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Biomedical subjects

J H Perrin

Publications and source records attributed to J H Perrin.

99 records · Page 6Linked to original sources
PubMedDate not supplied

Postcolumn chemiluminescence as a detection technique in the liquid chromatographic analysis of beta-lactam ring containing compounds.

A broad range of compounds containing the beta-lactam ring were screened for enhancement of luminol chemiluminescence. A selection of these compounds were found to enhance the chemiluminescence of luminol. A high-performance liquid chromatographic method for the analysis of some beta-lactams employing postcolumn chemiluminescence was developed via flow injection analysis studies.

Calibration↗
PubMedDate not supplied

Some novel observations of a drug (indoprofen)--albumin interaction.

Indoprofen, a new-steroidal anti-inflammatory agent, binds to human serum albumin in an endothermic reaction at low degrees of saturation and with an exothermic reaction at higher drug to protein ratios. Although indoprofen appears to bind to the same primary site as diazepam, dialysis studies show an increased binding of both drugs in the presence of the other. This novel observation is probably due to the effect of the drugs on the N leads to B conformational change of human serum albumin.

Diazepam↗
PubMedDate not supplied

Microcalorimetric investigations of drug-albumin interactions.

Flow microcalorimetry was used to estimate primary binding constants for drug-albumin interactions. Measurements of heat of reaction at two temperatures illustrated the danger of extrapolation for pharmacokinetic purposes of measurements made at temperatures other than 37 degrees. The method could be used to predict competition between two drugs for a single binding site. Major advantages over spectroscopic techniques included direct determinations of thermodynamic parameters, and the use of physiological concentrations of albumin.

Albumins↗
PubMedDate not supplied

Albumin-binding interactions of sodium valproate.

In vitro equilibrium dialysis experiments show that salicylate and phenylbutazone displace valproate from its binding sites on human serum albumin at therapeutic concentrations, whereas warfarin and carbamazepin do not.

Binding Sites↗