Removal of bacteria and bacterial endotoxin from dialysis fluids by the media in a sorbent cartridge.
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Biomedical subjects
Publications and source records attributed to J H Marshall.
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A filter-rinse technique capable of detecting low levels of airborne hepatitis B surface antigen (HBsAg) was devised and evaluated. Laboratory tests showed the procedure to have an efficiency of 22% with a coefficient of variation of 11% and a capability of detecting as little as 5 x 10(-5) ml of aerosolized antigen positive serum in a single air sample. The technique was field-tested in a hemodialysis center serving a patient population with a high prevalence of HBsAg seropositivity. The antigen was not detected in any of 60 air samples collected under conditions favoring the occurrence of aerosols.
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A study was conducted in six pediatric wards to investigated factors that influence microbial contamination of mist therapy units. Most samples from nebulizer reservoirs were positive for gram-negative bacilli which multiplied rapidly in distilled water and reached levels as high as 3.0 X 10(8) viable microorganisms per ml. High prevalence rates of Pseudomonas aeruginosa and coliform bacteria as well as high levels of gram-negative bacilli were found to be associated with infrequent and inadequate cleaning and disinfection of the nebulizing equipment. In one of the wards exhibiting high rates, a move to new quarters permitted the implementation of improved cleaning and maintenance procedures for mist therapy units. The result was a significant reduction of microbial contamination. This study reemphasizes the role of microbiological surveillance in assuring proper care of nebulizing equipment in the hospital.