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Biomedical subjects

J H Reid

Publications and source records attributed to J H Reid.

34 records · Page 2Linked to original sources
PubMed1991

Use of percutaneous nephrostomy in malignant ureteric obstruction.

Seventeen patients with malignant disease underwent percutaneous nephrostomy to relieve renal failure secondary to ureteric obstruction. Renal function improved in 88%. The median survival time was 18 weeks and 58% left the primary hospital to return home or to a terminal care hospice. Minor complications occurred in 58%. The use of bilateral nephrostomy tubes conferred no greater benefit than a unilateral tube. Intervention in malignant ureteric obstruction may confer some benefit and should not necessarily be viewed with pessimism.

Acute Kidney Injury↗
PubMed1989

Fibromuscular dysplasia of the renal artery in a child: detection by Doppler ultrasound and correction by percutaneous transluminal angioplasty.

We describe the case of a fourteen-year old girl with hypertension, in whom renal arterial stenosis was identified by Doppler ultrasound examination, with subsequent demonstration of typical features of fibromuscular dysplasia by selective renal arteriography. Percutaneous transluminal balloon angioplasty was performed, and a sustained improvement in renal arterial flow was confirmed by follow-up Doppler ultrasound examinations.

Adolescent↗
PubMed1989

Characterisation of monoclonal antibodies for detection of Mobiluncus spp. in genital specimens.

Monoclonal antibodies were raised to the anaerobic curved rods, Mobiluncus curtisii subsp. curtisii NCTC 11656, M. curtisii subsp. holmesii NCTC 11657 and M. mulieris NCTC 11658. Three antibodies reacted with the two subspecies of M. curtisii and, when used in combination against clinical isolates, showed 100% sensitivity and specificity in immunofluorescence studies. Immunoblotting showed that two of these antibodies reacted with an epitope on a protein which had an electrophoretic mobility corresponding to a Mr of 75 Kda in the absence of a reducing agent and 82 Kda in its presence in both type strains and in clinical isolates. The third antibody reacted with an epitope in type strains which had a mobility corresponding to 75 Kda and was unaffected by a reducing agent. However, in clinical isolates the epitope was present on a protein of 75 Kda or 71 Kda, or on both. A fourth antibody showed reactivity with M. mulieris NCTC 11658 alone, but only 6 (24%) of 25 clinical isolates gave positive results by immunofluorescence. The epitope is believed to be present on a protein of greater than 90 Kda. All four antibodies were shown by immunogold staining to be directed against epitopes exposed on the cell surface.

Antibodies, Bacterial↗
PubMed1987

Immunochemical characterization of a polysaccharide antigen of Bacteroides fragilis with an IgM monoclonal antibody.

An IgM mouse monoclonal antibody (McAb) Bf4 was produced to a surface polysaccharide of Bacteroides fragilis NCTC 9343. Immunoblotting showed that McAb Bf4 reacted strongly with a high molecular mass structure which was sensitive to oxidation with periodate but resisted protease treatment. An inhibition enzyme-linked immunosorbent assay (ELISA) indicated that McAb Bf4 did not cross react with the sixteen Bacteroides species and strains tested. Cells of B. fragilis NCTC 9343 recovered from the various interfaces of a Percoll discontinuous density gradient were tested in the inhibition ELISA. Bacteria from the 0-20%, 20-40% and 40-60% interfaces inhibited the ELISA; however, cells from the 60-80% interface did not. Electron microscopy with immunogold labelling showed that McAb Bf4 did not react with the extracellular fibrous network on bacteria recovered from the 0-20% interface, or the extracellular electron dense layer on cells from the 60-80% interface; however, it was associated with a surface structure on cells from the 20-40% interface. Growth in vivo did not enrich for bacteria with this structure.

Antibodies, Monoclonal↗
PubMed1986

Capsulation of in vitro and in vivo grown Bacteroides species.

By centrifugation on a four step Percoll density gradient cells of Bacteroides species could be separated according to the size of extracellular structure. The difference in size was visible by both light and electron microscopy. Two structures were observed on Bacteroides fragilis by electron microscopy, namely a fibrous network and an electron dense layer. An electron dense layer was visible on Bacteroides ovatus only when stained with ruthenium red. B. fragilis cells grown in the mouse peritoneal cavity did not produce a large fibrous network. An electron dense layer was observed on some cells in the presence of ruthenium red stain and cells possessing this layer were phagocytosed in vivo.

Animals↗
PubMed1984

The growth and survival of capsulate and non-capsulate Bacteroides fragilis in vivo and in vitro.

The growth of capsulate and non- capsulate Bacteroides fragilis in chambers implanted in the mouse peritoneal cavity was compared. Capsulate and essentially non- capsulate (less than 1% capsulate ) populations of B. fragilis strains NCTC9343 and NCTC10584 consistently grew exponentially to greater than 10(9) cfu/ml within 24 h in vivo, and low numbers of capsulate bacteria were maintained in the essentially non- capsulate population; however, the degree of capsulation of the capsulate population decreased by more than 60%. B. fragilis ATCC23745 differed from strains NCTC9343 and NCTC10584 in that growth was unpredictable and only occurred in some of the implanted chambers. Capsule production by cells of strain ATCC23745 varied from chamber to chamber: sometimes the proportion of capsulate cells increased after prolonged implantation. This could occur with either an increase or decrease in viable numbers in vivo and also after in-vitro incubation of this strain in chambers. The survival of capsulate and non- capsulate B. fragilis strains NCTC9343 and ATCC23745 was compared in aerobic and anaerobic conditions in vitro. In anaerobic conditions, capsulate and non- capsulate strain NCTC9343 survived equally well, whereas capsulate ATCC23745 survived better than its non- capsulate variants. Capsulate populations of both strains survived better than non- capsulate in aerobic conditions.

Aerobiosis↗
PubMed1984

Phagocytic and serum killing of capsulate and non-capsulate Bacteroides fragilis.

The relative susceptibilities of capsulate and non- capsulate variants of Bacteroides fragilis to serum and phagocytic killing were investigated. The capsule of B. fragilis did not confer resistance to serum killing. Phagocytic killing of non- capsulate B. fragilis occurred at bacterial concentrations of 1 X 10(6) and 1 X 10(7) cfu/ml. Capsulate B. fragilis organisms were also phagocytosed and killed at a concentration of 1 X 10(6) cfu/ml, but phagocytosis and killing were impaired at a concentration of 1 X 10(7) cfu/ml.

Bacteroides fragilis↗
PubMed1981

An intracellular structure in Bacteroides fragilis.

An unusual intracellular circular or oval structure was discovered during a morphological study of Bacteroides fragilis by electronmicroscopy. The structure is complex, composed of multiple concentric layers surrounding a central region. The dimensions and profile are consistent in all the sections observed. The sections illustrated may represent various stages in the morphogenesis of the complete structure.

Bacteroides fragilis↗