phenylpropionic acid (VI). Thereby (-)anisodine with the absolute configuration shown as (I) is 6 beta, 7 beta-epoxy-1 alpha H, 5 alpha H-tropan-3 alpha-ol (S) 2'-phenyl-2', 3'-dihydroxypropionate. Two new tropane alkaloids; 6 beta, 7 beta-epoxy-1 alpha H, 5 alpha H-tropan-3 alpha-ol 2',3'-epoxypropionate (III) and 2'-phenyl-2'-hydroxypropionate (V) were prepared and its physical constant is reported in this paper.

Ganglionic Blockers↗
PubMed1983

Studies on antihepatitic drugs. Total synthesis of (+/-)schizandrin C and its analogs.

This paper reports the total synthesis of (+/-) schizandrin, C, namely 5,6,7,8-tetrahydro-1, 12-dimethoxy-2, 3, 10, 11-bismethylenedioxy-6, 7-cis-dimethyldibenzo (a, c) cyclooctene (12B), a new active SGPT lowering principle isolated from the Chinese medical plant Schizandra chinensis, and its 6, 7-trans-dimethyl isomer (16B). We also synthesized two more isomers, namely 5, 6, 7, 8-tetrahydro-3, 10-dimethoxy-1, 2, 11, 12-bismethylenedioxy-6, and 7-cis-(and trans-) dimethyldibenzo (a, c) cyclooctene (12A and 16A). The NMR, UV and mass spectra of these four isomers are discussed. IR (in chloroform), UV, NMR and MS of synthetic schizandrin C (12B) are identical with those of the natural compound.

Animals↗